LOCUS       Exported               10778 bp ds-DNA     circular SYN 31-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   jinleleliu
  TITLE     Direct Submission
  JOURNAL   Exported Feb 15, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..400
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             420..440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             441..4541
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4554..4574
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4607..4854
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        5080..5423
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        5424..5471
                     /label=EM7 promoter
     CDS             5490..6062
                     /codon_start=1
                     /label=nat1
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      6068..6265
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6766..7569)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7570..7785)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     misc_feature    8055..8558
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8595..8699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8700..9560
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9731..10319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        10643..10673
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10681..10697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10705..10721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10742..10760
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc
       61 atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct
      121 ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt
      181 ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa
      241 tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac
      301 ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact
      361 tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaattac aaaatgcctc
      421 caaaaaagaa gagaaaggtc gacaagaagt actccattgg gctcgatatc ggcacaaaca
      481 gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc
      541 tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc ctgttcgact
      601 ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga tatacccgca
      661 gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct aaggtggatg
      721 actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa aagcacgagc
      781 gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag tacccaacca
      841 tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg cggttgatct
      901 atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag ggggacctga
      961 acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact tacaatcagc
     1021 ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc ctgagcgcta
     1081 ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga
     1141 acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac tttaaatcta
     1201 acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac gatgatgatc
     1261 tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg gcggcaaaga
     1321 acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag atcaccaaag
     1381 ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac ttgactttgc
     1441 tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc ttcgatcagt
     1501 ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa ttttacaaat
     1561 ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta aagcttaaca
     1621 gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc caccagattc
     1681 acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc tttttgaaag
     1741 ataacaggga aaagattgag aaaatcctca catttcggat accctactat gtaggccccc
     1801 tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag accatcactc
     1861 cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc atcgaaagga
     1921 tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt
     1981 acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca gaagggatga
     2041 gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc ctcttcaaga
     2101 cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag attgaatgtt
     2161 tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc
     2221 acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag aacgaggaca
     2281 ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg attgaagaac
     2341 gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc
     2401 gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga gacaagcaga
     2461 gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg aacttcatgc
     2521 agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca caagtttctg
     2581 gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca gctatcaaaa
     2641 agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata
     2701 agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag aagggacaga
     2761 agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg gggtcccaaa
     2821 tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc tacctgtact
     2881 acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat cggctctccg
     2941 actacgacgt ggatcatatc gtgccccagt cttttctcaa agatgattct attgataata
     3001 aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc tcagaagaag
     3061 ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg atcacacaac
     3121 ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg gataaagccg
     3181 gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg gcccaaattc
     3241 tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga gaggtgaaag
     3301 ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag ttttataagg
     3361 tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca gtggtaggca
     3421 ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga gactataaag
     3481 tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag gccaccgcta
     3541 agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca ctggccaatg
     3601 gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa atcgtgtggg
     3661 acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag gtgaacatcg
     3721 ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc ccgaaaagga
     3781 acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac ggcggattcg
     3841 attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa gggaagtcta
     3901 aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga tcaagcttcg
     3961 aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa aaagacctca
     4021 tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa cgaatgctcg
     4081 ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa tacgttaatt
     4141 tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga
     4201 agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag caaataagcg
     4261 aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt tctgcttaca
     4321 ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac ttgtttactc
     4381 tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata gacagaaagc
     4441 ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca attacggggc
     4501 tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct gaccccaaga
     4561 agaagaggaa ggtgtgattt tggacctcga gtcattggac ctcgagtcat gtaattagtt
     4621 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     4681 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
     4741 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
     4801 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg
     4861 taccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg
     4921 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4981 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ctagagatct
     5041 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
     5101 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
     5161 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
     5221 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca
     5281 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
     5341 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
     5401 atcacatccg aacataaaca accgttgaca attaatcatc ggcatagtat atcggcatag
     5461 tataatacga caaggtgagg aactaaacca tgggtaccac tcttgacgac acggcttacc
     5521 ggtaccgcac cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca
     5581 ccaccgacac cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc
     5641 cggtggaccc gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg
     5701 acggggagga cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc
     5761 tggcgggctt cgtggtcgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca
     5821 tcgaggtcgc cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga
     5881 cggagttcgc ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg
     5941 caccggcgat ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc
     6001 tgtacgacgg caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgcccct
     6061 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     6121 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     6181 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     6241 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtgtc
     6301 gaaaacgggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6361 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6421 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6481 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6541 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6601 cgcgaatttt aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg
     6661 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
     6721 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     6781 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     6841 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac
     6901 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac
     6961 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc
     7021 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata
     7081 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg
     7141 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac
     7201 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg
     7261 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta
     7321 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat
     7381 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa
     7441 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat
     7501 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc
     7561 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa
     7621 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct
     7681 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaaag
     7741 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa aagctgtggt
     7801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     7861 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     7921 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     7981 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     8041 ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg
     8101 gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag
     8161 aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt
     8221 aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt
     8281 aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt
     8341 ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca
     8401 agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa
     8461 aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt
     8521 aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt
     8581 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8641 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8701 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8761 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8821 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8881 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8941 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9001 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9061 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9121 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9181 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9241 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9301 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9361 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9421 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9481 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9541 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9601 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9661 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9841 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     9901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10381 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10441 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10501 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10561 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc
    10621 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg ttgtgtggaa
    10681 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    10741 caattaaccc tcactaaagg gaacaaaagc tggagctc
//
