LOCUS       pEXP5-CT-aHL        3567 bp    DNA     circular     24-SEP-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|635790404|
COMMENT     VNTDBDATE|638021534|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXP5-CT-aHL|
COMMENT     VNTAUTHORNAME|Sergey Kalachikov|
COMMENT     VNTAUTHORTEL|(212)851-9193|
COMMENT     VNTAUTHOREML|sk363@columbia.edu|
FEATURES             Location/Qualifiers
     promoter        1..20
                     /dnas_title="T7 Promoter"
                     /vntifkey="30"
                     /label=T7 Promoter
     RBS             69..74
                     /dnas_title="RBS"
                     /vntifkey="32"
                     /label=RBS
     CDS             1033..1056
                     /dnas_title="6xHis region"
                     /vntifkey="4"
                     /label=6xHis region
     misc_marker     1057..1059
                     /dnas_title="Stop Codon"
                     /vntifkey="22"
                     /label=Stop Codon
     misc_feature    1094..1178
                     /dnas_title="T7 transcription terminator"
                     /vntifkey="21"
                     /label=T7 transcription terminator
     promoter        1290..1388
                     /dnas_title="bla Promoter"
                     /vntifkey="30"
                     /label=bla Promoter
     CDS             1389..2249
                     /dnas_title="AmpR"
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    2394..3067
                     /dnas_title="pUC Origin"
                     /vntifkey="21"
                     /label=pUC Origin
     CDS             85..963
                     /dnas_title="Mature aHL"
                     /vntifkey="4"
                     /label=Mature aHL
     variation       907..909
                     /dnas_title="T275I"
                     /vntifkey="96"
                     /label=T275I
     variation       706..708
                     /dnas_title="E208D"
                     /vntifkey="96"
                     /label=E208D
     primer          complement(940..963)
                     /dnas_title="aHLR"
                     /vntifkey="27"
                     /label=aHLR
     primer          85..112
                     /dnas_title="aHLF"
                     /vntifkey="27"
                     /label=aHLF
     variation       106..108
                     /dnas_title="Protease resistance site (K8A)"
                     /vntifkey="96"
                     /label=Protease resistance site (K8A)
     variation       133..135
                     /dnas_title="Attachment site N17C"
                     /vntifkey="96"
                     /label=Attachment site N17C
     variation       220..222
                     /dnas_title="Attachment site K46C"
                     /vntifkey="96"
                     /label=Attachment site K46C
     primer          1..20
                     /dnas_title="T7 Forward (LTech's)"
                     /vntifkey="27"
                     /label=T7 Forward (LTech's)
     primer          complement(1156..1178)
                     /dnas_title="T7 Term Reverse (LTech's)"
                     /vntifkey="27"
                     /label=T7 Term Reverse (LTech's)
     primer          3587..3604
                     /dnas_title="pExpF (72.3)"
                     /vntifkey="27"
                     /label=pExpF (72.3)
     primer          complement(1198..1225)
                     /dnas_title="pExpR (71)"
                     /vntifkey="27"
                     /label=pExpR (71)
     primer          complement(133..163)
                     /dnas_title="wtN17C"
                     /vntifkey="27"
                     /label=wtN17C
     primer          complement(220..249)
                     /dnas_title="wtK46C"
                     /vntifkey="27"
                     /label=wtK46C
     PCR_primer      complement(940..963)
                     /dnas_title="ATTTGTCATTTCTTCTTTTTCCCA"
                     /pair=""
                     /primer="ATTTGTCATTTCTTCTTTTTCCCA"
                     /current=0
     source          1..3636
                     /dnas_title="pEXP5-CT-aHL"
     PCR_primer      67..111
                     /dnas_title="GAAGGAGATACCCTTATGGCAGATTCTGATATTAATATTAAAACC
                     "
                     /pair="aHLNterm_to_pEXP5_F"
                     /primer="GAAGGAGATACCCTTATGGCAGATTCTGATATTAATATTAAAACC"
                     /current=0
     misc_feature    994..1032
                     /note="SpyTag"
     misc_feature    964..993
                     /note="Linker"
     PCR_primer      complement(941..999)
                     /dnas_title="GTGTGCACCACCACTACTGCCACCACTGCTGCCGCCATTTGTCAT
                     TTCTTCTTTTTCCC"
                     /pair="aHL_SpyTag_His R"
                     /primer="GTGTGCACCACCACTACTGCCACCACTGCTGCCGCCATTTGTCATTTCT
                     TCTTTTTCCC"
                     /current=0
     PCR_primer      1000..1058
                     /dnas_title="ATTGTGATGGTTGATGCGTACAAACCGACCAAGAAGGGTCATCAT
                     CACCATCACCATTG"
                     /pair="aHL_SpyTag_His F"
                     /primer="ATTGTGATGGTTGATGCGTACAAACCGACCAAGAAGGGTCATCATCACC
                     ATCACCATTG"
                     /current=1
ORIGIN      
        1 taatacgact cactataggg agacacacga cggtttccct ctagaaataa ttttgtttaa
       61 ctttaagaag gagataccct tatggcagat tctgatatta atattaaaac cggtactaca
      121 gatattggaa gcaatactac agtaaaaaca ggtgatttag tcacttatga taaagaaaat
      181 ggcatgcaca aaaaagtatt ttatagtttt atcgatgata aaaatcacaa taaaaaactg
      241 ctagttatta gaacgaaagg taccattgct ggtcaatata gagtttatag cgaagaaggt
      301 gctaacaaaa gtggtttagc ctggccttca gcctttaagg tacagttgca actacctgat
      361 aatgaagtag ctcaaatatc tgattactat ccaagaaatt cgattgatac aaaagagtat
      421 atgagtactt taacttatgg attcaacggt aatgttactg gtgatgatac aggaaaaatt
      481 ggcggcctta ttggtgcaaa tgtttcgatt ggtcatacac tgaaatatgt tcaacctgat
      541 ttcaaaacaa ttttagagag cccaactgat aaaaaagtag gctggaaagt gatatttaac
      601 aatatggtga atcaaaattg gggaccatat gatagagatt cttggaaccc ggtatatggc
      661 aatcaacttt tcatgaaaac tagaaatggt tctatgaaag cagcagagaa cttccttgat
      721 cctaacaaag caagttctct attatcttca gggttttcac cagacttcgc tacagttatt
      781 actatggata gaaaagcatc caaacaacaa acaaatatag atgtaatata cgaacgagtt
      841 cgtgatgact accaattgca ttggacttca acaaattgga aaggtaccaa tactaaagat
      901 aaatggacag atcgttcttc agaaagatat aaaatcgatt gggaaaaaga agaaatgaca
      961 aatGGCGGCA GCAGTGGTGG CAGTAGTGGT GGTGCACACA TTGTGATGGT TGATGCGTAC
     1021 AAACCGACCA AGaagggtca tcatcaccat caccattgag tttaaactat atagaataaa
     1081 agaagaaacc ttagctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     1141 gaggggtttt ttgctgaaag gaggaactat atccggataa ttcttgaaga cgaaagggcc
     1201 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1261 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1621 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     1801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2101 gtgggtcacg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     2821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3121 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3181 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3241 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     3301 ccgctatcgc tacgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     3361 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     3421 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     3481 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     3541 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     3601 gacccaacgc tgcccgagat ctcgatcccg cgaaat
//

