LOCUS Exported 9172 bp ds-DNA circular SYN 23-JAN-2018 DEFINITION Cut with BsmBI to insert clone of interest. ACCESSION . VERSION . KEYWORDS RNA_motif_plasmi SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9172) AUTHORS ZS TITLE Direct Submission JOURNAL Exported Jan 23, 2018 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9172 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 38..672 /label=5' LTR /note="5' LTR" misc_feature 722..858 /label=Psi /note="Psi" misc_feature 1218..2105 /label=ORF 1 (frame 3) /note="ORF 1 (frame 3)" misc_feature 1340..1573 /label=RRE /note="RRE" /note="RRE;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to thecytoplasm." misc_feature 1383..2105 /label=ORF 2 (frame 3) /note="ORF 2 (frame 3)" misc_feature 1398..2105 /label=ORF 3 (frame 3) /note="ORF 3 (frame 3)" misc_feature 2100..2217 /label=cPPT/CTS /note="cPPT/CTS" /note="cPPT/CTS;central polypurine tract and central termination sequence of HIV-1" enhancer 2400..2703 /label=CMV enhancer /note="CMV enhancer" /note="CMV enhancer;human cytomegalovirus immediate early enhancer" promoter 2704..2907 /label=CMV promoter /note="CMV promoter" /note="CMV promoter;human cytomegalovirus (CMV) immediate early promoter" misc_feature 2936..5606 /label=CCACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGAC GTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCC ACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCT CACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTG GCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCC CGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCT CCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTATCG CCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGG CCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTT CTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGC ACCTGGTGCATGACCCGCAAGCCCGGTGCCGGATCGGGAGAGGGCAGAGGAAGTCTGC TAACATGCGGTGACGTCGAGGAGAATCCTGGCCCACCGGTCGCCACCATGGTGAGCAA GGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATG GAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCT ACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGC CTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCC GCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCG TGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGA CGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCC GTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGG ACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTA CGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCC TACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAA GTAA /note="CCACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGAC" /note="Geneious type: Editing History Replacement" misc_feature 2939..3658 /label=ORF 4 (frame 2) /note="ORF 4 (frame 2)" misc_feature 3674..3688 /label=bb /note="bb" misc_feature 3696..3710 /label=bb /note="bb" misc_feature 3718..3732 /label=bb /note="bb" misc_feature 5525..5539 /label=bb /note="bb" misc_feature 5547..5561 /label=bb /note="bb" misc_feature 5569..5583 /label=bb /note="bb" misc_feature 5619..6207 /label=WPRE /note="WPRE" CDS complement(6090..6101) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa recognition and cleavage site CDS /note="Factor Xa recognition and cleavage site CDS" /note="Factor Xa site" /translation="IEGR" misc_feature complement(6208..6649) /label=3' SIN-LTR /note="3' SIN-LTR" promoter 6738..6921 /label=SV40 promoter /note="SV40 promoter" /note="SV40 promoter;SV40 enhancer and early promoter" rep_origin 6772..6907 /label=SV40 ori rep origin /note="SV40 ori rep origin" /note="SV40 ori;SV40 origin of replication" rep_origin complement(7136..7724) /direction=LEFT /label=ori rep origin /note="ori rep origin" /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7895..8755) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=bla CDS /note="bla" /note="AmpR;confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature complement(7895..8755) /label=ORF 3 (frame 1) /note="ORF 3 (frame 1)" promoter complement(8756..8860) /gene="bla" /label=bla promoter /note="bla AmpR promoter" /note="AmpR promoter" polyA_signal 8908..9042 /label=SV40 poly(A) signal polyA signal /note="SV40 poly(A) signal polyA signal" /note="SV40 poly(A) signal;SV40 polyadenylation signal" ORIGIN 1 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa 61 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag 121 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag 181 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg 241 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg 301 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag 361 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt 421 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt 481 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 541 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 601 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 661 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc 721 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg 781 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc 841 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg 901 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc 961 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta 1021 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc 1081 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata 1141 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca 1201 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag 1261 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag 1321 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca 1381 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag 1441 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca 1501 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg 1561 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc 1621 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga 1681 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat 1741 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt 1801 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag 1861 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta 1921 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca 1981 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag 2041 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat 2101 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 2161 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg 2221 gtttattaca gggacagcag agatccagtt tggttagtac cgggcccgct ctagagatcc 2281 gactcgagta ctaggatcca ttaggcggcc gcgtggataa ccgtattacc gccatgcatt 2341 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2401 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2461 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2521 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2581 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2641 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2701 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2761 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2821 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2881 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcaccat 2941 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 3001 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 3061 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 3121 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 3181 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 3241 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 3301 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 3361 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 3421 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 3481 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 3541 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 3601 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact 3661 tccctaagtc caagccctga aaaagggcaa gcttggccct gaaaaagggc aagcttggcc 3721 ctgaaaaagg gcaagcttgg agacggtaaa tgagcacaca aaatacacat gctaaaatat 3781 tatattctat gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa 3841 taattagaat ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa 3901 acgctagttt tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact 3961 tgttcctaag cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac 4021 tccaccagct gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc 4081 ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc 4141 ttcaacagct atttcacaag ctttcatgat ttcttctttt gttaataaac aattttccat 4201 aatacattta acaacatgtg atccagctgc tttttttaca gctttcatgt cttctaaaac 4261 taattcataa tttttgtctt ttaatgcacc aatatttaat accatatcaa tttctgttgc 4321 accatcttta attgcttcag aaacttcgaa tgcttttgta gctgttgtgc atgcacctag 4381 aggaaaacct acaacatttg ttattcctac atttgtgcct tttaataatt ctttacaata 4441 gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca aattcaattg cttcatcaca 4501 taattgttta atttcagctt tcgtagcatc ttgttttaat aatgtgtgat ctatatattt 4561 gtttagtttc attttttctc ctatatattc atttttaatt ttaattcttt aataatttcg 4621 tctactttaa ctttagcgtt ttgaacagat tcaccaacac ctataaaata aatttttagt 4681 ttaggttcag ttccacttgg gcgaacagca aatcatgact tatcttctaa ataaaatttt 4741 agtaagtctt gtcctggcat attatacatt ccatcgatgt agtcttcaac attaacaact 4801 ttaagtccag caatttgagt taagggtgtt gctctcaatg atttcattaa tggttcaatt 4861 tttaatttct tttcttctgg tttaaaattc aagtttaaag tgaaagtgta atatgcaccc 4921 atttctttaa ataaatcttc taaatagtct actaatgttt tattttgttt tttataaaat 4981 caagcagcct ctgctattaa tatagaagct tgtattccat ctttatctct agctgagtca 5041 tcaattacat atccataact ttcttcataa gcaaaaacaa aatttaatcc gttatcttct 5101 tctttagcaa tttctctacc cattcattta aatccagtta aagtttttac aatattaact 5161 ccatattttt catgagcgat tctatcaccc aaatcacttg ttacaaaact tgaatataga 5221 gccggatttt ttggaatgct atttaagcgt tttagatttg ataattttca atcaattaaa 5281 attggtcctg tttgatttcc atctaatctt acaaaatgac catcatgttt tattgccatt 5341 ccaaatctgt cagcatctgg gtcattcata ataataatat ctgcatcatg tttaatacca 5401 tattcaagcg gtatttttca tgcaggatca aattctggat ttggatttac aacattttta 5461 aatgtttcat cttcaaatgc atgctcttca acctcaataa cgttatatcc cgtctctagc 5521 tcttgccctg aaaaagggca gctcttgccc tgaaaaaggg cagctcttgc cctgaaaaag 5581 ggctatcgag gatattatct cgaggtgaat tcgttaacaa tcaacctctg gattacaaaa 5641 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 5701 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 5761 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 5821 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 5881 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 5941 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 6001 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 6061 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 6121 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 6181 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa 6241 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga 6301 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat 6361 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg 6421 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt 6481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6661 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc 6721 ttgacattgt ttaaaccgtc agcaaccata gtcccgcccc taactccgcc catcccgccc 6781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 6841 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 6901 ggaggcctag gcttttgcaa aaagctcgga gaggcggttt gcgtattggg cgctcttccg 6961 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7021 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7321 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7561 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8761 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8821 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8881 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 8941 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 9001 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 9061 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 9121 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aa //