LOCUS       pENN.AAV.TBG.PI.        5545 bp    DNA     circular     21-OCT-2017
SOURCE      
  ORGANISM  
COMMENT       #1: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20TBG%20PI%20%28p1015%29?sel=1259-1001">pENN AAV TBG PI (p1015)</a> 
                parent position: from 1259 to 1001
                original length: 4213
                molecule position: from 2330 to 1000
                Left Terminus
                    SalI site #1
                Right Terminus
                    PstI site #1
              #2: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20CMV%20PI-Cre%28p1097%29?sel=798-2126">pENN AAV CMV PI-Cre(p1097)</a> 
                parent position: from 798 to 2126
                original length: 1329
                molecule position: from 1001 to 2329
                Left Terminus
                    PstI site #2
                Right Terminus
                    SalI site #1
            
            Map modified after 3bp insertion confirmed at  position 2316.----SS1/16/08
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11992900|
COMMENT     VNTDBDATE|-11992900|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENN.AAV.TBG.PI.Cre.rBG(p1091)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Xiangyang Zhou|
COMMENT     VNTOAUTHORTEL|215-898-7117|
COMMENT     VNTOAUTHOREML|zhou3@mail.med.upenn.edu|
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FEATURES             Location/Qualifiers
     promoter        442..901
                     /vntifkey="29"
                     /label=TBG
     TATA_signal     885..888
                     /vntifkey="41"
                     /label=TATA
     enhancer        221..320
                     /vntifkey="9"
                     /label=Alpha\mic/bik
     enhancer        327..426
                     /vntifkey="9"
                     /label=Alpha\mic/bik
     polyA_signal    2380..2506
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(2595..2724)
                     /vntifkey="34"
                     /label=3'\ITR
     rep_origin      4518..5106
                     /vntifkey="33"
                     /label=Origin\of\replication
     misc_marker     3487..4344
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      complement(2901..3356)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             1273..2304
                     /vntifkey="4"
                     /label=Cre
     intron          1027..1159
                     /vntifkey="15"
                     /label=Intron_1
                     /note="chimeric intron"
BASE COUNT     1369 a      1367 c      1359 g      1450 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gaattcgccc ttaagctagc aggttaattt ttaaaaagca 
      241 gtcaaaagtc caagtggccc ttggcagcat ttactctctc tgtttgctct ggttaataat 
      301 ctcaggagca caaacattcc agatccaggt taatttttaa aaagcagtca aaagtccaag 
      361 tggcccttgg cagcatttac tctctctgtt tgctctggtt aataatctca ggagcacaaa 
      421 cattccagat ccggcgcgcc agggctggaa gctacctttg acatcatttc ctctgcgaat 
      481 gcatgtataa tttctacaga acctattaga aaggatcacc cagcctctgc ttttgtacaa 
      541 ctttccctta aaaaactgcc aattccactg ctgtttggcc caatagtgag aactttttcc 
      601 tgctgcctct tggtgctttt gcctatggcc cctattctgc ctgctgaaga cactcttgcc 
      661 agcatggact taaacccctc cagctctgac aatcctcttt ctcttttgtt ttacatgaag 
      721 ggtctggcag ccaaagcaat cactcaaagt tcaaacctta tcattttttg ctttgttcct 
      781 cttggccttg gttttgtaca tcagctttga aaataccatc ccagggttaa tgctggggtt 
      841 aatttataac taagagtgct ctagttttgc aatacaggac atgctataaa aatggaaaga 
      901 tgttgctttc tgagagacag ctttattgcg gtagtttatc acagttaaat tgctaacgca 
      961 gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc gtgaggcact 
     1021 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg 
     1081 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac 
     1141 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt 
     1201 acttaatacg actcactata ggctagcctc gagaattgta cactttactt aaaaccatta 
     1261 tctgagtgtg aaatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc 
     1321 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg 
     1381 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc 
     1441 aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta 
     1501 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac 
     1561 atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt 
     1621 atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg 
     1681 ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag 
     1741 gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa 
     1801 attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat 
     1861 attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg 
     1921 gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac 
     1981 tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta 
     2041 tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag 
     2101 gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg 
     2161 cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc 
     2221 aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg 
     2281 cgcctgctgg aagatggcga ttagccatta acgcggcgtg gtacctctag agtcgacccg 
     2341 ggcggcctcg aggacggggt gaactacgcc tgaggatccg atctttttcc ctctgccaaa 
     2401 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 
     2461 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag caattcgttg 
     2521 atctgaattt cgaccaccca taatacccat taccctggta gataagtagc atggcgggtt 
     2581 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 
     2641 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 
     2701 ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca 
     2761 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 
     2821 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 
     2881 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2941 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     3001 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     3061 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     3121 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     3181 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     3241 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     3301 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 
     3361 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     3421 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     3481 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 
     3541 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 
     3601 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 
     3661 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 
     3721 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 
     3781 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 
     3841 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 
     3901 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 
     3961 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 
     4021 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 
     4081 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 
     4141 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 
     4201 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 
     4261 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
     4321 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
     4381 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
     4441 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 
     4501 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 
     4561 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 
     4621 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 
     4681 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 
     4741 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 
     4801 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 
     4861 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 
     4921 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 
     4981 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 
     5041 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 
     5101 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 
     5161 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 
     5221 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 
     5281 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 
     5341 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 
     5401 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 
     5461 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 
     5521 agatttaatt aaggccttaa ttagg 
//
