LOCUS Exported 6817 bp ds-DNA circular SYN 01-AUG-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-sCAG-SoCoChR-GFP SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 6817) AUTHORS Changyang Linghu TITLE Direct Submission JOURNAL Exported Friday, Apr 7, 2017 from SnapGene 3.2.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6817 /organism="recombinant plasmid" /mol_type="other DNA" promoter 487..764 /note="chicken beta-actin promoter" CDS 1174..3270 /gene="ChR86-12-KA2-(1-150)-GFP" /note="ChR86-12-KA2-(1-150)-GFP" CDS 1186..2553 /codon_start=1 /note="SoCoChR" /note="No FP" /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD QEDEETVGGSGGTGGSGGTMPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGE RLALALAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSS PASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVSGASGGT" CDS 2050..2085 /codon_start=1 /note="Linker12" /translation="GGSGGTGGSGGT" CDS 2086..2535 /codon_start=1 /note="KA2(1-150)" /translation="MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALA LAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASAS TVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVS" CDS 2554..3270 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" misc_feature 3295..3883 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(3766..3777) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" polyA_signal 3926..4047 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 4515..5399 /note="pUC Origin" rep_origin complement(4575..5163) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5334..6194) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 5475 /note="Point mutation" promoter complement(6195..6299) /gene="bla" /note="AmpR promoter" rep_origin complement(6325..6780) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc 61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg 121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa 181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaaccat 241 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 301 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 361 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 421 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 481 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac 541 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg 601 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga 661 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc 721 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc 781 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 841 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 901 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc 961 cgggagggcc ctttgtgcgg ggggagcggc tcggggctgt ccgcgggggg acggctgcct 1021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc 1081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt 1141 tattgtgctg tctcatcatt ttggcaaaga attggatccg ccaccatgct ggggaacggc 1201 agcgccattg tgcctatcga ccagtgcttt tgcctggctt ggaccgacag cctgggaagc 1261 gatacagagc agctggtggc caacatcctc cagtggttcg ccttcggctt cagcatcctg 1321 atcctgatgt tctacgccta ccagacttgg agagccactt gcggttggga ggaggtctac 1381 gtctgttgcg tcgagctgac caaggtcatc atcgagttct tccacgagtt cgacgacccc 1441 agcatgctgt acctggctaa cggacaccga gtccagtggc tgagatacgc agagtggctg 1501 ctgacttgtc ccgtcatcct gatccacctg agcaacctga ccggcctgaa ggacgactac 1561 agcaagcgga ccatgaggct gctggtgtca gacgtgggaa ccatcgtgtg gggagctaca 1621 agcgccatga gcacaggcta cgtcaaggtc atcttcttcg tgctgggttg catctacggc 1681 gccaacacct tcttccacgc cgccaaggtg tatatcgaga gctaccacgt ggtgccaaag 1741 ggcagaccta gaaccgtcgt gcggatcatg gcttggctgt tcttcctgtc ttggggcatg 1801 ttccccgtgc tgttcgtcgt gggaccagaa ggattcgacg ccatcagcgt gtacggctct 1861 accattggcc acaccatcat cgacctcatg agcaagaatt gttggggcct gctgggacac 1921 tatctgagag tgctgatcca ccagcacatc atcatctacg gcgacatccg gaagaagacc 1981 aagatcaacg tggccggcga ggagatggaa gtggagacca tggtggacca ggaggacgag 2041 gagacagtgg gaggttcagg tggaaccggt ggaagtggag gtaccatgcc cgccgaactg 2101 ctgctgctgc tgattgtcgc ttttgctaac ccttcttgcc aggtgctgtc ttctctgagg 2161 atggccgcta ttctggacga tcagaccgtg tgcggaagag gagagaggct ggcactggca 2221 ctggctaggg agcagatcaa tggcatcatt gaagtgcctg caaaggcccg ggtcgaggtg 2281 gacattttcg aactgcagag agatagccag tacgagacca cagacaccat gtgccagatc 2341 ctgccaaaag gagtggtctc cgtcctggga ccaagctcct ctcctgcttc tgcaagtacc 2401 gtgtctcaca tttgtggcga gaaggaaatc ccccatatca aggtcgggcc agaggaaaca 2461 cccaggctgc agtacctgcg cttcgcctca gtgagcctgt atccatcaaa cgaggatgtg 2521 tcactggcag tcagcggagc tagcggaggt actgtgagca agggcgagga gctgttcacc 2581 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 2641 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catttgcacc 2701 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 2761 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 2821 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 2881 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 2941 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 3001 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 3061 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 3121 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 3181 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 3241 actctcggca tggacgagct gtacaagtaa gaattcgata tcaagcttat cgataatcaa 3301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 3361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 3421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 3481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 3541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 3601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 3661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 3721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 3781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 3841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgaccc 3901 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact 3961 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 4021 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 4081 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa 4141 atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat 4201 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct 4261 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt 4321 gagcgagcga gcgcgcagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 4801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 5041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 5101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 5161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 5221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 5281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5461 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 6001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 6061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 6121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 6181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 6241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6301 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt 6361 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 6421 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 6481 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 6541 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 6601 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 6661 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 6721 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 6781 cccattcgcc attcaggctg cgcaactgtt gggaagg //