LOCUS       Exported                3632 bp ds-DNA     circular SYN 31-JAN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    FoxFTVC+bpFOG_Cdkn1b.a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, February 1, 2018 from SnapGene Viewer 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..303
                     /note="FoxF TVC"
                     /note="color: #800000"
     misc_feature    326..536
                     /note="bpFOG -1"
                     /note="color: #339966"
     misc_feature    549..1037
                     /note="Cdkn1b.a_ORF"
                     /note="color: #00ffff"
     polyA_signal    1358..1492
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(1529..1547)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(1805..2393)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(2564..3424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2564 .. 3355 / #ccffcc
                        2: 3356 .. 3424 / #ccffcc / signal sequence
                     Cleavage site after base 3355"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3425..3529)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ggcgcgccgc gttgtatctt tcgttaagtg ctggcgtcga gcagggctaa cctcatttcg
       61 atttgacgat tcgggtaaaa ccgagcgatg gacaggacct ggaaatggga aaggcttact
      121 agaatcaggg tcgaacgaga taagaagatc aggattcgca aattagcact tggctttttt
      181 acgtagaaac ataggatgtc cgctgagccc cttatatata cgtagtacga atcaccggcc
      241 tggcgttgta taagcgagac cagcgtggac gaaaaggtaa ttgtttccga atggatcgcg
      301 ttctctagag gatccggcaa agcttcgtgt attgtaccgg cccattgtca atcatgcaaa
      361 cttgatatta tattgacaag agaagaaggc agtttaaatt aaaactctaa agtagagaga
      421 cattaatctc agctgacaag gcaggtggtc acagtaagtt catttaaata gttggccaac
      481 aatagccttt ccaagaaagt atttttgttc caggtctata caaaaataac acacaagcgg
      541 ccgcaaccat ggcggacaaa aaacccccgg ttcgacagct ccaaaaactc ccaggagtgc
      601 ggtctgcgcg gagaaattta tttggtcgag atgatagaat tgtggacgat ttagaaagag
      661 acgctgaaga gttgaacagt aaagaacaaa aacgattctc ggacacctgg aactttgatg
      721 cagccaatac ccaaccatta gatggcagat ttgaatggga caccggcgtt ccgctgaatc
      781 aacgaactcg actattgcat gttcccccga ttggtaatgc agattcacat aacgatagtg
      841 agcttgtaga agttaacaac tcaacagaat caaaaaatga agagttgcca cctacgggga
      901 attcctcttc tgtgggctca tgtggatctt cggaaaatca aagtaaaaga aggaaactat
      961 cttcagaatc aactgacgac catgtacccg ctacatcgaa gtcgccccga ctgtgacgtc
     1021 atactgtgcc acggaatgaa ttccagctga gcgccggtcg ctaccattac cagttggtct
     1081 ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat
     1141 ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt
     1201 acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat gacatcaacc
     1261 atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat
     1321 tccaaccgct gtttggtctg ctttctgaca aactcggaac ttgtttattg cagcttataa
     1381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgacaaagt
     1501 caaagcggcc atcagatctg ccggtctccc tatagtgagt cgtattaatt tcgataagcc
     1561 aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1981 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     2041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2221 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3541 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3601 ggcgtatcac gaggccctta cgtattaatt aa
//
