LOCUS       Exported                3759 bp ds-DNA     circular SYN 31-JAN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    FoxFTVC+bpFOG_MRAS-G22V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, February 1, 2018 from SnapGene Viewer 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..303
                     /label=FoxF TVC
                     /note="FoxF TVC"
                     /note="color: #804040"
     misc_feature    326..536
                     /label=bpFOG -1
                     /note="bpFOG -1"
                     /note="color: #408080"
     misc_feature    549..1148
                     /label=caMRAS_ORF
                     /note="caMRAS_ORF"
                     /note="color: #00ffff"
     misc_feature    612..614
                     /label=ca G>V
                     /note="GGT>GTT_G>V Mutation"
                     /note="color: #999999"
     polyA_signal    1485..1619
                     /note="pA-SV40"
                     /note="color: #a6acb3"
     misc_feature    complement(1656..1674)
                     /note="T7 promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(1932..2520)
                     /note="ori"
                     /note="color: #ffff00; direction: LEFT"
     CDS             complement(2694..3551)
                     /codon_start=1
                     /note="AmpR"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(3552..3656)
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggcgcgccgc gttgtatctt tcgttaagtg ctggcgtcga gcagggctaa cctcatttcg
       61 atttgacgat tcgggtaaaa ccgagcgatg gacaggacct ggaaatggga aaggcttact
      121 agaatcaggg tcgaacgaga taagaagatc aggattcgca aattagcact tggctttttt
      181 acgtagaaac ataggatgtc cgctgagccc cttatatata cgtagtacga atcaccggcc
      241 tggcgttgta taagcgagac cagcgtggac gaaaaggtaa ttgtttccga atggatcgcg
      301 ttctctagag gatccggcaa agcttcgtgt attgtaccgg cccattgtca atcatgcaaa
      361 cttgatatta tattgacaag agaagaaggc agtttaaatt aaaactctaa agtagagaga
      421 cattaatctc agctgacaag gcaggtggtc acagtaagtt catttaaata gttggccaac
      481 aatagccttt ccaagaaagt atttttgttc caggtctata caaaaataac acacaagcgg
      541 ccgcaaccat ggcgaccgtg ccgaatcaga atgagaactt aacgacatat aagttggtcg
      601 tggttggcga tgttggtgtc gggaagagcg cgctcaccat ccaattcttc caaaagatgt
      661 tcgttgaaga atatgatcca actatagagg attcatatat ccaacacaca gatatcgatg
      721 gacagtggtg tattttagat gtgctcgaca cagccggcca ggaggaattt agtgcaatgc
      781 gtgaacaata catgagaaca ggtgatggtt tccttatcgt gtacagcgta acggaccgcg
      841 caagttacga tcacgtacca gagttccacg atcttattct aagggtaaaa gatgaaacac
      901 aattccccgc cctcctagtg gccaacaaag ttgatcttgt gcaccagcga gtggtggaag
      961 aagacgaggg gaaggaaatg gcgcgacaat tccagattcc ttacattgaa acgagcgcca
     1021 aagaccctcc gcaaaacgtt gataaagctt tccaagatat tgttcgttta ataaggcaac
     1081 aaggtcttca acaaaacggg acgaccaccg gaggtcggga gagcaaagga tgttgtcagg
     1141 ttatgtagct ccacctagag gccagaattc cagctgagcg ccggtcgcta ccattaccag
     1201 ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     1261 aggaaatcca ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt
     1321 ttcttttact tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac
     1381 atcaaccata tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg
     1441 ctattattcc aaccgctgtt tggtctgctt tctgacaaac tcggaacttg tttattgcag
     1501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1561 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
     1621 acaaagtcaa agcggccatc agatctgccg gtctccctat agtgagtcgt attaatttcg
     1681 ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2101 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     2161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2341 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3661 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3721 aaaaataggc gtatcacgag gcccttacgt attaattaa
//
