LOCUS       pXJ75_pDawn_sfGF        7917 bp ds-DNA     circular     17-MAR-2015
DEFINITION  Expression vector pDawn, complete sequence.
ACCESSION   JN579121
VERSION     JN579121.1  GI:383460062
KEYWORDS    .
SOURCE      Expression vector pDawn
  ORGANISM  Expression vector pDawn other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Ohlendorf,R., Vidavski,R.R., Eldar,A., Moffat,K. and Moglich,A.
  TITLE     From dusk till dawn: one-plasmid systems for light-regulated gene
            expression
  JOURNAL   J. Mol. Biol. 416 (4), 534-542 (2012)
   PUBMED   22245580
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Ohlendorf,R., Moffat,K. and Moeglich,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2011) Biophysikalische Chemie, Humboldt
            Universitaet zu Berlin, Invalidenstrasse 42, Berlin 10115, Germany
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /note="T7 terminator"
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(164..883)
                     /label=superfolder GFP orf
                     /ApEinfo_fwdcolor=#03ff44
                     /ApEinfo_revcolor=#03ff44
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(902..907)
                     /note="multiple cloning site"
                     /label=multiple cloning site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(890..901)
                     /label=RBS (BBa_B0034)
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(1043..1091)
                     /note="lambda pR promoter"
                     /label=lambda pR promoter
                     /ApEinfo_fwdcolor=#80ffff
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1265..2011)
                     /note="lambda cI-LVA"
                     /codon_start=1
                     /transl_table=11
                     /product="cI-LVA"
                     /protein_id="AFH34992.1"
                     /db_xref="GI:383460063"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFGAANDENYALVA"
                     /label=cI-LVA
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2036..2282)
                     /note="FixK2 promoter"
                     /label=FixK2 promoter
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2655..4408
                     /note="YF1/FixJ bicistronic operon"
                     /label=YF1/FixJ bicistronic operon
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2655..3797
                     /codon_start=1
                     /transl_table=11
                     /product="YF1"
                     /protein_id="AFH34994.1"
                     /db_xref="GI:383460065"
                     /translation="MASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQ
                     GFVQMTGYETEEILGKNCRFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMF
                     WNELNIDPMEIEDKTYFVGIQNDITEHQQTQARLQELQSELVHVSRLSAMGEMASALA
                     HELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVA
                     RGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNL
                     FRNALEAMAQSQRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTG
                     MGVGLSISRSIIEAHGGRMWAESNASGGATFRFTLPAADEMIGGLA"
                     /label=YF1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3794..4411
                     /codon_start=1
                     /transl_table=11
                     /product="FixJ"
                     /protein_id="AFH34995.1"
                     /db_xref="GI:383460066"
                     /translation="MTTKGHIYVIDDDAAMRDSLNFLLDSAGFGVTLFDDAQAFLDAL
                     PGLSFGCVVSDVRMPGLDGIELLKRMKAQQSPFPILIMTGHGDVPLAVEAMKLGAVDF
                     LEKPFEDDRLTAMIESAIRQAEPAAKSEAVAQDIAARVASLSPRERQVMEGLIAGLSN
                     KLIAREYDISPRTIEVYRANVMTKMQANSLSELVRLAMRAGMLND"
                     /label=FixJ
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5815..6497)
                     /note="ColE1 origin"
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=#8080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6543..7358
                     /note="KanR resistance marker"
                     /codon_start=1
                     /transl_table=11
                     /product="KanR"
                     /protein_id="AFH34993.1"
                     /db_xref="GI:383460064"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label=KanR
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(7451..7906)
                     /note="M13 origin"
                     /label=M13 origin
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(7583..7889)
                     /note="F1 origin"
                     /label=F1 origin
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtcatcat ttgtacagtt
      181 catccatacc atgcgtgatg cccgctgcgg ttacgaactc cagcagaacc atatgatcgc
      241 gtttctcgtt cggatcttta gacagaacgc tttgcgtgct cagatagtga ttgtctggca
      301 gcagaacagg accatcaccg attggagtgt tttgctggta gtgatcagcc agctgcacgc
      361 tgccatcctc cacgttgtgg cgaattttaa aattcgcttt aatgccattt ttttgtttat
      421 cggcggtgat gtaaacattg tggctgttaa aattgtattc cagcttatgg cccaggatat
      481 tgccgtcttc tttaaagtca atgcctttca gctcaatgcg gtttaccagg gtatcgcctt
      541 caaatttcac ttccgcacgc gttttgtacg tgccgtcatc cttaaaggaa atcgtgcgtt
      601 cctgcacata gccttccggc atggcggact tgaagaagtc atgctgcttc atatggtccg
      661 gataacgagc aaagcactga acaccataag tcagcgtcgt taccagagtc ggccaaggta
      721 ccggcagttt accagtagta cagatgaact tcagcgtcag tttaccatta gttgcgtcac
      781 cttcaccctc gccacgcacg gaaaacttat gaccgttgac atcaccatcc agttccacca
      841 gaatagggac gacaccagtg aacagctctt cgcctttacg catctagtat ttctcctctt
      901 tggatccgac ccatttgctg tccaccagtc atgctagcca tatggctgcc gcgcggcacc
      961 aggccgctgc tgtgatgatg atgatgatgg ctgctgccca tggtatatct ccttcttaaa
     1021 gttaaacaaa attatttcta gagcaaccat tatcaccgcc agaggtaaaa tagtcaacac
     1081 gcacggtgtt actctagtat ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
     1141 ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     1201 tgagcctttc gttttatttg atgcctggct ctagtagcga tctacactag cactatcagc
     1261 gttattaagc tactaaagcg tagttttcgt cgtttgcagc gccaaacgtc tcttcaggcc
     1321 actgactagc gataactttc cccacaacgg aacaactctc attgcatggg atcattgggt
     1381 actgtgggtt tagtggttgt aaaaacacct gaccgctatc cctgatcagt ttcttgaagg
     1441 taaactcatc acccccaagt ctggctatgc agaaatcacc tggctcaaca gcctgctcag
     1501 ggtcaacgag aattaacatt ccgtcaggaa agctcggctt ggagcctgtt ggtgcggtca
     1561 tggaattacc ttcaacctca agccagaatg cagaatcact ggcttttttg gttgtgctta
     1621 cccatctctc cgcatcacct ttggtaaagg ttctaagctc aggtgagaac atccctgcct
     1681 gaacatgaga aaaaacaggg tactcatact cacttctaag tgacggctgc atactaaccg
     1741 cttcatacat ctcgtagatt tctctggcga ttgaagggct aaattcttca acgctaactt
     1801 tgagaatttt tgcaagcaat gcggcgttat aagcatttaa tgcattgatg ccattaaata
     1861 aagcaccaac gcctgactgc cccatcccca tcttgtctgc gacagattcc tgggataagc
     1921 caagttcatt tttctttttt tcataaattg ctttaaggcg acgtgcgtcc tcaagctgct
     1981 cttgtgttaa tggtttcttt tttgtgctca tctagtattt ctcctctttt ctagactccg
     2041 ttgtgatgac gcattggtac gcggtatcgg gaggttcgaa aatttcgagc gatatcttaa
     2101 ggggggtgcc ttacgtagaa ccccgtaggt catgcccgag gccggtcctg gatggcgcgg
     2161 cggatacgct tgagcaggtt ttcgtcgaga agcggcttca aaaccacgtc ttttacgccg
     2221 gcctcggcgg cccgggtcga gatgttttcg tccggatagc cggtgatcag gatcacgggc
     2281 gtagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     2341 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     2401 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     2461 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     2521 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca
     2581 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
     2641 tgagggtggt gaatgtggct agttttcaat catttgggat accaggacag ctggaagtca
     2701 tcaaaaaagc acttgatcac gtgcgagtcg gtgtggtaat tacagatccc gcacttgaag
     2761 ataatcctat tgtctacgta aatcaaggct ttgttcaaat gaccggctac gagaccgagg
     2821 aaattttagg aaagaactgt cgcttcttac aggggaaaca cacagatcct gcagaagtgg
     2881 acaacatcag aaccgcttta caaaataaag aaccggtcac cgttcagatc caaaactaca
     2941 aaaaagacgg aacgatgttc tggaatgaat taaatattga tccaatggaa atagaggata
     3001 aaacgtattt tgtcggtatt cagaatgata tcaccgagca ccagcagacc caggcgcgcc
     3061 tccaggaact gcaatccgag ctcgtccacg tctccaggct gagcgccatg ggcgaaatgg
     3121 cgtccgcgct cgcgcacgag ctcaaccagc cgctggcggc gatcagcaac tacatgaagg
     3181 gctcgcggcg gctgcttgcc ggcagcagtg atccgaacac accgaaggtc gaaagcgccc
     3241 tggaccgcgc cgccgagcag gcgctgcgcg ccggccagat catccggcgc ctgcgcgact
     3301 tcgttgcccg cggcgaatcg gagaagcggg tcgagagtct ctccaagctg atcgaggagg
     3361 ccggcgcgct cgggcttgcc ggcgcgcgcg agcagaacgt gcagctccgc ttcagtctcg
     3421 atccgggcgc cgatctcgtt ctcgccgacc gggtgcagat ccagcaggtc ctggtcaacc
     3481 tgttccgcaa cgcgctggaa gcgatggctc agtcgcagcg acgcgagctc gtcgtcacca
     3541 acacccccgc cgccgacgac atgatcgagg tcgaagtgtc cgacaccggc agcggtttcc
     3601 aggacgacgt cattccgaac ctgtttcaga ctttcttcac caccaaggac accggcatgg
     3661 gcgtgggact gtccatcagc cgctcgatca tcgaagctca cggcgggcgc atgtgggccg
     3721 agagcaacgc atcgggcggg gcgaccttcc gcttcaccct cccggcagcc gacgagatga
     3781 taggaggtct agcatgacga ccaagggaca tatctacgtc atcgacgacg acgcggcgat
     3841 gcgggattcg ctgaatttcc tgctggattc tgccggcttc ggcgtcacgc tgtttgacga
     3901 cgcgcaagcc tttctcgacg ccctgccggg tctctccttc ggctgcgtcg tctccgacgt
     3961 gcgcatgccg ggccttgacg gcatcgagct gttgaagcgg atgaaggcgc agcaaagccc
     4021 ctttccgatc ctcatcatga ccggtcacgg cgacgtgccg ctcgcggtcg aggcgatgaa
     4081 gttaggggcg gtggactttc tggaaaagcc tttcgaggac gaccgcctca ccgccatgat
     4141 cgaatcggcg atccgccagg ccgagccggc cgccaagagc gaggccgtcg cgcaggatat
     4201 cgccgcccgc gtcgcctcgt tgagccccag ggagcgccag gtcatggaag ggctgatcgc
     4261 cggcctttcc aacaagctga tcgcccgcga gtacgacatc agcccgcgca ccatcgaggt
     4321 gtatcgggcc aacgtcatga ccaagatgca ggccaacagc ctttcggagc tggttcgcct
     4381 cgcgatgcgc gccggcatgc tcaacgattg acaattgatg taagttagct cactcattag
     4441 gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     4501 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     4561 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     4621 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     4681 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     4741 gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
     4801 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
     4861 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     4921 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
     4981 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     5041 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     5101 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
     5161 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
     5221 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
     5281 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
     5341 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5401 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5461 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     5521 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     5581 tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5641 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6241 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6481 agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     6541 ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat tccaacatgg
     6601 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     6661 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     6721 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     6781 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     6841 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     6901 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     6961 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     7021 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     7081 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     7141 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     7201 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     7261 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     7321 aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat gagcggatac
     7381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7441 gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7501 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7561 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7621 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7681 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7741 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7801 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7861 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//
