LOCUS       pJJB296                 6626 bp ds-DNA     circular     25-MAY-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1187..1724
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             5833..6492
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1740..5195
                     /label="LwCas13a"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      62..690
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     polyA_site      5204..5453
                     /label="HSP polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 acgtgccttg acggcaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctactaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgctca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg aagagcgccc acctgctcat gatcggcgcg ccagatttgc cttttcaatt
      901 tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcaggt atcatcaaga
      961 cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag gttaaagatg
     1021 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga
     1081 tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca aggcaagtaa
     1141 tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg gagtcaaaga
     1201 ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc
     1261 tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg
     1321 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
     1381 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
     1441 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
     1501 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     1561 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     1621 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     1681 ctatataagg aagttcattt catttggaga gaacacgggg gactccCGAC ATGGGATCCA
     1741 TGAAAGTGAC CAAGGTCGAC GGCATCAGCC ACAAGAAGTA CATCGAAGAG GGCAAGCTCG
     1801 TGAAGTCCAC CAGCGAGGAA AACCGGACCA GCGAGAGACT GAGCGAGCTG CTGAGCATCC
     1861 GGCTGGACAT CTACATCAAG AACCCCGACA ACGCCTCCGA GGAAGAGAAC CGGATCAGAA
     1921 GAGAGAACCT GAAGAAGTTC TTTAGCAACA AGGTGCTGCA CCTGAAGGAC AGCGTGCTGT
     1981 ATCTGAAGAA CCGGAAAGAA AAGAACGCCG TGCAGGACAA GAACTATAGC GAAGAGGACA
     2041 TCAGCGAGTA CGACCTGAAA AACAAGAACA GCTTCTCCGT GCTGAAGAAG ATCCTGCTGA
     2101 ACGAGGACGT GAACTCTGAG GAACTGGAAA TCTTTCGGAA GGACGTGGAA GCCAAGCTGA
     2161 ACAAGATCAA CAGCCTGAAG TACAGCTTCG AAGAGAACAA GGCCAACTAC CAGAAGATCA
     2221 ACGAGAACAA CGTGGAAAAA GTGGGCGGCA AGAGCAAGCG GAACATCATC TACGACTACT
     2281 ACAGAGAGAG CGCCAAGCGC AACGACTACA TCAACAACGT GCAGGAAGCC TTCGACAAGC
     2341 TGTATAAGAA AGAGGATATC GAGAAACTGT TTTTCCTGAT CGAGAACAGC AAGAAGCACG
     2401 AGAAGTACAA GATCCGCGAG TACTATCACA AGATCATCGG CCGGAAGAAC GACAAAGAGA
     2461 ACTTCGCCAA GATTATCTAC GAAGAGATCC AGAACGTGAA CAACATCAAA GAGCTGATTG
     2521 AGAAGATCCC CGACATGTCT GAGCTGAAGA AAAGCCAGGT GTTCTACAAG TACTACCTGG
     2581 ACAAAGAGGA ACTGAACGAC AAGAATATTA AGTACGCCTT CTGCCACTTC GTGGAAATCG
     2641 AGATGTCCCA GCTGCTGAAA AACTACGTGT ACAAGCGGCT GAGCAACATC AGCAACGATA
     2701 AGATCAAGCG GATCTTCGAG TACCAGAATC TGAAAAAGCT GATCGAAAAC AAACTGCTGA
     2761 ACAAGCTGGA CACCTACGTG CGGAACTGCG GCAAGTACAA CTACTATCTG CAAGTGGGCG
     2821 AGATCGCCAC CTCCGACTTT ATCGCCCGGA ACCGGCAGAA CGAGGCCTTC CTGAGAAACA
     2881 TCATCGGCGT GTCCAGCGTG GCCTACTTCA GCCTGAGGAA CATCCTGGAA ACCGAGAACG
     2941 AGAACGGTAT CACCGGCCGG ATGCGGGGCA AGACCGTGAA GAACAACAAG GGCGAAGAGA
     3001 AATACGTGTC CGGCGAGGTG GACAAGATCT ACAATGAGAA CAAGCAGAAC GAAGTGAAAG
     3061 AAAATCTGAA GATGTTCTAC AGCTACGACT TCAACATGGA CAACAAGAAC GAGATCGAGG
     3121 ACTTCTTCGC CAACATCGAC GAGGCCATCA GCAGCATCAG ACACGGCATC GTGCACTTCA
     3181 ACCTGGAACT GGAAGGCAAG GACATCTTCG CCTTCAAGAA TATCGCCCCC AGCGAGATCT
     3241 CCAAGAAGAT GTTTCAGAAC GAAATCAACG AAAAGAAGCT GAAGCTGAAA ATCTTCAAGC
     3301 AGCTGAACAG CGCCAACGTG TTCAACTACT ACGAGAAGGA TGTGATCATC AAGTACCTGA
     3361 AGAATACCAA GTTCAACTTC GTGAACAAAA ACATCCCCTT CGTGCCCAGC TTCACCAAGC
     3421 TGTACAACAA GATTGAGGAC CTGCGGAATA CCCTGAAGTT TTTTTGGAGC GTGCCCAAGG
     3481 ACAAAGAAGA GAAGGACGCC CAGATCTACC TGCTGAAGAA TATCTACTAC GGCGAGTTCC
     3541 TGAACAAGTT CGTGAAAAAC TCCAAGGTGT TCTTTAAGAT CACCAATGAA GTGATCAAGA
     3601 TTAACAAGCA GCGGAACCAG AAAACCGGCC ACTACAAGTA TCAGAAGTTC GAGAACATCG
     3661 AGAAAACCGT GCCCGTGGAA TACCTGGCCA TCATCCAGAG CAGAGAGATG ATCAACAACC
     3721 AGGACAAAGA GGAAAAGAAT ACCTACATCG ACTTTATTCA GCAGATTTTC CTGAAGGGCT
     3781 TCATCGACTA CCTGAACAAG AACAATCTGA AGTATATCGA GAGCAACAAC AACAATGACA
     3841 ACAACGACAT CTTCTCCAAG ATCAAGATCA AAAAGGATAA CAAAGAGAAG TACGACAAGA
     3901 TCCTGAAGAA CTATGAGAAG CACAATCGGA ACAAAGAAAT CCCTCACGAG ATCAATGAGT
     3961 TCGTGCGCGA GATCAAGCTG GGGAAGATTC TGAAGTACAC CGAGAATCTG AACATGTTTT
     4021 ACCTGATCCT GAAGCTGCTG AACCACAAAG AGCTGACCAA CCTGAAGGGC AGCCTGGAAA
     4081 AGTACCAGTC CGCCAACAAA GAAGAAACCT TCAGCGACGA GTTGGAACTG ATCAACCTGC
     4141 TGAACCTGGA CAACAACAGA GTGACCGAGG ACTTCGAGCT GGAAGCCAAC GAGATCGGCA
     4201 AGTTCCTGGA CTTCAACGAA AACAAAATCA AGGACCGGAA AGAGCTGAAA AAGTTCGACA
     4261 CCAACAAGAT CTATTTCGAC GGCGAGAACA TCATCAAGCA CCGGGCCTTC TACAATATCA
     4321 AGAAATACGG CATGCTGAAT CTGCTGGAAA AGATCGCCGA TAAGGCCAAG TATAAGATCA
     4381 GCCTGAAAGA ACTGAAAGAG TACAGCAACA AGAAGAATGA GATTGAAAAG AACTACACCA
     4441 TGCAGCAGAA CCTGCACCGG AAGTACGCCA GACCCAAGAA GGACGAAAAG TTCAACGACG
     4501 AGGACTACAA AGAGTATGAG AAGGCCATCG GCAACATCCA GAAGTACACC CACCTGAAGA
     4561 ACAAGGTGGA ATTCAATGAG CTGAACCTGC TGCAGGGCCT GCTGCTGAAG ATCCTGCACC
     4621 GGCTCGTGGG CTACACCAGC ATCTGGGAGC GGGACCTGAG ATTCCGGCTG AAGGGCGAGT
     4681 TTCCCGAGAA CCACTACATC GAGGAAATTT TCAATTTCGA CAACTCCAAG AATGTGAAGT
     4741 ACAAAAGCGG CCAGATCGTG GAAAAGTATA TCAACTTCTA CAAAGAACTG TACAAGGACA
     4801 ATGTGGAAAA GCGGAGCATC TACTCCGACA AGAAAGTGAA GAAACTGAAG CAGGAAAAAA
     4861 AGGACCTGTA CATCCGGAAC TACATTGCCC ACTTCAACTA CATCCCCCAC GCCGAGATTA
     4921 GCCTGCTGGA AGTGCTGGAA AACCTGCGGA AGCTGCTGTC CTACGACCGG AAGCTGAAGA
     4981 ACGCCATCAT GAAGTCCATC GTGGACATTC TGAAAGAATA CGGCTTCGTG GCCACCTTCA
     5041 AGATCGGCGC TGACAAGAAG ATCGAAATCC AGACCCTGGA ATCAGAGAAG ATCGTGCACC
     5101 TGAAGAATCT GAAGAAAAAG AAACTGATGA CCGACCGGAA CAGCGAGGAA CTGTGCGAAC
     5161 TCGTGAAAGT CATGTTCGAG TACAAGGCCC TGGAATGAcg acgatatgaa gatgaagatg
     5221 aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct
     5281 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa
     5341 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
     5401 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aagccagagc
     5461 tctggtgacg gacgtgggca ggtgtgatga aaagccttag ggtcaggtgg cacttttcgg
     5521 ggaaatgtgc gatgaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5581 ctaatgagac aataaccctg ataaatgctt caattatatt gaaaaaggaa gagtatgagt
     5641 atccaacatt tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct tcctgttttt
     5701 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg
     5761 ggttacattg aactggatct caacagcggc aaaatcttag agagttttcg ccccgaagaa
     5821 cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt
     5881 gatgctgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5941 tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgtagt
     6001 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga
     6061 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6121 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6181 gcaatggcaa caactctccg caaactatta actggcgaac tacttactct tgcttcacgc
     6241 cagcaactca ttgactggat ggaggcggac aaagttgcag gaccacttct gcgctcggca
     6301 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6361 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6421 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6481 attaagcatt ggtaactgtc agacgaaggg aaataaatag tagcccgcct gatgtgcggg
     6541 cttttttttg gtggtggtta ccagtgtcgc ttagctaccg taggtcctct taggcggtcc
     6601 gattagcgac atagtcatca attgca
//