LOCUS       p15aC-4D1D             5575 bp    DNA     circular     12-FEB-2018
FEATURES             Location/Qualifiers
     misc_feature    2904..3757
                     /note="p15a Origin "
     misc_feature    2848..2892
                     /note="Terminator"
     misc_feature    379..2835
                     /note="mycPI4K"
     misc_feature    346..378
                     /note="RBS"
     misc_feature    382..432
                     /note="myc tag"
     misc_feature    3768..3911
                     /note="Promoter D"
     misc_feature    3830..3835
                     /note="-35"
     misc_feature    3853..3858
                     /note="-10"
     misc_feature    3865..3865
                     /note="Start site"
     misc_feature    3912..3945
                     /note="RBS"
     misc_feature    3946..4650
                     /note="mycPIS"
     misc_feature    3949..3999
                     /note="myc tag"
     misc_feature    4657..4701
                     /note="Terminator"
     misc_feature    202..345
                     /note="Promoter D"
     misc_feature    264..269
                     /note="-35"
     misc_feature    287..292
                     /note="-10"
     misc_feature    299..299
                     /note="Start site"
     misc_feature    4916..5575
                     /note="Chloramphenicol marker"
     source          1..5575
                     /dnas_title="p15aC-4D1D"
ORIGIN      
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG
      181 TATCACGAGG CCCTTTCGTC ACACAGCTAA CACCACGTCG TCCCTATCTG CTGCCCTAGG
      241 TCTATGAGTG GTTGCTGGAT AACTTTACGG GCATGCATAA GGCTCGTATA ATATATTCAG
      301 GGAGACCACA ACGGTTTCCC TCTACAAATA ATTTTGTTTA ACTTTACAAC ACGAACGAAA
      361 AACATAAGGA GGTACATAAT GGAACAGAAA TTGATTAGCG AAGAGGATTT GGGTAGCGAT
      421 GGCAGCGCAG GCGGAGACAC GATCGTTGAA CCGGCCCCCC TGAAGCCAAC TTCTGAGCCC
      481 GCTCCCGGCC CACCAGGGAA TAATGGtGGC TCCTTGCTAA GTGTCATCAC GGAGGGGGTC
      541 GGGGAGCTCT CGGTGATTGA CCCTGAGGTG GCCCAGAAGG CCTGCCAGGA GGTGCTGGAG
      601 AAAGTCAAGC TCTTGCATGG AGGCGTGGCC ATCTCTAGCA GAGGCACCCC CCTGGAGCTG
      661 GTCAATGGaG AcGGaGTaGA CAGCGAGATC CGTTGCCTGG ACGACCCACC CGCCCAGATA
      721 AGGGAGGAGG AAGATGAGAT GGGGGCCACG GTGGCCTCAG GCACAGCCAA GGGAGCAAGA
      781 AGGCGGCGGC AGAACAACTC GGCCAAACAG TCTTGGCTGC TGAGGCTGTT TGAGTCGAAA
      841 CTGTTTGACA TCTCCATGGC CATTTCATAC CTGTATAACT CCAAGGAGCC TGGAGTGCAG
      901 GCCTACATTG GCAACCGGCT CTTCTGCTTT CGTAATGAGG ATGTGGACTT CTATCTGCCC
      961 CAGTTGCTTA ACATGTACAT CCACATGGAC GAGGACGTGG GTGATGCCAT CAAGCCCTAC
     1021 ATAGTCCACC GCTGCCGCCA GAGCATTAAC TTTTCCCTCC AGTGTGCTCT GTTGCTGGGG
     1081 GCCTACTCTT CAGACATGCA CATTTCCACT CAGCGACACT CCCGTGGGAC CAAGCTACGG
     1141 AAGCTGATCC TCTCAGATGA GCTGAAGCCC GCTCACCGAA AGAGGGAGCT GCCCTCCTTG
     1201 AGCCCAGCCC CCGACACAGG GCTGTCTCCC TCTAAAAGGA CCCACCAGCG CTCTAAGTCA
     1261 GATGCCACCG CCAGCATAAG TCTCAGCAGC AACTTGAAAC GAACAGCCAG CAACCCTAAA
     1321 GTGGAGAATG AGGACGAGCC CGTCAGACTG GCTCCTGAGC GAGAATTCAT CAAGTCCCTG
     1381 ATGGCGATTG GCAAGCGGCT GGCCACACTC CCCACCAAGG AGCAGAAGAC ACAGCGGCTG
     1441 ATCTCAGAGC TCTCCCTGCT CAACCACAAG CTCCCTGCCC GAGTCTGGCT GCCCACTGCT
     1501 GGCTTCGACC ACCACGTGGT CCGCGTGCCC CACACCCAGG CTGTCGTCCT CAATTCTAAG
     1561 GACAAGGCTC CCTACCTGAT CTATGTGGAG GTCCTCGAAT GTGAAAACTT CGACACCACT
     1621 AGTGTCCCCG CCCGAATCCC CGAGAACCGA ATTCGGAGCA CCCGGTCTGT AGAGAACCTG
     1681 CCCGAATGCG GCATCACCCA CGAGCAGCGG GCAGGCAGCT TCAGCACCGT GCCCAACTAT
     1741 GACAACGATG ACGAGGCCTG GTCGGTGGAT GACATAGGCG AGCTGCAGGT GGAGCTCCCT
     1801 GAAGTGCACA CCAACAGCTG TGACAACATC TCCCAGTTCT CCGTGGACAG CATCACCAGC
     1861 CAGGAAAGCA AGGAGCCTGT GTTCATCGCA GCGGGTGACA TCCGACGGCG CCTTTCAGAG
     1921 CAGCTGGCTC ACACCCCCAC AGCCTTCAAA CGAGACCCGG AAGACCCTTC TGCAGTTGCT
     1981 CTCAAAGAGC CCTGGCAGGA GAAAGTACGG CGCATTAGAG AGGGTTCCCC CTATGGCCAT
     2041 CTCCCCAATT GGCGGCTCCT GTCAGTCATC GTCAAGTGTG GGGATGACCT TCGGCAGGAG
     2101 CTGTTGGCCT TTCAGGTGTT AAAGCAACTG CAGTCCATTT GGGAACAGGA GCGAGTACCC
     2161 CTGTGGATCA AGCCATATAA GATTCTTGTG ATTTCGGCCG ACAGTGGCAT GATTGAACCA
     2221 GTGGTCAACG CTGTATCCAT TCACCAGGTG AAGAAGCAGT CACAGCTCTC CCTGCTCGAT
     2281 TACTTCCTAC AGGAGCATGG CAGTTATACC ACTGAGGCAT TCCTCAGCGC CCAGCGCAAT
     2341 TTTGTGCAGA GTTGTGCTGG CTACTGCTTG GGCTGCTACC TGCTGCAGGT CAAGGACAGA
     2401 CACAATGGGA ACATCCTGTT GGACGCAGAA GGCCACATCA TCCACATCGA CTTTGGCTTC
     2461 ATCCTATCCA GCTCACCCCG AAACCTGGGC TTTGAGACAT CAGCCTTCAA ACTGACCACA
     2521 GAGTTTGTtG ACGTGATGGG CGGCCTGGAT GGCGACATGT TCAACTATTA CAAGATGTTG
     2581 ATGCTGCAGG GGCTGATCGC TGCTCGGAAA CACATGGATA AGGTGGTGCA GATCGTAGAG
     2641 ATCATGCAAC AAGGTTCTCA GCTTCCTTGC TTCCATGGCT CCAGCACCAT CCGCAACCTC
     2701 AAGGAGAGGT TCCACATGAG CATGACCGAG GAGCAGCTGC AGCTGCTGGT GGAGCAGATG
     2761 GTGGATGGCA GCATGCGGTC TATAACCACC AAACTCTATG ACGGCTTCCA GTACCTCACC
     2821 AACGGCATCA TGTAAGGATC CTGATGAGTT AATAACAGGC CTGCTGGTAA TCGCAGGCCT
     2881 TTTTATTTTT TTTTTTAGAT CTaGCTTCAT GTGGCAGGAG AAAAAAGGCT GCACCGGTGC
     2941 GTCAGCAGAA TATGTGATAC AGGATATATT CCGCTTCCTC GCTCACTGAC TCGCTACGCT
     3001 CGGTCGTTCG ACTGCGGCGA GCGGAAATGG CTTACGAACG GGGCGGAGAT TTCCTGGAAG
     3061 ATGCCAGGAA GATACTTAAC AGGGAAGTGA GAGGGCCGCG GCAAAGCCGT TTTTCCATAG
     3121 GCTCCGCCCC CCTGACAAGC ATCACGAAAT CTGACGCTCA AATCAGTGGT GGCGAAACCC
     3181 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGCGGC TCCCTCGTGC GCTCTCCTGT
     3241 TCCTGCCTTT CGGTTTACCG GTGTCATTCC GCTGTTATGG CCGCGTTTGT CTCATTCCAC
     3301 GCCTGACACT CAGTTCCGGG TAGGCAGTTC GCTCCAAGCT GGACTGTATG CACGAACCCC
     3361 CCGTTCAGTC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGAAA
     3421 GACATGCAAA AGCACCACTG GCAGCAGCCA CTGGTAATTG ATTTAGAGGA GTTAGTCTTG
     3481 AAGTCATGCG CCGGTTAAGG CTAAACTGAA AGGACAAGTT TTGGTGACTG CGCTCCTCCA
     3541 AGCCAGTTAC CTCGGTTCAA AGAGTTGGTA GCTCAGAGAA CCTTCGAAAA ACCGCCCTGC
     3601 AAGGCGGTTT TTTCGTTTTC AGAGCAAGAG ATTACGCGCA GACCAAAACG ATCTCAAGAA
     3661 GATCATCTTA TTAATCAGAT AAAATATTTC TAGATTTCAG TGCAATTTAT CTCTTCAAAT
     3721 GTAGCACCTG AAGTCAGCCC CATACGATAT AAGTTGTaGC GGCCGCaCAC AGCTAACACC
     3781 ACGTCGTCCC TATCTGCTGC CCTAGGTCTA TGAGTGGTTG CTGGATAACT TTACGGGCAT
     3841 GCATAAGGCT CGTATAATAT ATTCAGGGAG ACCACAACGG TTTCCCTCTA CAAATAATTT
     3901 TGTTTAACTT TGAGGGACTA CAAAAGTACA AGTAAGGAGG TATACATGGA GCAGAAATTG
     3961 ATTAGCGAGG AAGATTTGGG TAGCGACGGT AGCGCCGGTC CGAAAGCTAA AACTACCACA
     4021 CCATTGCAGG TATGTTTGTT TTACCCTAAC CTCATTGGCT ACACACGCGT TATTCTTTCA
     4081 TTGATTTCCT TCTGCTTGCT CGGCCACTTT CCCATTGCAT TTTTATTTTG TTACATCATT
     4141 GGTTTTGTGC TGGACGCGGT GGATGGCATG GTAGCGCGAC GGTTTGGCCA ATGCACACAG
     4201 TTTGGTGCAA TACTTGATAT GTTGACGGAT CGGGCGTCAA CGGCTGGTTT GATTGTTGTG
     4261 GTTGTGCAGG TATTACAACC GCTTCCACAT TGGGGAGCCA CTTCGCTTGC ATGTCTTGTT
     4321 TTCCTGGATA TTTCTTCGCA TTTCTGCGTC ATGTACGTGT CGCTTTATGC TGGCCGTACT
     4381 AGTCATAAGG ACGTGAGCAG CAGTATTTTT TCACTTCTGC GACTTTATTA TACAAATCGT
     4441 CCGTTTATGT GTGCACTTTG CGTCGGACAG GAACTTTTCT ACTTAAACTT ATATATGTAC
     4501 GGCGTGTATG GGATCGCTGA ACCTGCGTTT CTGTTCTTTA TGGCTTTGAC AGCCGCGTTG
     4561 AGTGCTTTCA AGCAGGTGGT GAATGTGCAA CAGCTGCTGG ATAGCATGTA TCATCTGGCG
     4621 GTTCTGGATG CTGCGGGAAG CCGCCAGTAA TGATGAGTTA ATAACAGGCC TGCTGGTAAT
     4681 CGCAGGCCTT TTTATTTTTT TTTTTaGCTA GCaGCAGATT ACGCGCAGAA AAAAAGGATC
     4741 TCAAGAAGAT CCTTTGATCT TATTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
     4801 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
     4861 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACG
     4921 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA
     4981 AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT
     5041 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT
     5101 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA
     5161 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA
     5221 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG
     5281 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT
     5341 CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA
     5401 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA
     5461 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT
     5521 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCAT
//

