LOCUS       Exported                6909 bp ds-DNA     circular SYN 24-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDis Rncp-iGluSnFR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, February 1, 2018 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        466..845
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        846..1049
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1094..1112
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1193..1255
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /note="Ig-kappa leader"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    1262..2902
                     /note="Rncp-iGluSnFR1"
     CDS             1796..1813
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             1817..1849
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1853..1876
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /note="Xpress(TM) tag"
                     /translation="DLYDDDDK"
     CDS             2915..2944
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     polyA_signal    3122..3346
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3478..4066)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4395..4442
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4674..5468)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5916..6776)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6777..6881)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      6908..454
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
       61 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      361 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      421 gcgaatttta acaaaatatt aacgcttaca atttacgcgc gcgttgacat tgattattga
      481 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      541 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      601 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      661 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      721 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      781 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      841 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      901 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      961 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     1021 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     1081 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga
     1141 tccactagta acggccgcca gtgtgctgga attcggcttg gggatatcca ccatggagac
     1201 agacacactc ctgctatggg tactgctgct ctgggttcca ggttccactg gtgacagatc
     1261 tgtgagcaag ggcgaggggg ataacaaggc catcatcaag gagttcatgc gcttcaaggt
     1321 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1381 cccctacgag gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt
     1441 cgcctgggac atcctgtctc ctcagttcat gtacggctcc aaggcctaca ttaagcaccc
     1501 agccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt
     1561 gatgatcttc gaggacggcg gcattattca cgttagccag gactcctccc tgcaggacgg
     1621 cgtattcatc tacaaggtga agctgcgcgg caccaacttc ccccccgacg gccccgtaat
     1681 gcagaagaag accatgggct gggagcctac gcgtctgaac atgaatttcg aactgtcaga
     1741 cgaaatgaaa gcacggttca aagaaccgaa tgacaaggca ctgaaaggcg gctctcatca
     1801 tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta
     1861 cgacgatgac gataaggatc ctggctcgag cggatccgcc gcaggcagca cgctggacaa
     1921 aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtgc ctttctcttt
     1981 ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg ccattgttga
     2041 agcagtgaag aagaaactca acaaaccgga cttgcaggta aaactgattc cgattacctc
     2101 acaaaaccgt attccactgc tgcaaaacgg cactatcgat tttgaatgtg gttctaccac
     2161 caacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac
     2221 gcgcctgttg accaaaaagg gtggcgatat caaagatttt gccaacctga aagacaaagc
     2281 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
     2341 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
     2401 aagcggtcgt gccgttgcct ttatgatgga tgacgtgctg ctggccggtg aacgtgcgaa
     2461 agcgaagaaa ccagacaact gggaaatcgt cggcaagccg cagtctctgg aggcctacgg
     2521 ttgtatgttg cgtaaagatg atccgcagtt caaaaagctg gtggatgaca ccatcgctca
     2581 ggtgcagacc tccggtgaag cggaaaaatg gtttgataag tggttcaaaa atccaattga
     2641 ggtggtttcc gagcggatgt accccgagga cggcgccctg aagagcgtga tcaggaaggg
     2701 gctgaggctg aaggacggcg gccactacgc cgccgaggcc aagaccacct acaaggccaa
     2761 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
     2821 caacgaggac tacaccatcg tggaacagtg cgaacgcgcc gagggccgcc actccaccgg
     2881 cggcatggac gagctgtaca agctgcaggt cgacgaacaa aaactcatct cagaagagga
     2941 tctgaatgct gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt
     3001 taaggtggtg gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat
     3061 catcctcatc atgctttggc agaagaagcc acgttaggcg gccgctcgag atcagcctcg
     3121 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3181 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3241 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3301 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3361 agaaccagtg gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4201 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     4261 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     4321 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     4381 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     4441 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     4501 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     4561 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     4621 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     4681 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     4741 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     4801 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     4861 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4921 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4981 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     5041 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     5101 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     5161 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     5221 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     5281 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     5341 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     5401 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     5461 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     5521 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgggcctc ggcctctgca
     5581 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     5641 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     5701 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     5761 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     5821 tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt caataaatca
     5881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6181 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6241 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6301 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6361 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6421 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6481 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6541 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6601 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6661 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6721 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6781 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6841 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6901 ccacctgac
//
