LOCUS Exported 6251 bp ds-DNA circular SYN 18-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6251) AUTHORS GN TITLE Direct Submission JOURNAL Exported Jan 25, 2018 from SnapGene 4.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6251 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" misc_feature 169..644 /label=hSyn misc_feature 169..178 /label=Feature 16 misc_feature 634..644 /label=Feature 17 CDS 669..1376 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 669..689 /label=SH-132 misc_feature 1357..1376 /label=SH 133 CDS 1392..1448 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 1449..2495 /label=cre CDS 1449..1469 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2496..2522 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 2556..3144 /label=WPRE misc_feature 2556..3143 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(3027..3038) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" misc_feature 3185..3410 /label=BGH polyA polyA_signal 3185..3409 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" misc_feature 3418..3431 /label=Forward Primer repeat_region 3514..3654 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3514..3654 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 3729..4184 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4466..4570 /gene="bla" /label=AmpR promoter CDS 4571..5431 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5602..6190 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca atcgatttca tatgccggct gcagagggcc 181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct 241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc 301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc 361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac 421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg 481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg 541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt 601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg agaaatcacc tccgctgctc 661 gcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 721 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 781 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 841 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 901 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 961 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 1021 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 1081 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 1141 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 1201 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 1261 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 1321 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcta 1381 gcggaagcgg agccactaac ttctccctgt tgaaacaagc aggggatgtc gaagagaatc 1441 ccgggccacc caagaagaag aggaaggtgt ccaatctcct gactgttcac cagaacctcc 1501 ctgcgctgcc agtagatgcc actagcgatg aggtcaggaa aaatctcatg gatatgttta 1561 gggatagaca ggcgttttct gaacacacct ggaaaatgct gcttagcgtg tgccgatcct 1621 gggcagcctg gtgtaagctg aacaatcgca aatggttccc cgccgagccg gaggacgtgc 1681 gcgattacct gctgtatctc caggcaagag ggctggctgt caagactatc cagcagcact 1741 tgggccaact gaatatgctg catcgacgca gcgggctccc ccggcctagc gattcaaacg 1801 cagtctccct tgttatgagg agaattagaa aggaaaacgt agatgcgggt gagagggcta 1861 agcaggctct cgcttttgag cggactgatt tcgaccaggt cagatccctg atggagaaca 1921 gcgatcggtg ccaggacatc aggaacctcg catttctggg aattgcatat aacacacttc 1981 tgcgcatagc tgagatcgcc cggatcagag tgaaagacat cagtcgaacg gacggcggcc 2041 ggatgcttat tcatattgga cgcacaaaga cattggtcag caccgctggc gttgaaaagg 2101 ccttgtccct gggcgtaacg aagctggtgg aaagatggat ctcagtgtcc ggcgtggctg 2161 acgaccctaa taattacttg ttctgtcgag tgagaaaaaa cggagtcgcc gcgccctctg 2221 ccaccagcca attgagtaca cgggcccttg aagggatctt tgaggcaacc caccgactca 2281 tatacggagc caaggatgac agtggccaga ggtatctcgc ctggtcaggt cattctgcta 2341 gggtgggggc cgcacgagac atggcgcggg caggagtctc cataccagag attatgcaag 2401 ctggaggttg gacaaatgtg aacatcgtta tgaactatat ccgcaatctt gactctgaaa 2461 ccggggccat ggtgagactg ctcgaagatg gtgactaccc atacgatgtt ccagattacg 2521 cttaagaatt cgatatcaag cttatcgata agtaaaatca acctctggat tacaaaattt 2581 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 2641 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 2701 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 2761 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 2821 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 2881 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 2941 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 3001 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 3061 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga 3121 tctccctttg gccgcctccc cgccaccggt taggggcccg tttaaacccg ctgatcagcc 3181 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 3241 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 3301 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 3361 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ggcgcgtcca 3421 gtgtgctgga aataccgagc gctaataaaa gatctttatt ttcattagat ctgtgtgttg 3481 gttttttgtg tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 3541 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 3601 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 3661 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 3721 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 3781 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 3841 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 3901 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 3961 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4021 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt 4081 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 4141 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 4201 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 4261 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 4321 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 4381 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 4441 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 4501 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 4561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 4621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 4681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 4741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 4801 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 4861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 4921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 4981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 5041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 5101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 5161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 5221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 5281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 5341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 5401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 5461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 5521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 5581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 5641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 5701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 5761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 5821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 5881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 5941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 6001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 6061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 6121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 6181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 6241 tgctcacatg t //