LOCUS       Exported                9310 bp ds-DNA     circular SYN 18-MAY-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CAG-Avr-HPRT-R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 18, 2016 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     8..24
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    480..496
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        523..902
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        905..1180
                     /note="chicken beta-actin promoter"
     intron          1181..2189
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        2287..2305
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2313..2355
                     /note="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2369..5380
                     /codon_start=1
                     /note="TALEN cds"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAAVDLRT
                     LGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVALSQHPAALGTVAVKYQDMIAA
                     LPEATHEAIVGVGKQWSGARALEALLTVAGELRGPPLQLDTGQLLKIAKRGGVTAVEAV
                     HAWRNALTGAPLNLTPDQVVAIASNIGGKQALETVQRLLPVLCQDHGLTPEQVVAIASN
                     GGGKQALETVQRLLPVLCQAHGLTPDQVVAIASNIGGKQALETVQRLLPVLCQAHGLTP
                     AQVVAIASHDGGKQALETVQRLLPVLCQDHGLTPDQVVAIASNGGGKQALETVQRLLPV
                     LCQDHGLTPEQVVAIASHDGGKQALETVQRLLPVLCQAHGLTPDQVVAIASNIGGKQAL
                     ETVQRLLPVLCQAHGLTPAQVVAIASHDGGKQALETVQRLLPVLCQDHGLTPDQVVAIA
                     SNIGGKQALETVQRLLPVLCQDHGLTPEQVVAIASHDGGKQALETVQRLLPVLCQAHGL
                     TPDQVVAIASNIGGKQALETVQRLLPVLCQAHGLTPAQVVAIASNIGGKQALETVQRLL
                     PVLCQDHGLTPDQVVAIASNGGGKQALETVQRLLPVLCQDHGLTPEQVVAIASNIGGKQ
                     ALETVQRLLPVLCQAHGLTPDQVVAIASNNGGKQALETVQRLLPVLCQAHGLTPAQVVA
                     IASHDGGKQALETVQRLLPVLCQDHGLTPEQVVAIASNGGGRPALESIVAQLSRPDPAL
                     AALTNDHLVALACLGGRPALDAVKKGLPHAPALIKRTNRRIPERTSHRVAGSQLVKSEL
                     EEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKP
                     DGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVEENQTRNKHINPNEWWKVY
                     PSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLSVEELLIGGEMIKAGTLTLEE
                     VRRKFNNGEINFRS"
     CDS             2372..2437
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2444..2464
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             4784..5371
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /note="FokI cleavage domain"
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     polyA_signal    5395..5506
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5629..5958
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      5809..5944
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             6007..7032
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7162..7283
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(7336..8196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8197..8288)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      8460..9048
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgtgttgta aaacgacggc cagtcttaag ctcgggcccc ctgggcggtt ctgataacga
       61 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga
      121 gtttagggaa agagcatttg tcagaatatt taagggcgcc tgtcactttg cttgatatat
      181 gagaattatt taaccttata aatgagaaaa aagcaacgca ctttaaataa gatacgttgc
      241 tttttcgatt gatgaacacc tataattaaa ctattcatct attatttatg attttttgta
      301 tatacaatat ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa aataataaag
      361 ggaaaatcag tttttgatat caaaattata catgtcaacg ataatacaaa atataataca
      421 aactataaga tgttatcagt atttattatc atttagaata aattttgtgt cgcccttaat
      481 tgtgagcgga taacaattac gagcttcatg cacagtggcg ttgacattga ttattgacta
      541 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      601 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      661 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      721 gggtggacta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      781 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      841 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      901 tgggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      961 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
     1021 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     1081 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     1141 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc
     1201 gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     1261 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     1321 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg
     1381 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     1441 ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1501 tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1561 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1621 tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac
     1681 ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc
     1741 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1801 tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1861 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1921 ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg
     1981 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     2041 ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt cgggggggac
     2101 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     2161 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt
     2221 ctcatcattt tggcaaagaa ttctctggct aactagagaa cccactgctt actggcttat
     2281 cgaaattaat acgactcact atagggaagc ttcttgttct ttttgcagaa gctcagaata
     2341 aacgctcaac tttggcctcg aggccaccat ggactataag gaccacgacg gagactacaa
     2401 ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa
     2461 ggtcggtatc cacggagtcc cagcagccgt agatttgaga actttgggat attcacagca
     2521 gcagcaggaa aagatcaagc ccaaagtgag gtcgacagtc gcgcagcatc acgaagcgct
     2581 ggtgggtcat gggtttacac atgcccacat cgtagccttg tcgcagcacc ctgcagccct
     2641 tggcacggtc gccgtcaagt accaggacat gattgcggcg ttgccggaag ccacacatga
     2701 ggcgatcgtc ggtgtgggga aacagtggag cggagcccga gcgcttgagg ccctgttgac
     2761 ggtcgcggga gagctgagag ggcctcccct tcagctggac acgggccagt tgctgaagat
     2821 cgcgaagcgg ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca atgcgctcac
     2881 gggagcaccc ctcaacctga ccccagacca ggttgtggcc atcgccagca acataggtgg
     2941 caagcaggcc ctcgaaaccg tccagagact gttaccggtt ctctgccagg accacggcct
     3001 gacccccgaa caggttgtcg ctattgctag taacggcgga ggcaaacagg cgctggaaac
     3061 agttcagcgc ctcttgccgg tcttgtgtca ggcccacggc ctgaccccag accaggttgt
     3121 ggccatcgcc agcaacatag gtggcaagca ggccctcgaa accgtccaga gactgttacc
     3181 ggttctctgc caggcccacg gcctgacccc ggcccaggtg gttgcaatcg cgtcacacga
     3241 tgggggaaag caggccctag aaaccgttca gcgactcctg cccgtcctgt gccaggacca
     3301 cggcctgacc cccgaccagg ttgtcgctat tgctagtaac ggcggaggca aacaggcgct
     3361 ggaaacagtt cagcgcctct tgccggtctt gtgtcaggac cacggcctga ccccggaaca
     3421 ggtggttgca atcgcgtcac acgatggggg aaagcaggcc ctagaaaccg ttcagcgact
     3481 cctgcccgtc ctgtgccagg cccacggcct gaccccagac caggttgtgg ccatcgccag
     3541 caacataggt ggcaagcagg ccctcgaaac cgtccagaga ctgttaccgg ttctctgcca
     3601 ggcccacggc ctgaccccgg cccaggtggt tgcaatcgcg tcacacgatg ggggaaagca
     3661 ggccctagaa accgttcagc gactcctgcc cgtcctgtgc caggaccacg gcctgacccc
     3721 agaccaggtt gtggccatcg ccagcaacat aggtggcaag caggccctcg aaaccgtcca
     3781 gagactgtta ccggttctct gccaggacca cggcctgacc ccggaacagg tggttgcaat
     3841 cgcgtcacac gatgggggaa agcaggccct agaaaccgtt cagcgactcc tgcccgtcct
     3901 gtgccaggcc cacggcctga ccccagacca ggttgtggcc atcgccagca acataggtgg
     3961 caagcaggcc ctcgaaaccg tccagagact gttaccggtt ctctgccagg cccacggcct
     4021 gaccccagcc caggttgtgg ccatcgccag caacataggt ggcaagcagg ccctcgaaac
     4081 cgtccagaga ctgttaccgg ttctctgcca ggaccacggc ctgacccccg accaggttgt
     4141 cgctattgct agtaacggcg gaggcaaaca ggcgctggaa acagttcagc gcctcttgcc
     4201 ggtcttgtgt caggaccacg gcctgacccc agaacaggtt gtggccatcg ccagcaacat
     4261 aggtggcaag caggccctcg aaaccgtcca gagactgtta ccggttctct gccaggccca
     4321 cggcctgacc ccagaccaag ttgtcgcgat tgcaagcaac aacggaggca aacaagcctt
     4381 agaaacagtc cagagattgt tgccggtgct gtgccaagcc cacggcctga ccccggccca
     4441 ggtggttgca atcgcgtcac acgatggggg aaagcaggcc ctagaaaccg ttcagcgact
     4501 cctgcccgtc ctgtgccagg accacggcct gacgcctgag caggtagtgg ctattgcatc
     4561 caacggaggg ggcagacccg cactggagtc aatcgtggcc cagctttcga ggccggaccc
     4621 cgcgctggcc gcactcacta atgatcatct tgtagcgctg gcctgcctcg gcggacgacc
     4681 cgccttggat gcggtgaaga aggggctccc gcacgcgcct gcattgatta agcggaccaa
     4741 cagaaggatc cccgagagga catcacatcg agtggcaggt tcccaactcg tgaagagtga
     4801 acttgaggag aaaaagtcgg agctgcggca caaattgaaa tacgtaccgc atgaatacat
     4861 cgaacttatc gaaattgcta ggaactcgac tcaagacaga atccttgaga tgaaggtaat
     4921 ggagttcttt atgaaggttt atggataccg agggaagcat ctcggtggat cacgaaaacc
     4981 cgacggagca atctatacgg tggggagccc gattgattac ggagtgatcg tcgacacgaa
     5041 agcctacagc ggtgggtaca atcttcccat cgggcaggca gatgagatgc aacgttatgt
     5101 cgaagaaaat cagaccagga acaaacacat caatccaaat gagtggtgga aagtgtatcc
     5161 ttcatcagtg accgagttta agtttttgtt tgtctctggg catttcaaag gcaactataa
     5221 ggcccagctc acacggttga atcacattac gaactgcaat ggtgcggttt tgtccgtaga
     5281 ggaactgctc attggtggag aaatgatcaa agcgggaact ctgacactgg aagaagtcag
     5341 acgcaagttt aacaatggcg agatcaattt ccgctcataa aaaatcagcc tcgactgtgc
     5401 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5461 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa cactcaaccc
     5521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5581 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5641 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5761 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5881 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5941 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     6001 cacgtgatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     6061 ttcgacagcg tttccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     6121 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     6181 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     6241 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     6301 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     6361 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     6421 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     6481 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     6541 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     6601 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     6661 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     6721 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     6781 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     6841 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     6901 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     6961 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     7021 gcaaaggaat agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7081 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7141 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7201 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7261 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7321 gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7441 cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga
     7501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7621 taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt
     7681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8161 gggaataagg gcgacacgga aatgttgaat actcatattc ttcctttttc aatattattg
     8221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8281 taaacaaata ggggtcagtg ttacaaccaa ttaaccaatt ctgaacatta tcgcgagccc
     8341 atttatacct gaatatggct cataacaccc cttgctcatg accaaaatcc cttaacgtga
     8401 gttacgcgcg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8461 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8521 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8581 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagc ccaccacttc
     8641 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8701 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8761 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8821 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8881 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8941 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9001 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9061 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaagg cgagagtagg
     9241 gaactgccag gcatcaaact aagcagaagg cccctgacgg atggcctttt tgcgtttcta
     9301 caaactcttt
//
