LOCUS Exported 5756 bp ds-DNA circular SYN 30-MAR-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5756) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 30, 2018 from SnapGene 4.1.7 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5756 /organism="synthetic DNA construct" /note="/dnas_title=EB1-N-LZ-mCherry-LOV2 wt" misc_feature 600..1154 /label=EB1 N terminus /note="EB1 N terminus" /note="/dnas_title=EB1N (AA 1-185)" misc_feature 867..885 /label=shRNA#3 resistant (Addgene #37927) /note="shRNA#3 resistant (Addgene #37927)" /note="/dnas_title=shRNA#3 resistant (Addgene #37927)" misc_feature 1155..1247 /label=GCN4 Leucine Zipper /note="GCN4 Leucine Zipper" misc_feature 1254..1961 /label=mCherry /note="mCherry" misc_feature 1983..2414 /label=LOV2 wt /note="LOV2 wt" /note="/dnas_title=LOV2(wt)" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca 601 tggcagtgaa cgtatactca acgtcagtga ccagtgataa cctaagtcga catgacatgc 661 tggcctggat caatgagtct ctgcagttga atctgacaaa gatcgaacag ttgtgctcag 721 gggctgcgta ttgtcagttt atggacatgc tgttccctgg ctccattgcc ttgaagaaag 781 tgaaattcca agctaagcta gaacacgagt acatccagaa cttcaaaata ttacaagcag 841 gttttaagag aatgggtgtt gacaaaataa tcccggtgga caaattagta aaaggaaagt 901 ttcaggacaa ttttgaattc gttcagtggt tcaagaagtt cttcgatgca aactatgatg 961 gaaaagacta tgaccctgtg gctgctagac aaggtcaaga aactgcagtg gctccttccc 1021 ttgttgctcc agctctgaat aaaccgaaga aacctctcac ttctagcagt gcagctcccc 1081 agaggcccat ctcaacacag agaaccgctg cggctcctaa ggctggccct ggtgtggtgc 1141 gaaagaaccc tggtatgaaa cagctggaag ataaagtgga agagctgttg agcaaaaact 1201 atcatctgga aaacgaagtg gcgcgtctga aaaagctggt gggcgaactc gagatggtga 1261 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 1321 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 1381 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 1441 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 1501 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 1561 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 1621 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggccct gtaatgcaga 1681 agaagactat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 1741 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 1801 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 1861 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 1921 agggccgcca ctccaccggc ggcatggacg agctgtacaa gccgcggggt tctggaagtg 1981 gattggctgc tgcacttgaa cgtattgaga agaactttgt cattactgac ccaagattgc 2041 cagataatcc cattatattc gcgtccgata gtttcttgca gttgacagaa tatagccgtg 2101 aagaaatttt gggaagaaac tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag 2161 tgagaaaaat tagagatgcc atagataacc aaacagaggt cactgttcag ctgattaatt 2221 atacaaagag tggtaaaaag ttctggaacc tctttcactt gcagcctatg cgagatcaga 2281 agggagatgt ccagtacttt attggggttc agttggatgg aactgagcat gtccgagatg 2341 ctgccgagag agagggagtc atgctgatta agaaaactgc agaaaatatt gatgaggcgg 2401 caaaagaact ttaaggatcc accggatcta gataactgat cataatcagc cataccacat 2461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata 2521 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa 2581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 2641 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa 2701 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa 2761 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac 2821 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag 2881 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt 2941 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg 3001 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca 3061 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag 3121 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 3181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 3241 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag 3301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3361 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3421 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3481 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3541 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3601 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa 3661 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 3721 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 3781 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag 3841 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 3901 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 3961 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 4021 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 4081 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 4141 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat 4201 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 4261 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 4321 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 4381 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 4441 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 4501 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 4561 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta 4621 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg 4681 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc 4741 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc 4801 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag 4861 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg 4921 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 4981 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 5041 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5101 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5161 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 5221 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 5281 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 5341 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 5401 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 5461 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 5521 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 5581 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 5641 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 5701 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat //