LOCUS       pBAD_Ag43               7833 bp ds-DNA     circular     08-FEB-2018
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    4495..4515
                     /label=biobrick suffix
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(4878..5828)
                     /label=repA
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5832..5844)
                     /label=RBS for repA
                     /ApEinfo_fwdcolor=#690f96
                     /ApEinfo_revcolor=#690f96
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(6714..7574)
                     /label=AmpR
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1238..4357
                     /label=BBa_K346007 (Ag43/flu ORF)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5849..5868)
                     /label=repA promoter
                     /ApEinfo_fwdcolor=#80ff80
                     /ApEinfo_revcolor=#80ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4653..4670)
                     /label=VR primer site
                     /ApEinfo_fwdcolor=#80ff80
                     /ApEinfo_revcolor=#80ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4516..4589
                     /label=his operon terminator
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7812..7833
                     /label=BioBricks prefix
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7694..7714
                     /label=VF2 primer site
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7575..7653)
                     /label=Promoter/RBS for AmpR
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1043..1114)
                     /label=PC
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..1210
                     /label=I0500
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1219..1230
                     /label=RBS (BBa_B0034)
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1080..1210
                     /label=pBAD
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1..1079)
                     /label=AraC
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      4366..4494
                     /label=terminator B0015
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT
       61 CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC
      121 AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG
      181 GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG
      241 ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT
      301 GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG
      361 TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT
      421 TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA
      481 CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA
      541 TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG
      601 GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC CTGGCGGGAA
      661 CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA CCACCCCCTG
      721 ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT CGATAAAAAA
      781 ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG CATTAAACGA
      841 GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC TCCCGCCATT
      901 CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG TCTTTTACTG
      961 GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT AACAAAGCGG
     1021 GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA GAAAAGTCCA
     1081 CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT CCATAAGATT
     1141 AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT ACCCGTTTTT
     1201 TTGGGCTAGC tactagagaa agaggagaaa tactagaatg aaacgacatc tgaatacctg
     1261 ctacaggctg gtatggaatc acatgacggg cgctttcgtg gttgcctccg aactggcccg
     1321 cgcacggggt aaacgtggcg gtgtggcggt tgcactgtct cttgccgcag tcacgtcact
     1381 cccggtgctg gctgctgaca tcgttgtgca cccgggagaa accgtgaacg gcggaacact
     1441 ggcaaatcat gacaaccaga ttgtcttcgg tacgaccaac ggaatgacca tcagtaccgg
     1501 gctggagtat gggccggata acgaggccaa taccggcggg caatgggtac aggatggcgg
     1561 aacagccaac aaaacgactg tcaccagtgg tggtcttcag agagtgaacc ccggtggaag
     1621 tgtctcagac acggttatca gtgccggagg cggacagagc cttcagggac gggctgtgaa
     1681 caccacgctg aatggtggcg aacagtggat gcatgagggg gcgatagcca caggaaccgt
     1741 cattaatgat aagggctggc aggtcgtcaa gcccggtaca gtggcaacgg ataccgttgt
     1801 taataccggg gcggaagggg gaccggatgc agaaaacggt gataccgggc agtttgttcg
     1861 cggggatgcc gtacgcacaa ccatcaataa aaacggtcgc cagattgtga gagctgaagg
     1921 aacggcaaat accactgtgg tttatgccgg cggcgaccag actgtacatg gtcacgcact
     1981 ggataccacg ctgaatgggg gataccagta tgtgcacaac ggcggtacag cgtctgacac
     2041 tgttgtgaac agtgacggct ggcagattgt caaaaacggg ggtgtggccg ggaataccac
     2101 cgttaatcag aagggcagac tgcaagtgga cgccggtggt acagccacga atgtcaccct
     2161 gaagcagggc ggcgcactgg ttaccagtac ggctgcaacc gttaccggca taaaccgcct
     2221 gggagcattc tctgttgtgg agggtaaagc tgataatgtc gtactggaaa atggcggacg
     2281 cctggatgtg ctgaccggac acacagccac taatacccgc gtggatgatg gcggaacgct
     2341 ggatgtccgc aacggtggca ccgccaccac cgtatccatg ggaaatggcg gtgtactgct
     2401 ggccgattcc ggtgccgctg tcagtggtac ccggagcgac ggaaaggcat tcagtatcgg
     2461 aggcggtcag gcggatgccc tgatgctgga aaaaggcagt tcattcacgc tgaacgccgg
     2521 tgatacggcc acggatacca cggtaaatgg cggactgttc accgccaggg gcggcacact
     2581 ggcgggcacc accacgctga ataacggcgc catacttacc ctttccggga agacggtgaa
     2641 caacgatacc ctgaccatcc gtgaaggcga tgcactcctg caaggaggct ctctcaccgg
     2701 taacggcagc gtggaaaaat caggaagtgg cacactcact gtcagcaaca ccacactcac
     2761 ccagaaagcc gtcaacctga atgaaggcac gctgacgctg aacgacagta ccgtcaccac
     2821 ggatgtcatt gctcagcgcg gtacagccct gaagctgacc ggcagcactg tgctgaacgg
     2881 tgccattgac cccacgaatg tcactctcgc ctccggtgcc acctggaata tccccgataa
     2941 cgccacggtg cagtcggtgg tggatgacct cagccatgcc ggacagattc atttcacctc
     3001 cacccgcaca gggaagttcg taccggcaac cctgaaagtg aaaaacctga acggacagaa
     3061 tggcaccatc agcctgcgtg tacgcccgga tatggcacag aacaatgctg acagactggt
     3121 cattgacggc ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag
     3181 tgcgtcgggg ctggcgacca gcggtaaggg tattcaggtg gtggaagcca ttaacggtgc
     3241 caccacggag gaaggggcct ttgtccaggg gaacaggctg caagccggtg cctttaacta
     3301 ctccctcaac cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc
     3361 agaagtcccc ctgtatgcct ccatgctgac acaggcaatg gactatgacc ggattgtggc
     3421 aggctcccgc agccatcaga ccggtgtaaa tggtgaaaac aacagcgtcc gtctcagcat
     3481 tcagggcggt catctcggtc acgataacaa tggcggtatt gcccgtgggg ccacgccgga
     3541 aagcagcggc agctatggat tcgtccgtct ggagggtgac ctgatgagaa cagaggttgc
     3601 cggtatgtct gtgaccgcgg gggtatatgg tgctgctggc cattcttccg ttgatgttaa
     3661 ggatgatgac ggctcccgtg ccggcacggt ccgggatgat gccggcagcc tgggcggata
     3721 cctgaatctg gtacacacgt cctccggcct gtgggctgac attgtggcac agggaacccg
     3781 ccacagcatg aaagcgtcat cggacaataa cgacttccgc gcccggggct ggggctggct
     3841 gggctcactg gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca
     3901 actgcaatat acctggcagg gactttccct ggatgacggt aaggacaacg ccggttatgt
     3961 gaagttcggg catggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa
     4021 cgatatgacc tttggcgaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca
     4081 cagtgtgagt gaattaccgg tgaactggtg ggtacagcct tctgttatcc gcaccttcag
     4141 ctcccgggga gatatgcgtg tggggacttc cactgcaagc agcgggatga cgttctctcc
     4201 ctcacagaat ggcacatcac tggacctgca agccggactg gaagcccgtg tccgggaaaa
     4261 tatcaccctg ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg
     4321 gtataacggt caggccacac tgaatgtgac cttctgatac tagagccagg catcaaataa
     4381 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     4441 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     4501 tagcggccgc tgcagtccgg caaaaaaacg ggcaaggtgt caccaccctg ccctttttct
     4561 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
     4621 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat ctcgaggtta
     4681 cattgtcgat ctgttcatgg tgaacagctt taaatgcacc aaaaactcgt aaaagctctg
     4741 atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata
     4801 tctaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
     4861 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
     4921 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catgcttact ttgcatgtca
     4981 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
     5041 gtttttctta gtccgttacg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
     5101 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
     5161 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
     5221 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
     5281 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
     5341 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
     5401 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
     5461 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
     5521 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
     5581 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
     5641 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
     5701 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
     5761 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
     5821 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
     5881 agtcaatgat aattacatgt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
     5941 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     6001 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     6061 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     6121 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     6181 aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc
     6241 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
     6301 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
     6361 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
     6421 gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt
     6481 ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg
     6541 cagcggtatc atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa
     6601 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     6661 ttactggatc tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa
     6721 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6781 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6841 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6901 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6961 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7021 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7081 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7141 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7201 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7261 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7321 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7381 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7441 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7501 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7561 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7621 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7681 acatttccca tggtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7741 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
     7801 gatgatttct ggaattcgcg gccgcttcta gag  
//
