LOCUS       Exported                6390 bp ds-DNA     circular SYN 12-FEB-2018
DEFINITION  Ligation of product* into Cox8a-pHluorin-sems*.
ACCESSION   .
VERSION     .
KEYWORDS    pSems-SDHC-pHL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 12, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
COMMENT     Vector molecule:  Cox8a-pHluorin-sems*
               Fragment ends: EcoRI and EcoRV
               Fragment size: 5936
            Insert molecule:  product*
               Fragment ends: EcoRV and EcoRI
               Fragment size: 454
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    774..2111
                     /label=ORF-1
     misc_feature    918..1367
                     /label=SDHC
     misc_feature    1380..2096
                     /label=pHluorin
     misc_feature    3077..3877
                     /label=Neomycin
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatcatg gctgcgctgt tgctgagaca cgttggtcgt cattgcctcc
      961 gagcccactt tagccctcag ctctgtatca gaaatgctgt tcctttggga accacggcca
     1021 aagaagagat ggagcggttc tggaataaga atataggttc aaaccgtcct ctgtctcccc
     1081 acattactat ctacagttgg tctcttccca tggcgatgtc catctgccac cgtggcactg
     1141 gtattgcttt gagtgcagat gtgggaccta ggaaaaggcc tgaagattcc ccagctatac
     1201 cagtctggag tggttgtcct ggttcttact gtgttgtcct ctatggggct ggcagccatg
     1261 tgaagaaagg aggctcccag catcatcttc ctacacatta ttacattcac ccatctttct
     1321 gtttgtcatt cttatctcca gcctgggaaa agttctcctt atttgttgaa ttcaccatga
     1381 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     1441 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     1501 ttacccttaa atttatttgc actactggaa aactacctgt tccttggcca acacttgtca
     1561 ctactttaac ttatggtgtt caatgctttt caagataccc agatcatatg aaacggcatg
     1621 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1681 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1741 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1801 aatacaacta taacgatcac caggtgtaca tcatggcaga caaacaaaag aatggaatca
     1861 aagctaactt caaaattaga cacaacattg aagatggagg cgttcaacta gcagaccatt
     1921 atcaacaaaa tactccaatt ggcgatgggc ccgtcctttt accagacaac cattacctgt
     1981 ttacaacttc tactctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     2041 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa accggtggat
     2101 ccctcgagtg aggcggccgc atagataact gatccagtgt gctggaatta attcgctgtc
     2161 tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga cttctgcgct
     2221 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc
     2281 tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt caagcttgag
     2341 gtgtggcagg cttgagatct ggccatacac ttgagtgaca atgacatcca ctttgccttt
     2401 ctctccacag gtgtccactc ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc
     2461 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2521 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     2581 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     2641 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     2701 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     2761 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     2821 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     2881 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     2941 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     3001 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgggat
     3061 aattcctgca gccaatatgg gatcggccat tgaacaagat ggattgcacg caggttctcc
     3121 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3181 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3241 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     3301 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3361 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3421 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3481 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3541 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3601 caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg
     3661 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3721 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3781 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3841 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat tctctagata
     3901 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     3961 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtcga
     4141 gtgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     4201 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4801 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     4861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5041 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6241 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6301 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6361 acatttcccc gaaaagtgcc acctgacgtc
//
