LOCUS Exported 7075 bp ds-DNA circular SYN 18-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7075) AUTHORS GN TITLE Direct Submission JOURNAL Exported Jan 25, 2018 from SnapGene 4.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7075 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" misc_feature 169..1461 /label=aCamkII promoter CDS 1493..2200 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 1493..1513 /label=SH-132 misc_feature 2181..2200 /label=SH 133 CDS 2216..2272 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 2273..3319 /label=cre CDS 2273..2293 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3320..3346 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 3380..3968 /label=WPRE misc_feature 3380..3967 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(3851..3862) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" misc_feature 4009..4234 /label=BGH polyA polyA_signal 4009..4233 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" misc_feature 4242..4255 /label=Forward Primer repeat_region 4338..4478 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4338..4478 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 4553..5008 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5290..5394 /gene="bla" /label=AmpR promoter CDS 5395..6255 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6426..7014 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca atcgatttca tatgccggtt aacattatgg 181 ccttaggtca cttcatctcc atggggttct tcttctgatt ttctagaaaa tgagatgggg 241 gtgcagagag cttcctcagt gacctgccca gggtcacatc agaaatgtca gagctagaac 301 ttgaactcag attactaatc ttaaattcca tgccttgggg gcatgcaagt acgatataca 361 gaaggagtga actcattagg gcagatgacc aatgagttta ggaaagaaga gtccagggca 421 gggtacatct acaccacccg cccagccctg ggtgagtcca gccacgttca cctcattata 481 gttgcctctc tccagtccta ccttgacggg aagcacaagc agaaactggg acaggagccc 541 caggagacca aatcttcatg gtccctctgg gaggatgggt ggggagagct gtggcagagg 601 cctcaggagg ggccctgctg ctcagtggtg acagataggg gtgagaaagc agacagagtc 661 attccgtcag cattctgggt ctgtttggta cttcttctca cgctaaggtg gcggtgtgat 721 atgcacaatg gctaaaaagc agggagagct ggaaagaaac aaggacagag acagaggcca 781 agtcaaccag accaattccc agaggaagca aagaaaccat tacagagact acaaggggga 841 agggaaggag agatgaatta gcttcccctg taaaccttag aacccagctg ttgccagggc 901 aacggggcaa tacctgtctc ttcagaggag atgaagttgc cagggtaact acatcctgtc 961 tttctcaagg accatcccag aatgtggcac ccactagccg ttaccatagc aactgcctct 1021 ttgccccact taatcccatc ccgtctgtta aaagggccct atagttggag gtgggggagg 1081 taggaagagc gatgatcact tgtggactaa gtttgttcgc atccccttct ccaaccccct 1141 cagtacatca ccctggggga acagggtcca cttgctcctg ggcccacaca gtcctgcagt 1201 attgtgtata taaggccagg gcaaagagga gcaggtttta aagtgaaagg caggcaggtg 1261 ttggggaggc agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg 1321 acctggatgc tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc 1381 cccaagctcg tcagtcaagc cggttctccg tttgcactca ggagcacggg caggcgagtg 1441 gcccctagtt ctgggggcag cgctcgtaat cacctccgct gctcgcgcca ccatggtgag 1501 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat 1561 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 1621 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg 1681 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga 1741 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 1801 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt 1861 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa 1921 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa 1981 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa 2041 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa 2101 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga 2161 gggccgccac tccaccggcg gcatggacga gctgtacaag gctagcggaa gcggagccac 2221 taacttctcc ctgttgaaac aagcagggga tgtcgaagag aatcccgggc cacccaagaa 2281 gaagaggaag gtgtccaatc tcctgactgt tcaccagaac ctccctgcgc tgccagtaga 2341 tgccactagc gatgaggtca ggaaaaatct catggatatg tttagggata gacaggcgtt 2401 ttctgaacac acctggaaaa tgctgcttag cgtgtgccga tcctgggcag cctggtgtaa 2461 gctgaacaat cgcaaatggt tccccgccga gccggaggac gtgcgcgatt acctgctgta 2521 tctccaggca agagggctgg ctgtcaagac tatccagcag cacttgggcc aactgaatat 2581 gctgcatcga cgcagcgggc tcccccggcc tagcgattca aacgcagtct cccttgttat 2641 gaggagaatt agaaaggaaa acgtagatgc gggtgagagg gctaagcagg ctctcgcttt 2701 tgagcggact gatttcgacc aggtcagatc cctgatggag aacagcgatc ggtgccagga 2761 catcaggaac ctcgcatttc tgggaattgc atataacaca cttctgcgca tagctgagat 2821 cgcccggatc agagtgaaag acatcagtcg aacggacggc ggccggatgc ttattcatat 2881 tggacgcaca aagacattgg tcagcaccgc tggcgttgaa aaggccttgt ccctgggcgt 2941 aacgaagctg gtggaaagat ggatctcagt gtccggcgtg gctgacgacc ctaataatta 3001 cttgttctgt cgagtgagaa aaaacggagt cgccgcgccc tctgccacca gccaattgag 3061 tacacgggcc cttgaaggga tctttgaggc aacccaccga ctcatatacg gagccaagga 3121 tgacagtggc cagaggtatc tcgcctggtc aggtcattct gctagggtgg gggccgcacg 3181 agacatggcg cgggcaggag tctccatacc agagattatg caagctggag gttggacaaa 3241 tgtgaacatc gttatgaact atatccgcaa tcttgactct gaaaccgggg ccatggtgag 3301 actgctcgaa gatggtgact acccatacga tgttccagat tacgcttaag aattcgatat 3361 caagcttatc gataagtaaa atcaacctct ggattacaaa atttgtgaaa gattgactgg 3421 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 3481 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 3541 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 3601 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 3661 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 3721 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 3781 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 3841 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 3901 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc 3961 tccccgccac cggttagggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta 4021 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 4081 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 4141 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 4201 gcaggcatgc tggggatgcg gtgggctcta tggcggcgcg tccagtgtgc tggaaatacc 4261 gagcgctaat aaaagatctt tattttcatt agatctgtgt gttggttttt tgtgtcacgt 4321 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc 4381 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 4441 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct 4501 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc 4561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 4621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 4681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 4741 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc 4801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 4861 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt 4921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 4981 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat 5041 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 5101 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 5161 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 5221 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 5281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 5341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 5401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 5461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 5641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 5941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 6001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 6121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6541 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 6601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 7021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt //