LOCUS       1.(N21)PthrC(RBS1)pureG 3124 bp ds-DNA     circular     09-JAN-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2126..2144
                     /label="N23"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    98..116
                     /label="N22"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1238..1948
                     /label="pure\eGFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1005..1023
                     /label="N21"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1024..1194
                     /label="PthrC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1024..1043
                     /label="PthrC-f"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1195..1237
                     /label="RBS1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             169..757
                     /label="pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1970..2125
                     /label="pheA terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1949..1969
                     /label="N31"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1171..1180
                     /label="thrLp trans start"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1173..1194
                     /label="PthrC-r"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2310..3102
                     /label="aadA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ctgagctcat gaagttccta ttccgaagtt ccgcgaacgc gtaaaggatc taggtgaaga
       61 tcctttttga taatctcatg accaaaatcc cttaacgtca cacaacatag ccacggagtt
      121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      301 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      421 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      541 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      601 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      661 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      721 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      781 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      841 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      901 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
      961 ccttacgcat ctgtgcggta tttcacaccg catatgctgg atccTccctc gactcacact
     1021 tgGagctttt cattctgact gcaacgggca atatgtctct gtgtggatta aaaaaagagt
     1081 gtctgatagc agcttctgaa ctggttacct gccgtgagta aattaaaatt ttattgactt
     1141 aggtcactaa atactttaac caatataggc atagcgcaca gacagataaa aatttagaaa
     1201 taattttgtt taactttaag aaggagatat acatatgagc aagggcgagg agctgttcac
     1261 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1321 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1381 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1441 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1501 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1561 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1621 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1681 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1741 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1801 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1861 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1921 cactctcggc atggacgagc tgtacaagTg atgggctgaa gggtttaaGC GAAGACGAAC
     1981 AATAAGGCCT CCCAAATCGG GGGGCCTTTT TTATTGATAA CAAAAAGGCA ACACTATGAC
     2041 ATCGGAAAAC CCGTTACTGG CGCTGCGAGA GAAAATCAGC GCGCTGGATG AAAAATTATT
     2101 AGCATTACTG GCAGAGCGGC GCGAAtcaaa cagaaaggcc atggtcgacc tgcagaagct
     2161 tagatctatt accctgttat ccctactcga gttcatgtgc agctccatca gcaaaagggg
     2221 atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac
     2281 tgatgtcatc agcggtggag tgcaatgtca tgagggaagc ggtgatcgcc gaagtatcga
     2341 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2401 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2461 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2521 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2581 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2641 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2701 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2761 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2821 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2881 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2941 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     3001 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     3061 actacgtgaa aggcgagatc accaaggtag tcggcaaata agatgccgct cgccagtcga
     3121 ttgg
//