LOCUS       pX-hSpCas9    8090 bp      DNA     circular   02/02/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     rep_origin      7419..8047
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5573..6013)
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      5568..6023
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        6340..6368
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    910..933
                     /label=SV40 NLS1
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2179..3090
                     /label=REC3
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3274..3675
                     /label=HNH
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4246..5055
                     /label=PI
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    835..903
                     /label=3XFLAG
                     /SerialCloner_Color=&h107F01
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MDYKDHDGDYKDHDIDYKDDDDK" \ 
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattGtgcc 
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 
      301 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 
      361 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 
      421 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 
      481 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 
      541 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg 
      601 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 
      661 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 
      721 ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa 
      781 ttacctggag cacctgcctg aaatcacttt ttttcaggtt GGaccggtgc caccATGGAC 
      841 TATAAGGACC ACGACGGAGA CTACAAGGAT CATGATATTG ATTACAAAGA CGATGACGAT 
      901 AAGATGGCCC CAAAGAAGAA GCGGAAGGTC GGTATCCACG GAGTCCCAGC AGCCGACAAG 
      961 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC 
     1021 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC 
     1081 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG 
     1141 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA 
     1201 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG 
     1261 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG 
     1321 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG 
     1381 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG 
     1441 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG 
     1501 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC 
     1561 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA 
     1621 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC 
     1681 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA 
     1741 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC 
     1801 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC 
     1861 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG 
     1921 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG 
     1981 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT 
     2041 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG 
     2101 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTGCG GAAGCAGCGG 
     2161 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG 
     2221 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC 
     2281 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC 
     2341 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC 
     2401 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGgTGACCA ACTTCGATAA GAACCTGCCC 
     2461 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGaACGAGT ACTTCACCGT GTATAACGAG 
     2521 CTGACCgAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG 
     2581 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG 
     2641 CTGAAAGAGG ACTACTTCAA GAAAATCGAG TGCTTCGACT CCGTGGAAAT CTCCGGCGTG 
     2701 GAAGATCGGT TCAACGCCTC CCTGGGCACA TACCACGATC TGCTGAAAAT TATCAAGGAC 
     2761 AAGGACTTCC TGGACAATGA GGAAAACGAG GACATTCTGG AAGATATCGT GCTGACCCTG 
     2821 ACACTGTTTG AGGACAGAGA GATGATCGAG GAACGGCTGA AAACCTATGC CCACCTGTTC 
     2881 GACGACAAAG TGATGAAGCA GCTGAAGCGG CGGAGATACA CCGGCTGGGG CcaGCTGAGC 
     2941 CGGAAGCTGA TCAACGGCAT CCGGGACAAG CAGTCCGGCA AGACAATCCT GGATTTCCTG 
     3001 AAGTCCGACG GCTTCGCCAA CAGAAACTTC ATGCAGCTGA TCCACGACGA CAGCCTGACC 
     3061 TTTAAAGAGG ACATCCAGAA AGCCCAGGTG TCCGGCCAGG GCGATAGCCT GCACGAGCAC 
     3121 ATTGCCAATC TGGCCGGCAG CCCCGCCATT AAGAAGGGCA TCCTGCAGAC AGTGAAGGTG 
     3181 GTGGACGAGC TCGTGAAAGT GATGGGCCGG CACAAGCCCG AGAACATCGT GATCGAAATG 
     3241 GCCAGAGAGA ACCAGACCAC CCAGAAGGGA CAGAAGAACA GCCGCGAGAG AATGAAGCGG 
     3301 ATCGAAGAGG GCATCAAAGA GCTGGGCAGC CAGATCCTGA AAGAACACCC CGTGGAAAAC 
     3361 ACCCAGCTGC AGAACGAGAA GCTGTACCTG TACTACCTGC AGAATGGGCG GGATATGTAC 
     3421 GTGGACCAGG AACTGGACAT CAACCGGCTG TCCGACTACG ATGTGGACCA TATCGTGCCT 
     3481 CAGAGCTTTC TGAAGGACGA CTCCATCGAC AACAAGGTGC TGACCAGAAG CGACAAGAAC 
     3541 CGGGGCAAGA GCGACAACGT GCCCTCCGAA GAGGTCGTGA AGAAGATGAA GAACTACTGG 
     3601 CGGCAGCTGC TGAACGCCAA GCTGATTACC CAGAGAAAGT TCGACAATCT GACCAAGGCC 
     3661 GAGAGAGGCG GCCTGAGCGA ACTGGATAAG GCCGGCTTCA TCAAGAGACA GCTGGTGGAA 
     3721 ACCCGGCAGA TCACAAAGCA CGTGGCACAG ATCCTGGACT CCCGGATGAA CACTAAGTAC 
     3781 GACGAGAATG ACAAGCTGAT CCGGGAAGTG AAAGTGATCA CCCTGAAGTC CAAGCTGGTG 
     3841 TCCGATTTCC GGAAGGATTT CCAGTTTTAC AAAGTGCGCG AGATCAACAA CTACCACCAC 
     3901 GCCCACGACG CCTACCTGAA CGCCGTCGTG GGAACCGCCC TGATCAAAAA GTACCCTAAG 
     3961 CTGGAAAGCG AGTTCGTGTA CGGCGACTAC AAGGTGTACG ACGTGCGGAA GATGATCGCC 
     4021 AAGAGCGAGC AGGAAATCGG CAAGGCTACC GCCAAGTACT TCTTCTACAG CAACATCATG 
     4081 AACTTTTTCA AGACCGAGAT TACCCTGGCC AACGGCGAGA TCCGGAAGCG GCCTCTGATC 
     4141 GAGACAAACG GCGAAACCGG GGAGATCGTG TGGGATAAGG GCCGGGATTT TGCCACCGTG 
     4201 CGGAAAGTGC TGAGCATGCC CCAAGTGAAT ATCGTGAAAA AGACCGAGGT GCAGACAGGC 
     4261 GGCTTCAGCA AAGAGTCTAT CCTGCCCAAG AGGAACAGCG ATAAGCTGAT CGCCAGAAAG 
     4321 AAGGACTGGG ACCCTAAGAA GTACGGCGGC TTCGACAGCC CCACCGTGGC CTATTCTGTG 
     4381 CTGGTGGTGG CCAAAGTGGA AAAGGGCAAG TCCAAGAAAC TGAAGAGTGT GAAAGAGCTG 
     4441 CTGGGGATCA CCATCATGGA AAGAAGCAGC TTCGAGAAGA ATCCCATCGA CTTTCTGGAA 
     4501 GCCAAGGGCT ACAAAGAAGT GAAAAAGGAC CTGATCATCA AGCTGCCTAA GTACTCCCTG 
     4561 TTCGAGCTGG AAAACGGCCG GAAGAGAATG CTGGCCTCTG CCGGCGAACT GCAGAAGGGA 
     4621 AACGAACTGG CCCTGCCCTC CAAATATGTG AACTTCCTGT ACCTGGCCAG CCACTATGAG 
     4681 AAGCTGAAGG GCTCCCCCGA GGATAATGAG CAGAAACAGC TGTTTGTGGA ACAGCACAAG 
     4741 CACTACCTGG ACGAGATCAT CGAGCAGATC AGCGAGTTCT CCAAGAGAGT GATCCTGGCC 
     4801 GACGCTAATC TGGACAAAGT GCTGTCCGCC TACAACAAGC ACCGGGATAA GCCCATCAGA 
     4861 GAGCAGGCCG AGAATATCAT CCACCTGTTT ACCCTGACCA ATCTGGGAGC CCCTGCCGCC 
     4921 TTCAAGTACT TTGACACCAC CATCGACCGG AAGAGGTACA CCAGCACCAA AGAGGTGCTG 
     4981 GACGCCACCC TGATCCACCA GAGCATCACC GGCCTGTACG AGACACGGAT CGACCTGTCT 
     5041 CAGCTGGGAG GCGACAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA 
     5101 AAGtaagaat tcCTAGAGCT CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT 
     5161 CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC 
     5221 TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG 
     5281 GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGAgAATAGC AGGCATGCTG 
     5341 GGGAgcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct 
     5401 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct 
     5461 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta 
     5521 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag 
     5581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     5641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     5701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     5761 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata 
     5821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     5881 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc 
     5941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     6001 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc 
     6061 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 
     6121 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 
     6181 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt 
     6241 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 
     6301 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     6361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     6421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     6481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
     6541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
     6601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
     6661 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
     6721 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     6781 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     6841 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     6901 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     6961 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     7021 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     7081 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat 
     7141 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     7201 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     7261 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     7321 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     7381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     7441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     7501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     7561 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 
     7621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     7681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     7741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     7801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     7861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     7921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     7981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     8041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
//