LOCUS       Exported                4289 bp ds-DNA     circular SYN 31-JAN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    FoxFTVC+FOG-MEK_S220E,S216D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, January 31, 2018 from SnapGene Viewer 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..303
                     /note="FoxF TVC"
                     /note="color: #800000"
     misc_feature    326..536
                     /note="bpFOG -1"
                     /note="color: #339966"
     misc_feature    545..1678
                     /note="MEK_S>E/S>D_MUT_ORF"
                     /note="color: #00ffff"
     misc_feature    1190..1192
                     /note="TCA>GAT_S>D Mutation"
                     /note="color: #a6acb3"
     misc_feature    1202..1204
                     /note="TCG>GAG_S>E Mutation"
                     /note="color: #a6acb3"
     polyA_signal    2015..2149
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(2186..2204)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(2462..3050)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(3221..4081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3221 .. 4012 / #ccffcc
                        2: 4013 .. 4081 / #ccffcc / signal sequence
                     Cleavage site after base 4012"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4082..4186)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggcgcgccgc gttgtatctt tcgttaagtg ctggcgtcga gcagggctaa cctcatttcg
       61 atttgacgat tcgggtaaaa ccgagcgatg gacaggacct ggaaatggga aaggcttact
      121 agaatcaggg tcgaacgaga taagaagatc aggattcgca aattagcact tggctttttt
      181 acgtagaaac ataggatgtc cgctgagccc cttatatata cgtagtacga atcaccggcc
      241 tggcgttgta taagcgagac cagcgtggac gaaaaggtaa ttgtttccga atggatcgcg
      301 ttctctagag gatccggcaa agcttcgtgt attgtaccgg cccattgtca atcatgcaaa
      361 cttgatatta tattgacaag agaagaaggc agtttaaatt aaaactctaa agtagagaga
      421 cattaatctc agctgacaag gcaggtggtc acagtaagtt catttaaata gttggccaac
      481 aatagccttt ccaagaaagt atttttgttc caggtctata caaaaataac acacaagcgg
      541 ccgcatgcct cctaaacgta agttaaaccc gttgaaccta acacttgagg ggagttcgcc
      601 tatccccaat cagcaggtct ctgaccttgg caagaagatg gacgaactaa acattgacga
      661 aactcaaagg aaaagattgg accagttcat taagcagaag caaaaagtgg gagtcatgga
      721 aaatgctcag aactctgact tcacaaagaa aggagaactg ggtgccggaa atgggggtgt
      781 tgttcatctt gtggtgcaca acgcaactgg ctttgtcatg gcgcggaaac ttattcattt
      841 ggaagtgaag caagccatct tgaaccaaat cacacgagaa ttgcaggttt tgcatgattg
      901 tagaagcccg tacattgttg ggtattatgg gactttttac agtgatgggg aaatcagtat
      961 ttgtatggaa agcatggacg ctgggtcgct tgatttagtg ctgaagaagg cgcgaaaaat
     1021 tcctgagatt tacttgggaa aagtgagcaa agctgttatc cttggcctca agtacttaag
     1081 agaagaacgt agcatcattc atcgcgatgt aaaaccatca aatattctcg ttaattcccg
     1141 aggagagatt aagttatgtg attttggcgt gagcgggcaa ctgatcgacg atatggccaa
     1201 cgagtttgta gggacaagat catacatggc cccagaacgc ttgcaaggat ccaagtatac
     1261 aatcctttca gatatctggt ctcttggtct ctcacttatt gaaatggcaa ttggtagatt
     1321 ccctatcccg ccacccacag ccagccaaat agcagccata ttcaacactg aagtggcagg
     1381 gggaagtggt aaagcaccaa acccacatga tgttgcacga ccaatggcaa tctttgagct
     1441 gcttgattac attgtgaatg agccagcacc gaaactccca caaggaattt tcgagaaaga
     1501 tttttgtgat ttcgtggcga gttgcttgaa gaaagaaccg aaagagcgat cagatctcgg
     1561 ggaactaatg aaggctccat ttattaaaaa tgttagctta acccagtatg agtttgctaa
     1621 gtgggtttgc agtactatgg gtttgaaagc cccgagtcca gacactgtac ctgattaact
     1681 ccacctagag gccagaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt
     1741 gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
     1801 ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact
     1861 tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata
     1921 tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc
     1981 aaccgctgtt tggtctgctt tctgacaaac tcggaacttg tttattgcag cttataatgg
     2041 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2101 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg acaaagtcaa
     2161 agcggccatc agatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg
     2221 ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2281 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2341 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2401 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2461 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2521 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2581 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2641 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2701 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2761 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2821 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2881 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2941 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3001 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3061 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3121 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3181 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3241 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3301 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3361 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3421 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3481 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3541 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3601 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3661 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3721 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3781 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3841 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3901 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3961 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4021 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4081 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4141 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4201 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4261 gtatcacgag gcccttacgt attaattaa
//
