LOCUS       GS59114-4-pAAV-Syn-FLEX-ChR86-12-KA2-(1-150)-GFP                6777 bp ds-DNA     circular SYN 14-Apr-2016
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    GS59114-4 pAAV-Syn-FLEX-ChR86-12-KA2-(1-150)-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   AY
  TITLE     Direct Submission
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3305..5389
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /note="Rep_Origin_1"
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pMB1 origin of replication 
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point); 
                     pUC19 origin of replication (clockwise) (RNAII -35 to 
                     RNA/DNA switch point); pUC19 origin of replication 
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point)"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     sig_peptide     1733..1801
                     /gene="bla"
                     /note="bla"
                     /note="Required for secretion to the periplasm; cleaved off
                     to form the mature beta-lactamase protein.; required for 
                     secretion to the periplasm; cleaved off to form the mature 
                     beta-lactamase protein; required for secretion to the 
                     periplasm; cleaved off to form the mature beta-lactamase 
                     protein."
     promoter        complement(1802..1906)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        complement(1843..1871)
                     /note="amp prom"
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     repeat_region   2458..2647
                     /note="ITR"
     promoter        2691..3138
                     /note="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    3154..3187
                     /note="Lox2272"
     protein_bind    3250..3283
                     /note="LoxP"
     CDS             complement(3305..4021)
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(4040..4489)
                     /codon_start=1
                     /note="KA2(1-150)"
                     /translation="MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALA
                     LAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASAS
                     TVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVS"
     CDS             complement(4490..4525)
                     /codon_start=1
                     /note="GGSGGTGGSGGT"
                     /translation="GGSGGTGGSGGT"
     CDS             complement(4526..5389)
                     /codon_start=1
                     /note="ChR86"
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     protein_bind    5417..5450
                     /note="Lox2272"
     protein_bind    5521..5554
                     /note="LoxP"
     misc_feature    5587..6175
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6058..6069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     polyA_signal    6216..6423
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6534..6723
                     /note="ITR"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa gtgtctagac tgcagagggc cctgcgtatg agtgcaagtg
     2701 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca
     2761 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
     2821 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
     2881 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
     2941 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
     3001 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
     3061 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
     3121 tcgtgcctga gagcgcagtc gagaagaatt cccataactt cgtataaagt atcctatacg
     3181 aagttatatc aaaataggaa gaccaatgct tcaccatcga cccgaattgc caagcatcac
     3241 catcgaccca taacttcgta taatgtatgc tatacgaagt tatactagct agcgtttaaa
     3301 ctcattactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     3361 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     3421 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     3481 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     3541 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     3601 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     3661 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     3721 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     3781 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     3841 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca aatgaacttc agggtcagct
     3901 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     3961 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     4021 cagtacctcc gctagctccg ctgactgcca gtgacacatc ctcgtttgat ggatacaggc
     4081 tcactgaggc gaagcgcagg tactgcagcc tgggtgtttc ctctggcccg accttgatat
     4141 gggggatttc cttctcgcca caaatgtgag acacggtact tgcagaagca ggagaggagc
     4201 ttggtcccag gacggagacc actccttttg gcaggatctg gcacatggtg tctgtggtct
     4261 cgtactggct atctctctgc agttcgaaaa tgtccacctc gacccgggcc tttgcaggca
     4321 cttcaatgat gccattgatc tgctccctag ccagtgccag tgccagcctc tctcctcttc
     4381 cgcacacggt ctgatcgtcc agaatagcgg ccatcctcag agaagacagc acctggcaag
     4441 aagggttagc aaaagcgaca atcagcagca gcagcagttc ggcgggcatg gtacctccac
     4501 ttccaccggt tccacctgaa cctcccactg tctcctcgtc ctcctggtcc accatggtct
     4561 ccacttccat ctcctcgccg gccacgttga tcttggtctt cttccggatg tcgccgtaga
     4621 tgatgatgtg ctggtggatc agcactctca gatagtgtcc cagcaggccc caacaattct
     4681 tgctcatgag gtcgatgatg gtgtggccaa tggtagagcc gtacacgctg atggcgtcga
     4741 atccttctgg tcccacgacg aacagcacgg ggaacatgcc ccaagacagg aagaacagcc
     4801 aagccatgat ccgcacgacg gttctaggtc tgccctttgg caccacgtgg tagctctcga
     4861 tatacacctt ggcggcgtgg aagaaggtgt tggcgccgta gatgcaaccc agcacgaaga
     4921 agatgacctt gacgtagcct gtgctcatgg cgcttgtagc tccccacacg atggttccca
     4981 cgtctgacac cagcagcctc atggtccgct tgctgtagtc gtccttcagg ccggtcaggt
     5041 tgctcaggtg gatcaggatg acgggacaag tcagcagcca ctctgcgtat ctcagccact
     5101 ggactcggtg tccgttagcc aggtacagca tgctggggtc gtcgaactcg tggaagaact
     5161 cgatgatgac cttggtcagc tcgacgcaac agacgtagac ctcctcccaa ccgcaagtgg
     5221 ctctccaagt ctggtaggcg tagaacatca ggatcaggat gctgaagccg aaggcgaacc
     5281 actggaggat gttggccacc agctgctctg tatcgcttcc caggctgtcg gtccaagcca
     5341 ggcaaaagca ctggtcgata ggcacaatgg cgctgccgtt ccccagcatg gtggcggcgc
     5401 ggcctaggta cctagtataa cttcgtatag gatactttat acgaagttat cattgggatt
     5461 cttcctattt tgatccaagc atcaccatcg accctctagt ccagatctca ccatcgaccc
     5521 ataacttcgt atagcataca ttatacgaag ttatgtccct cgaagaggtt cgcgaagctt
     5581 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5641 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5701 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5761 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5821 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5881 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5941 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6001 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6061 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6121 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     6181 taccgtcgac ccgggcggcc gctcataaag ctcgactgtg ccttctagtt gccagccatc
     6241 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6301 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6361 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6421 ggatgcggtc cggactgtac aattgcattc attttatgtt tcaggttcag ggggagatgt
     6481 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc
     6541 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     6601 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     6661 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     6721 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgct
//
