LOCUS       hUBC_PP7_mCherry        5082 bp    DNA     circular UNA 20-APR-2018
DEFINITION  Concatenation of 2 sequences (Circularized).
ACCESSION   urn.local...1c-8x93q6j
VERSION     urn.local...1c-8x93q6j
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gene            complement(1..861)
                     /gene="bla"
                     /label="bla gene"
     CDS             complement(1..861)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="bla CDS"
     misc_feature    552..557
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, AssI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    590..601
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     misc_feature    669..678
                     /Recognition_pattern="GAANN^NNTTC"
                     /annotation_group="GAANNNNTTC"
                     /label="XmnI, Asp700I, MroXI, PdmI"
                     /note="Geneious type: restriction site"
     sig_peptide     complement(793..861)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
                     /label="bla signal peptide"
     misc_feature    876..881
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     -10_signal      complement(905..910)
                     /note="bla promoter (counter-clockwise); GAGACA;
                     transcript start 2548 (complementary strand)"
                     /label="-10 signal"
     -35_signal      complement(926..931)
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /label="-35 signal"
     misc_feature    1050^1051
                     /Original_Bases="GCCCTTTGCGCGTTTCGGTGATGACGGTGAAAACCTCTGAC
                     ACATGCAGCTCCCGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCG
                     TCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAA"
                     /label="GCCCTTTGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGC
                     TCCCGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGC
                     GTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAA"
                     /note="Geneious type: Editing History Deletion"
     source          order(<1051..>1316,<3878..>1050)
                     /organism="Cloning vector pNEB193"
                     /mol_type="other DNA"
                     /label="source Cloning vector pNEB193"
     misc_feature    1084..1094
                     /Recognition_pattern="GCANNNN^NTGC"
                     /annotation_group="GCANNNNNTGC"
                     /label="BstAPI"
                     /note="Geneious type: restriction site"
     misc_feature    1088..1093
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     primer_bind     1257..1280
                     /created_by="User"
                     /label="M13F (-47) Primer from GeneWiz"
     primer_bind     1283..1300
                     /created_by="User"
                     /label="M13F(-21) Primer From GeneWiz"
     misc_feature    1301..1306
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    1307..1312
                     /Recognition_pattern="GAG^CTC"
                     /annotation_group="GAGCTC"
                     /label="Ecl136II, Eco53kI, EcoICRI"
                     /note="Geneious type: restriction site"
     misc_feature    1307..1312
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, Psp124BI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    1331..1335
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1336..2539
                     /created_by="User"
                     /modified_by="User"
                     /label="human_UBC_promoter"
     misc_feature    1356..1361
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI, SstII"
                     /note="Geneious type: restriction site"
     misc_feature    1382..1387
                     /Recognition_pattern="AGC^GCT"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    1411
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1417^1418
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(1438..1444)
                     /Recognition_pattern="CCTNAGC(-5/-2)"
                     /annotation_group="CCTNAGC"
                     /label="Bpu10I"
                     /note="Geneious type: restriction site"
     misc_feature    1480..1490
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    2546..2551
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    2546..2551
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    2558..2567
                     /created_by="User"
                     /modified_by="User"
                     /label="Kozak_Sequence"
     sig_peptide     2564..2590
                     /created_by="User"
                     /label="NLS"
     CDS             2564..3745
                     /created_by="User"
                     /modified_by="User"
                     /label
     misc_feature    2597..2623
                     /created_by="User"
                     /label="HA_Tag"
     misc_feature    2630..2998
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="humanized PP7 (DeltaFG)"
     misc_feature    2764..2770
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    2798..2803
                     /Recognition_pattern="GTT^AAC"
                     /annotation_group="GTTAAC"
                     /label="HpaI, KspAI"
                     /note="Geneious type: restriction site"
     misc_feature    2909..2915
                     /Recognition_pattern="T^CCNGGA"
                     /annotation_group="TCCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     misc_feature    2914..2926
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    2932..2940
                     /Recognition_pattern="GACN^NNGTC"
                     /annotation_group="GACNNNGTC"
                     /label="Tth111I, AspI, PflFI, PsyI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2999..3004)
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI site"
     misc_feature    2999..3004
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    3005..3028
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="10 aa linker"
     misc_feature    3029..3034
                     /created_by="User"
                     /label="BspEI site"
     misc_feature    3029..3034
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     CDS             3035..3742
                     /created_by="User"
                     /label="mCherry"
     misc_feature    3063..3068
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI, MlsI, MluNI, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    3299..3309
                     /Recognition_pattern="(8/13)AAGNNNNNCTT(13/8)"
                     /annotation_group="AAGNNNNNCTT"
                     /label="FalI"
                     /note="Geneious type: restriction site"
     misc_feature    3468..3478
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI, AccB7I, BasI, Van91I"
                     /note="Geneious type: restriction site"
     misc_feature    3479..3489
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     misc_feature    3608..3613
                     /Recognition_pattern="GTGCAG(16/14)"
                     /annotation_group="GTGCAG"
                     /label="BsgI"
                     /note="Geneious type: restriction site"
     misc_feature    3715..3722
                     /Recognition_pattern="CR^CCGGYG"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    3735..3740
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    3743..3745
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="STOP"
     misc_feature    3752..3757
                     /created_by="User"
                     /label="SalI Site"
     misc_feature    3752..3757
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    3752..3757
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI, FblI, XmiI"
                     /note="Geneious type: restriction site"
     terminator      3758..3877
                     /label="SV40_PA_terminator"
     source          <3758..>3877
                     /organism="pcDNA3.1(+)"
                     /mol_type="other DNA"
                     /label="source pcDNA3.1(+)"
     misc_feature    complement(3792..3811)
                     /created_by="User"
                     /modified_by="User"
                     /label="SV40pA Rev Primer from Genewiz"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3835..3840)
                     /Recognition_pattern="GAATGC(1/-1)"
                     /annotation_group="GAATGC"
                     /label="BsmI, BsaMI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     primer_bind     3846..3865
                     /modified_by="User"
                     /label="EBV_rev_primer"
     misc_feature    3884..3891
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     misc_feature    3898..3903
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI, BbuI, PaeI"
                     /note="Geneious type: restriction site"
     misc_feature    3904..3909
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3922..3938)
                     /created_by="User"
                     /label="M13R Primer From GeneWiz"
                     /note="Geneious type: primer_bind_reverse"
     -10_signal      complement(3971..3976)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
                     /label="-10 signal"
     -35_signal      complement(3995..4000)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
                     /label="-35 signal"
     protein_bind    complement(4020..4032)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
                     /label="CAP protein binding site protein bind"
     misc_feature    4140..4146
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    4263..4268
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     rep_origin      complement(4324..4912)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label="rep origin"
     misc_RNA        complement(4324..4876)
                     /note="RNAII transcript (complementary strand)"
                     /label="RNA"
     -35_signal      4730..4735
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label="RNAI promoter (clockwise); TTGAAG -35 signal"
     -10_signal      4752..4757
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label="RNAI promoter (clockwise); GCTACA -10 signal"
     misc_RNA        4766..4873
                     /note="RNAI transcript"
                     /label="RNAI transcript"
     -10_signal      complement(4886..4891)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label="-10 signal"
     -35_signal      complement(4907..4912)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label="-35 signal"
ORIGIN      
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag ctatgcggca tcagagcaga ttgtactgag
     1081 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1141 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1201 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1261 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacacg
     1321 tatgtcgagg taggcgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     1381 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     1441 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     1501 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     1561 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     1621 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     1681 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     1741 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     1801 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     1861 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     1921 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     1981 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     2041 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     2101 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     2161 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     2221 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     2281 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     2341 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     2401 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     2461 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     2521 ttttggcttt tttgttagac ctggaggtac cgccaccgcc accatggctc caaagaagaa
     2581 gcgtaaggta ggcagctacc catacgatgt tccagattac gctggcagct ccatgtcaaa
     2641 gacgatcgtg ctgtctgtag gtgaagccac acgcaccctc actgagatac agtccacggc
     2701 cgataggcag atttttgagg agaaggtggg tccactggtg ggtaggcttc gactgacggc
     2761 ctccctgagg caaaacggcg ctaaaacggc ttatcgcgtt aacctcaagc tggaccaggc
     2821 cgatgtcgtt gatagtggac tgcccaaagt caggtacaca caggtgtgga gccacgatgt
     2881 gaccatcgtg gctaactcaa cagaggcgtc ccggaagtct ctgtacgacc tgacaaagtc
     2941 cctggttgcc acctctcagg tggaggatct ggtggttaat ctcgtgcccc tcggacgggg
     3001 atccaagggc tcgggctcga cctcgggctc cggaatggtg agcaagggcg aggaggacaa
     3061 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3121 ccgcgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3181 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca
     3241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     3301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     3361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     3421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     3481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     3541 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acaaggccaa
     3601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca tcacctccca
     3661 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg
     3721 cggcatggac gagctgtaca agtaatgata agtcgacttg tttattgcag cttataatgg
     3781 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3841 tagttgtggt ttgtccaaac tcatcaatgt atcttattcg actgtttaaa cctgcaggca
     3901 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3961 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4021 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4081 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4741 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5041 taaatcaatc taaagtatat atgagtaaac ttggtctgac ag
//
