LOCUS       Exported                5680 bp ds-DNA     circular SYN 15-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 15, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
COMMENT     LOCUS pCSDest 5808 bp DNA SYN DEFINITION pCSDest ACCESSION KEYWORDS 
            SOURCE ORGANISM other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="synthetic DNA construct"
                     /label=pCSDest
     primer_bind     34..52
                     /label=Set 1 (9)
     misc_feature    151..160
                     /label=Kozak
                     /note="Kozak"
     misc_feature    166..1659
                     /label=TDP43
                     /note="TDP43"
     misc_feature    178..396
                     /label=Ub-like domain
                     /note="Ub-like domain (Mompean et al., 2016)"
     misc_feature    478..1185
                     /label=shsfGFP2
                     /note="shsfGFP2"
     misc_feature    1201..1659
                     /label=Low-complexity region
                     /note="Low-complexity region"
     misc_feature    1297..1299
                     /label=295
                     /note="295"
     misc_feature    1339..1341
                     /label=309
                     /note="309"
     misc_feature    1372..1458
                     /label=Highly conserved region
                     /note="Highly conserved region"
     misc_feature    1423..1425
                     /label=Variable part to introduce mutation
                     /note="Variable part to introduce mutation"
     misc_feature    1447..1449
                     /label=345
                     /note="345"
     misc_feature    1456..1458
                     /label=348
                     /note="348"
     misc_feature    1516..1518
                     /label=368
                     /note="368"
     misc_feature    1558..1560
                     /label=382
                     /note="382"
     misc_feature    1567..1569
                     /label=385
                     /note="385"
     primer_bind     complement(1707..1724)
                     /label=Set 1 (11)
     misc_feature    1729..1924
                     /label=SV40 late polyA site
                     /note="SV40 late polyA site"
     CDS             complement(1739..1858)
                     /codon_start=1
                     /label=SV40_PA_terminator
                     /translation="VYCSL*WLQIKQ*HHKFHK*SIFFTAF*LWFVQTHQCILS"
     CDS             complement(1753..1772)
                     /codon_start=1
                     /label=EBV_rev_primer
                     /translation="VVCPNS"
     primer_bind     1762..1783
                     /label=Set 1 (2)
     CDS             complement(1975..1994)
                     /codon_start=1
                     /label=T3_promoter
                     /translation="N*PSLK"
     primer_bind     complement(1975..1994)
                     /label=Set 1 (8)
     CDS             complement(2011..2029)
                     /codon_start=1
                     /label=M13_reverse_primer
                     /translation="GNSYDH"
     primer_bind     complement(2011..2029)
                     /label=Set 1 (10)
     CDS             complement(2028..2050)
                     /codon_start=1
                     /label=M13_pUC_rev_primer
                     /translation="SG*QFHT"
     CDS             complement(2064..2093)
                     /codon_start=1
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     primer_bind     complement(2558..2579)
                     /label=Set 1 (7)
     primer_bind     2558..2579
                     /label=Set 1 (6)
     primer_bind     2734..2746
                     /label=Set 1 (5)
     CDS             complement(3176..4036)
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3176..4036)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(4078..4106)
                     /codon_start=1
                     /label=AmpR_promoter
                     /translation="FKYVSAHET"
     misc_feature    complement(4299..4605)
                     /label=f1 origin
                     /note="f1 origin"
     misc_feature    4694..5680
                     /label=simian CMV IE94 enhancer/promoter
                     /note="simian CMV IE94 enhancer/promoter"
     CDS             5598..5639
                     /codon_start=1
                     /label=CMV_fwd_primer
                     /translation="RKWAFH*RKWAVGV"
     primer_bind     5622..5639
                     /label=Set 1 (12)
     primer_bind     5622..5639
                     /label=Set 1 (4)
     primer_bind     5622..5639
                     /label=Set 1 (3)
     primer_bind     5622..5639
                     /label=Set 1
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggatcaaca agtttgtaca
      121 aaaaagcagg ctggcgccgg taccaccggt gccaccatgg gatccatgtc tgaatatatt
      181 cgggtaaccg aagatgagaa cgatgagccc attgaaatac catcggaaga cgatgggacg
      241 gtgctgctct ccacggttac agcccagttt ccaggggcgt gtgggcttcg ctacaggaat
      301 ccagtgtctc agtgtatgag aggtgtccgg ctggtagaag gaattctgca tgccccagat
      361 gctggctggg gaaatctggt gtatgttgtc aactatccaa aagataacaa aagaaaaatg
      421 gatgagacag atgcttcatc agcagtgaaa gtgaaaagag cagtccagaa aacatccagt
      481 aaaggagagg aactatttac aggtactgtg cctatcaaag tcgagctgga cggcgacgta
      541 aacggccaca agttcagcgt gcgtggcgag ggcgagggcg atgccaccga gggcaagctg
      601 accctgaagt tcatctgcac caccggcaag ctgccggtgc cgtggccgac cctcgtgacc
      661 accctgacct acggcgtgca gtgcttcagc cgctacccgg accacatgaa gcgtcacgac
      721 ttcttcaagt ccgccatgcc ggaaggctac gtccaggagc gcaccatcga gttcaaggac
      781 gacggcacct acaagacccg cgcggaggtg aagttcgagg gcgacaccct ggtgaaccgc
      841 atcgagctga agggcaacga cttcaaggag gacggcaaca tcctgggtca caagctggag
      901 tacaaccaca acagccacaa cgtccgtatc gaagccgaca agcagaagaa cggcatcaag
      961 gctaacttca agatccgcca caacgttgag gacggcagcc agcaggaagc cgaccacaaa
     1021 cagcagaaca ccccgatcgg cgacggcccg gtgcgcctgc cggacaacca ctatctgagc
     1081 acccagacca cgctgagcaa agacccgaac gagaagcgcg atcacatggt cctgaaggag
     1141 ttcgtgacgg cagccggtat cactaaaggt gaagatgaaa gtggatccaa tgccgaacct
     1201 aagcacaata gcaatagaca gttagaaaga agtggaagat ttggtggtaa tccaggtggc
     1261 tttgggaatc agggtggatt tggtaatagc agagggggtg gagctggttt gggaaacaat
     1321 caaggtagta atatgggtgg tgggatgaac tttggtgcgt tcagcattaa tccagccatg
     1381 atggctgccg cccaggcagc actacagagc agttggggta tgatgggcat gttagccagc
     1441 cagcagaacc agtcatgccc atcgggtaat aaccaaaacc aaggcaacat gcagagggag
     1501 ccaaaccagg ccttcggttc tggaaataac tcttatagtg gctctaattc tggtgcagca
     1561 attggttggg gatcagcatc caatgcaggg tcgggcagtg gttttaatgg aggctttggc
     1621 tcaagcatgg attctaagtc ttctggctgg ggaatgtaga cccagctttc ttgtacaaag
     1681 tggttgatcc tctcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg
     1741 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1801 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1861 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1921 ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt
     1981 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2041 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2101 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2161 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2221 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2821 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4141 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     4201 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4261 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4321 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4381 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4441 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4501 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4561 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4621 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4681 gctattacgc cagtcgacca tagccaattc aatatggcgt atatggactc atgccaattc
     4741 aatatggtgg atctggacct gtgccaattc aatatggcgt atatggactc gtgccaattc
     4801 aatatggtgg atctggaccc cagccaattc aatatggcgg acttggcacc atgccaattc
     4861 aatatggcgg acttggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt
     4921 ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata
     4981 atggcggcca tattggctat atgccaggat caatatatag gcaatatcca atatggccct
     5041 atgccaatat ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat
     5101 tccatatatg ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac
     5161 catatatggg gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct
     5221 atatatggtc tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc
     5281 cccattgacg tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag
     5341 gaccacccac cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc
     5401 cccctattga cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat
     5461 agtaacttgg caagtacatt actattggaa ggacgccagg gtacattggc agtactccca
     5521 ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca
     5581 atggggaggg gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc
     5641 ctaatgggag gtctatataa gcaatgctcg tttagggaac
//
