LOCUS       p15aC-1D        2937 bp    DNA     circular     12-FEB-2018
FEATURES             Location/Qualifiers
     misc_feature    2278..2937
                     /note="Chloramphenicol marker"
     misc_feature    1130..1273
                     /note="Promoter D"
     misc_feature    1192..1197
                     /note="-35"
     misc_feature    1215..1220
                     /note="-10"
     misc_feature    1227..1227
                     /note="Start site"
     misc_feature    1274..1307
                     /note="RBS"
     misc_feature    1308..2012
                     /note="mycPIS"
     misc_feature    1311..1361
                     /note="myc tag"
     misc_feature    2019..2063
                     /note="Terminator"
     misc_feature    266..1119
                     /note="p15a Origin "
     source          1..2937
                     /dnas_title="p15aC-1D"
ORIGIN      
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG
      181 TATCACGAGG CCCTTTCGTC ACACAGCTAA CACCACGTCG TCCCTATCTG CTGCCCTAGG
      241 ttcagcactc ttctcttTAG ATCTaGCTTC ATGTGGCAGG AGAAAAAAGG CTGCACCGGT
      301 GCGTCAGCAG AATATGTGAT ACAGGATATA TTCCGCTTCC TCGCTCACTG ACTCGCTACG
      361 CTCGGTCGTT CGACTGCGGC GAGCGGAAAT GGCTTACGAA CGGGGCGGAG ATTTCCTGGA
      421 AGATGCCAGG AAGATACTTA ACAGGGAAGT GAGAGGGCCG CGGCAAAGCC GTTTTTCCAT
      481 AGGCTCCGCC CCCCTGACAA GCATCACGAA ATCTGACGCT CAAATCAGTG GTGGCGAAAC
      541 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGCG GCTCCCTCGT GCGCTCTCCT
      601 GTTCCTGCCT TTCGGTTTAC CGGTGTCATT CCGCTGTTAT GGCCGCGTTT GTCTCATTCC
      661 ACGCCTGACA CTCAGTTCCG GGTAGGCAGT TCGCTCCAAG CTGGACTGTA TGCACGAACC
      721 CCCCGTTCAG TCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGA
      781 AAGACATGCA AAAGCACCAC TGGCAGCAGC CACTGGTAAT TGATTTAGAG GAGTTAGTCT
      841 TGAAGTCATG CGCCGGTTAA GGCTAAACTG AAAGGACAAG TTTTGGTGAC TGCGCTCCTC
      901 CAAGCCAGTT ACCTCGGTTC AAAGAGTTGG TAGCTCAGAG AACCTTCGAA AAACCGCCCT
      961 GCAAGGCGGT TTTTTCGTTT TCAGAGCAAG AGATTACGCG CAGACCAAAA CGATCTCAAG
     1021 AAGATCATCT TATTAATCAG ATAAAATATT TCTAGATTTC AGTGCAATTT ATCTCTTCAA
     1081 ATGTAGCACC TGAAGTCAGC CCCATACGAT ATAAGTTGTa GCGGCCGCaC ACAGCTAACA
     1141 CCACGTCGTC CCTATCTGCT GCCCTAGGTC TATGAGTGGT TGCTGGATAA CTTTACGGGC
     1201 ATGCATAAGG CTCGTATAAT ATATTCAGGG AGACCACAAC GGTTTCCCTC TACAAATAAT
     1261 TTTGTTTAAC TTTGAGGGAC TACAAAAGTA CAAGTAAGGA GGTATACATG GAGCAGAAAT
     1321 TGATTAGCGA GGAAGATTTG GGTAGCGACG GTAGCGCCGG TCCGAAAGCT AAAACTACCA
     1381 CACCATTGCA GGTATGTTTG TTTTACCCTA ACCTCATTGG CTACACACGC GTTATTCTTT
     1441 CATTGATTTC CTTCTGCTTG CTCGGCCACT TTCCCATTGC ATTTTTATTT TGTTACATCA
     1501 TTGGTTTTGT GCTGGACGCG GTGGATGGCA TGGTAGCGCG ACGGTTTGGC CAATGCACAC
     1561 AGTTTGGTGC AATACTTGAT ATGTTGACGG ATCGGGCGTC AACGGCTGGT TTGATTGTTG
     1621 TGGTTGTGCA GGTATTACAA CCGCTTCCAC ATTGGGGAGC CACTTCGCTT GCATGTCTTG
     1681 TTTTCCTGGA TATTTCTTCG CATTTCTGCG TCATGTACGT GTCGCTTTAT GCTGGCCGTA
     1741 CTAGTCATAA GGACGTGAGC AGCAGTATTT TTTCACTTCT GCGACTTTAT TATACAAATC
     1801 GTCCGTTTAT GTGTGCACTT TGCGTCGGAC AGGAACTTTT CTACTTAAAC TTATATATGT
     1861 ACGGCGTGTA TGGGATCGCT GAACCTGCGT TTCTGTTCTT TATGGCTTTG ACAGCCGCGT
     1921 TGAGTGCTTT CAAGCAGGTG GTGAATGTGC AACAGCTGCT GGATAGCATG TATCATCTGG
     1981 CGGTTCTGGA TGCTGCGGGA AGCCGCCAGT AATGATGAGT TAATAACAGG CCTGCTGGTA
     2041 ATCGCAGGCC TTTTTATTTT TTTTTTTaGC TAGCaGCAGA TTACGCGCAG AAAAAAAGGA
     2101 TCTCAAGAAG ATCCTTTGAT CTTATTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     2161 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     2221 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     2281 CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG
     2341 GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC
     2401 TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC
     2461 GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC
     2521 AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA
     2581 TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC
     2641 AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC
     2701 GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT
     2761 AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC
     2821 CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC
     2881 TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCAT
//

