LOCUS       pDawn_Ag43             10440 bp ds-DNA     circular     08-FEB-2018
DEFINITION  Expression vector pDawn, complete sequence.
ACCESSION   JN579121
VERSION     JN579121.1  GI:383460062
KEYWORDS    .
SOURCE      Expression vector pDawn
  ORGANISM  Expression vector pDawn other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Ohlendorf,R., Vidavski,R.R., Eldar,A., Moffat,K. and Moglich,A.
  TITLE     From dusk till dawn: one-plasmid systems for light-regulated gene
            expression
  JOURNAL   J. Mol. Biol. 416 (4), 534-542 (2012)
   PUBMED   22245580
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Ohlendorf,R., Moffat,K. and Moeglich,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2011) Biophysikalische Chemie, Humboldt
            Universitaet zu Berlin, Invalidenstrasse 42, Berlin 10115, Germany
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /note="T7 terminator"
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3305..3310)
                     /note="multiple cloning site"
                     /label=multiple cloning site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(164..3283)
                     /label=BBa_K346007 (Ag43/flu ORF)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             9059..9850
                     /label=specR
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(3446..3494)
                     /note="lambda pR promoter"
                     /label=lambda pR promoter
                     /ApEinfo_fwdcolor=#80ffff
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3668..4414)
                     /note="lambda cI-LVA"
                     /codon_start=1
                     /transl_table=11
                     /product="cI-LVA"
                     /protein_id="AFH34992.1"
                     /db_xref="GI:383460063"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFGAANDENYALVA"
                     /label=cI-LVA
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      9858..9963
                     /label=terminator T0
                     /ApEinfo_fwdcolor=#ff0517
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(4439..4685)
                     /note="FixK2 promoter"
                     /label=FixK2 promoter
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        8911..9058
                     /label=specR promoter from pKD13
                     /ApEinfo_fwdcolor=#a5ce0b
                     /ApEinfo_revcolor=#a5ce0b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5058..6811
                     /note="YF1/FixJ bicistronic operon"
                     /label=YF1/FixJ bicistronic operon
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5058..6200
                     /codon_start=1
                     /transl_table=11
                     /product="YF1"
                     /protein_id="AFH34994.1"
                     /db_xref="GI:383460065"
                     /translation="MASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQ
                     GFVQMTGYETEEILGKNCRFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMF
                     WNELNIDPMEIEDKTYFVGIQNDITEHQQTQARLQELQSELVHVSRLSAMGEMASALA
                     HELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVA
                     RGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNL
                     FRNALEAMAQSQRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTG
                     MGVGLSISRSIIEAHGGRMWAESNASGGATFRFTLPAADEMIGGLA"
                     /label=YF1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6197..6814
                     /codon_start=1
                     /transl_table=11
                     /product="FixJ"
                     /protein_id="AFH34995.1"
                     /db_xref="GI:383460066"
                     /translation="MTTKGHIYVIDDDAAMRDSLNFLLDSAGFGVTLFDDAQAFLDAL
                     PGLSFGCVVSDVRMPGLDGIELLKRMKAQQSPFPILIMTGHGDVPLAVEAMKLGAVDF
                     LEKPFEDDRLTAMIESAIRQAEPAAKSEAVAQDIAARVASLSPRERQVMEGLIAGLSN
                     KLIAREYDISPRTIEVYRANVMTKMQANSLSELVRLAMRAGMLND"
                     /label=FixJ
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3291..3304)
                     /label=midRBS (BBa_B0031)
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8218..8900)
                     /note="ColE1 origin"
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=#8080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(9974..10429)
                     /note="M13 origin"
                     /label=M13 origin
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(10106..10412)
                     /note="F1 origin"
                     /label=F1 origin
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtcagaag gtcacattca
      181 gtgtggcctg accgttatac ccttcagcgc tgctgccgct gacgctgtgg gcataaccgg
      241 cctgaacgcc cagggtgata ttttcccgga cacgggcttc cagtccggct tgcaggtcca
      301 gtgatgtgcc attctgtgag ggagagaacg tcatcccgct gcttgcagtg gaagtcccca
      361 cacgcatatc tccccgggag ctgaaggtgc ggataacaga aggctgtacc caccagttca
      421 ccggtaattc actcacactg tgttttgcac tgtcacgcag gggggcacgg gatgaggtgc
      481 cttcgccaaa ggtcatatcg ttgtggctgc ccagacggaa accggcacgc acatgttgtg
      541 cactgccatg cccgaacttc acataaccgg cgttgtcctt accgtcatcc agggaaagtc
      601 cctgccaggt atattgcagt tgtggctcca gcatcaggtt gtcagtgata ctgaagggca
      661 gaccggtttc cagtgagccc agccagcccc agccccgggc gcggaagtcg ttattgtccg
      721 atgacgcttt catgctgtgg cgggttccct gtgccacaat gtcagcccac aggccggagg
      781 acgtgtgtac cagattcagg tatccgccca ggctgccggc atcatcccgg accgtgccgg
      841 cacgggagcc gtcatcatcc ttaacatcaa cggaagaatg gccagcagca ccatataccc
      901 ccgcggtcac agacataccg gcaacctctg ttctcatcag gtcaccctcc agacggacga
      961 atccatagct gccgctgctt tccggcgtgg ccccacgggc aataccgcca ttgttatcgt
     1021 gaccgagatg accgccctga atgctgagac ggacgctgtt gttttcacca tttacaccgg
     1081 tctgatggct gcgggagcct gccacaatcc ggtcatagtc cattgcctgt gtcagcatgg
     1141 aggcatacag ggggacttct gcacgataag cattttcact gcgcagatac cagctctcat
     1201 cactgtcccg gttgagggag tagttaaagg caccggcttg cagcctgttc ccctggacaa
     1261 aggccccttc ctccgtggtg gcaccgttaa tggcttccac cacctgaata cccttaccgc
     1321 tggtcgccag ccccgacgca ctgttgccgg cgttcaccag gttcaggatg gtttttccgg
     1381 ttgccctgcc gccgtcaatg accagtctgt cagcattgtt ctgtgccata tccgggcgta
     1441 cacgcaggct gatggtgcca ttctgtccgt tcaggttttt cactttcagg gttgccggta
     1501 cgaacttccc tgtgcgggtg gaggtgaaat gaatctgtcc ggcatggctg aggtcatcca
     1561 ccaccgactg caccgtggcg ttatcgggga tattccaggt ggcaccggag gcgagagtga
     1621 cattcgtggg gtcaatggca ccgttcagca cagtgctgcc ggtcagcttc agggctgtac
     1681 cgcgctgagc aatgacatcc gtggtgacgg tactgtcgtt cagcgtcagc gtgccttcat
     1741 tcaggttgac ggctttctgg gtgagtgtgg tgttgctgac agtgagtgtg ccacttcctg
     1801 atttttccac gctgccgtta ccggtgagag agcctccttg caggagtgca tcgccttcac
     1861 ggatggtcag ggtatcgttg ttcaccgtct tcccggaaag ggtaagtatg gcgccgttat
     1921 tcagcgtggt ggtgcccgcc agtgtgccgc ccctggcggt gaacagtccg ccatttaccg
     1981 tggtatccgt ggccgtatca ccggcgttca gcgtgaatga actgcctttt tccagcatca
     2041 gggcatccgc ctgaccgcct ccgatactga atgcctttcc gtcgctccgg gtaccactga
     2101 cagcggcacc ggaatcggcc agcagtacac cgccatttcc catggatacg gtggtggcgg
     2161 tgccaccgtt gcggacatcc agcgttccgc catcatccac gcgggtatta gtggctgtgt
     2221 gtccggtcag cacatccagg cgtccgccat tttccagtac gacattatca gctttaccct
     2281 ccacaacaga gaatgctccc aggcggttta tgccggtaac ggttgcagcc gtactggtaa
     2341 ccagtgcgcc gccctgcttc agggtgacat tcgtggctgt accaccggcg tccacttgca
     2401 gtctgccctt ctgattaacg gtggtattcc cggccacacc cccgtttttg acaatctgcc
     2461 agccgtcact gttcacaaca gtgtcagacg ctgtaccgcc gttgtgcaca tactggtatc
     2521 ccccattcag cgtggtatcc agtgcgtgac catgtacagt ctggtcgccg ccggcataaa
     2581 ccacagtggt atttgccgtt ccttcagctc tcacaatctg gcgaccgttt ttattgatgg
     2641 ttgtgcgtac ggcatccccg cgaacaaact gcccggtatc accgttttct gcatccggtc
     2701 ccccttccgc cccggtatta acaacggtat ccgttgccac tgtaccgggc ttgacgacct
     2761 gccagccctt atcattaatg acggttcctg tggctatcgc cccctcatgc atccactgtt
     2821 cgccaccatt cagcgtggtg ttcacagccc gtccctgaag gctctgtccg cctccggcac
     2881 tgataaccgt gtctgagaca cttccaccgg ggttcactct ctgaagacca ccactggtga
     2941 cagtcgtttt gttggctgtt ccgccatcct gtacccattg cccgccggta ttggcctcgt
     3001 tatccggccc atactccagc ccggtactga tggtcattcc gttggtcgta ccgaagacaa
     3061 tctggttgtc atgatttgcc agtgttccgc cgttcacggt ttctcccggg tgcacaacga
     3121 tgtcagcagc cagcaccggg agtgacgtga ctgcggcaag agacagtgca accgccacac
     3181 cgccacgttt accccgtgcg cgggccagtt cggaggcaac cacgaaagcg cccgtcatgt
     3241 gattccatac cagcctgtag caggtattca gatgtcgttt cattctagta ggtttcctgt
     3301 gtgaggatcc gacccatttg ctgtccacca gtcatgctag ccatatggct gccgcgcggc
     3361 accaggccgc tgctgtgatg atgatgatga tggctgctgc ccatggtata tctccttctt
     3421 aaagttaaac aaaattattt ctagagcaac cattatcacc gccagaggta aaatagtcaa
     3481 cacgcacggt gttactctag tatataaacg cagaaaggcc cacccgaagg tgagccagtg
     3541 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3601 gactgagcct ttcgttttat ttgatgcctg gctctagtag cgatctacac tagcactatc
     3661 agcgttatta agctactaaa gcgtagtttt cgtcgtttgc agcgccaaac gtctcttcag
     3721 gccactgact agcgataact ttccccacaa cggaacaact ctcattgcat gggatcattg
     3781 ggtactgtgg gtttagtggt tgtaaaaaca cctgaccgct atccctgatc agtttcttga
     3841 aggtaaactc atcaccccca agtctggcta tgcagaaatc acctggctca acagcctgct
     3901 cagggtcaac gagaattaac attccgtcag gaaagctcgg cttggagcct gttggtgcgg
     3961 tcatggaatt accttcaacc tcaagccaga atgcagaatc actggctttt ttggttgtgc
     4021 ttacccatct ctccgcatca cctttggtaa aggttctaag ctcaggtgag aacatccctg
     4081 cctgaacatg agaaaaaaca gggtactcat actcacttct aagtgacggc tgcatactaa
     4141 ccgcttcata catctcgtag atttctctgg cgattgaagg gctaaattct tcaacgctaa
     4201 ctttgagaat ttttgcaagc aatgcggcgt tataagcatt taatgcattg atgccattaa
     4261 ataaagcacc aacgcctgac tgccccatcc ccatcttgtc tgcgacagat tcctgggata
     4321 agccaagttc atttttcttt ttttcataaa ttgctttaag gcgacgtgcg tcctcaagct
     4381 gctcttgtgt taatggtttc ttttttgtgc tcatctagta tttctcctct tttctagact
     4441 ccgttgtgat gacgcattgg tacgcggtat cgggaggttc gaaaatttcg agcgatatct
     4501 taaggggggt gccttacgta gaaccccgta ggtcatgccc gaggccggtc ctggatggcg
     4561 cggcggatac gcttgagcag gttttcgtcg agaagcggct tcaaaaccac gtcttttacg
     4621 ccggcctcgg cggcccgggt cgagatgttt tcgtccggat agccggtgat caggatcacg
     4681 ggcgtagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     4741 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     4801 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     4861 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     4921 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
     4981 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     5041 aattgagggt ggtgaatgtg gctagttttc aatcatttgg gataccagga cagctggaag
     5101 tcatcaaaaa agcacttgat cacgtgcgag tcggtgtggt aattacagat cccgcacttg
     5161 aagataatcc tattgtctac gtaaatcaag gctttgttca aatgaccggc tacgagaccg
     5221 aggaaatttt aggaaagaac tgtcgcttct tacaggggaa acacacagat cctgcagaag
     5281 tggacaacat cagaaccgct ttacaaaata aagaaccggt caccgttcag atccaaaact
     5341 acaaaaaaga cggaacgatg ttctggaatg aattaaatat tgatccaatg gaaatagagg
     5401 ataaaacgta ttttgtcggt attcagaatg atatcaccga gcaccagcag acccaggcgc
     5461 gcctccagga actgcaatcc gagctcgtcc acgtctccag gctgagcgcc atgggcgaaa
     5521 tggcgtccgc gctcgcgcac gagctcaacc agccgctggc ggcgatcagc aactacatga
     5581 agggctcgcg gcggctgctt gccggcagca gtgatccgaa cacaccgaag gtcgaaagcg
     5641 ccctggaccg cgccgccgag caggcgctgc gcgccggcca gatcatccgg cgcctgcgcg
     5701 acttcgttgc ccgcggcgaa tcggagaagc gggtcgagag tctctccaag ctgatcgagg
     5761 aggccggcgc gctcgggctt gccggcgcgc gcgagcagaa cgtgcagctc cgcttcagtc
     5821 tcgatccggg cgccgatctc gttctcgccg accgggtgca gatccagcag gtcctggtca
     5881 acctgttccg caacgcgctg gaagcgatgg ctcagtcgca gcgacgcgag ctcgtcgtca
     5941 ccaacacccc cgccgccgac gacatgatcg aggtcgaagt gtccgacacc ggcagcggtt
     6001 tccaggacga cgtcattccg aacctgtttc agactttctt caccaccaag gacaccggca
     6061 tgggcgtggg actgtccatc agccgctcga tcatcgaagc tcacggcggg cgcatgtggg
     6121 ccgagagcaa cgcatcgggc ggggcgacct tccgcttcac cctcccggca gccgacgaga
     6181 tgataggagg tctagcatga cgaccaaggg acatatctac gtcatcgacg acgacgcggc
     6241 gatgcgggat tcgctgaatt tcctgctgga ttctgccggc ttcggcgtca cgctgtttga
     6301 cgacgcgcaa gcctttctcg acgccctgcc gggtctctcc ttcggctgcg tcgtctccga
     6361 cgtgcgcatg ccgggccttg acggcatcga gctgttgaag cggatgaagg cgcagcaaag
     6421 cccctttccg atcctcatca tgaccggtca cggcgacgtg ccgctcgcgg tcgaggcgat
     6481 gaagttaggg gcggtggact ttctggaaaa gcctttcgag gacgaccgcc tcaccgccat
     6541 gatcgaatcg gcgatccgcc aggccgagcc ggccgccaag agcgaggccg tcgcgcagga
     6601 tatcgccgcc cgcgtcgcct cgttgagccc cagggagcgc caggtcatgg aagggctgat
     6661 cgccggcctt tccaacaagc tgatcgcccg cgagtacgac atcagcccgc gcaccatcga
     6721 ggtgtatcgg gccaacgtca tgaccaagat gcaggccaac agcctttcgg agctggttcg
     6781 cctcgcgatg cgcgccggca tgctcaacga ttgacaattg atgtaagtta gctcactcat
     6841 taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     6901 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     6961 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     7021 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     7081 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     7141 gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     7201 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     7261 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     7321 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     7381 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     7441 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     7501 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     7561 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     7621 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     7681 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     7741 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     7801 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7861 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     7921 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     7981 tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8041 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8161 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8641 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8881 ttaagggatt ttggtcatga acaataaaac agcaagcgaa ccggaattgc cagctggggc
     8941 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     9001 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     9061 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     9121 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     9181 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     9241 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     9301 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     9361 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     9421 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     9481 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     9541 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     9601 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     9661 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     9721 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     9781 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     9841 cggcaaataa gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     9901 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     9961 gcagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
    10021 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
    10081 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
    10141 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
    10201 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
    10261 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
    10321 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
    10381 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//
