LOCUS       Exported                8242 bp ds-DNA     circular SYN 14-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1z-55o2kyd
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2018 from SnapGene 4.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..951)
                     /label=RepA
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     primer_bind     300..315
                     /label=WT071 seq RepA 300 FP
     primer_bind     928..951
                     /label=WT072 seq
     rep_origin      complement(955..1700)
                     /direction=LEFT
                     /label=pSC101
                     /note="pSC101"
     primer_bind     1682..1700
                     /label=WT014 seq
     modified_base   1701..1715
                     /label=USER junction
                     /note="USER junction"
     terminator      1716..1760
                     /note="rrnB1 transcriptional terminator"
     primer_bind     complement(1739..1776)
                     /label=WT079
     misc_RNA        1753..1760
                     /label=gRNA binding site, discarded
                     /note="gRNA binding site"
                     /note="/modified_by=User"
     primer_bind     1761..1797
                     /label=WT005
     modified_base   1761..1776
                     /label=USER Linker
                     /note="USER Linker"
     protein_bind    1796..1814
                     /note="tetR binding site"
     promoter        1815..1850
                     /label=tet-regulated promoter
                     /note="anhydrotetracycline induction"
                     /note="/dnas_title=tet-regulated promoter"
                     /note="/vntifkey=30"
     primer_bind     1835..1852
                     /label=WT203 seq
     protein_bind    1850..1868
                     /note="tetR binding site"
     primer_bind     complement(1898..1915)
                     /label=WT032 tet ColEI aadA RP from pWT002
     primer_bind     1901..1919
                     /label=WT031
     modified_base   1901..1915
                     /label=USER junction
                     /note="USER junction"
     RBS             1916..1930
                     /label=SD8
                     /note="SD8"
                     /note="Ringquist and Gold"
     CDS             1931..2617
                     /codon_start=1
                     /label=rAPOBEC1
                     /note="/ApEinfo_fwdcolor=#f8d3a9"
                     /note="/ApEinfo_revcolor=#f8d3a9"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     misc_feature    1931..1933
                     /label=Initiation 8k
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
     misc_feature    2111..2119
                     /label=conserved residues
                     /note="/ApEinfo_fwdcolor=#f8d3a9"
                     /note="/ApEinfo_revcolor=#f8d3a9"
     misc_feature    2111..2113
                     /label=H61 essential, Zn coordination
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
     misc_feature    2204..2209
                     /label=conserved
                     /note="/ApEinfo_fwdcolor=#f8d3a9"
                     /note="/ApEinfo_revcolor=#f8d3a9"
     misc_feature    2207..2209
                     /label=C93 Zn coordination
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
     misc_feature    2216..2218
                     /label=C96 Zn coordination
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
     CDS             2618..2665
                     /codon_start=1
                     /label=XTEN linker
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
                     /translation="SGSETPGTSESATPES"
     CDS             2666..6766
                     /codon_start=1
                     /label=dCas9
                     /note="/ApEinfo_fwdcolor=#b7e6d7"
                     /note="/ApEinfo_revcolor=#b7e6d7"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     2703..2727
                     /label=ACK174
                     /note="/ApEinfo_fwdcolor=#faac61"
                     /note="/ApEinfo_revcolor=#faac61"
     primer_bind     complement(2901..2922)
                     /label=bCas9-1
     misc_feature    6742..6766
                     /label=Gibson homology
                     /note="/ApEinfo_fwdcolor=#d59687"
                     /note="/ApEinfo_revcolor=#d59687"
     misc_feature    6779..7033
                     /label=from Alexis' gblock
                     /note="/ApEinfo_fwdcolor=#85dae9"
                     /note="/ApEinfo_revcolor=#85dae9"
     misc_feature    6779..7030
                     /label=ugi
                     /note="/ApEinfo_fwdcolor=#9eafd2"
                     /note="/ApEinfo_revcolor=#9eafd2"
     misc_feature    6779..6809
                     /label=priming
                     /note="/ApEinfo_fwdcolor=#c7b0e3"
                     /note="/ApEinfo_revcolor=#c7b0e3"
     misc_feature    complement(6995..7030)
                     /label=priming
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
     primer_bind     complement(7007..7046)
                     /label=WT206
     primer_bind     7034..7050
                     /label=WT034 RP sgRNA promoter from pWT003a
     misc_feature    7034..7046
                     /label=USER linker
                     /note="USER linker"
     terminator      7048..7080
                     /note="BBa_B1002"
     terminator      complement(7082..7120)
                     /note="BBa_B1006"
     modified_base   complement(7121..7139)
                     /label=USER Junction
                     /note="USER Junction"
     misc_feature    7140..7222
                     /label=gRNA backbone
                     /note="/dnas_title=gRNA backbone"
                     /note="/vntifkey=21"
     primer_bind     complement(7223..7242)
                     /label=Target DNA
                     /note="/created_by=User"
     primer_bind     complement(7227..7282)
                     /label=WT185
     primer_bind     complement(7236..7268)
                     /label=WT185
     misc_feature    7243
                     /label=TSS
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(7249..7254)
                     /label=-10 box
                     /note="/created_by=User"
     primer_bind     7271..7323
                     /label=WT186
     misc_feature    complement(7272..7277)
                     /label=-33 box
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(7278..7296)
                     /label=Lac operator
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     terminator      complement(7297..7338)
                     /label=tetA/orfL bidirectional terminator
     modified_base   7339..7351
                     /label=USER junction
                     /note="USER junction"
     CDS             complement(7352..8143)
                     /codon_start=1
                     /label=aadA, streptomycin adenylyltransferase
                     /note="/dnas_title=aadA, streptomycin adenylyltransferase"
                     /note="/modified_by=User"
                     /note="/vntifkey=4"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(7355..7374)
                     /label=WT040 aadA end FP sequencing
     primer_bind     7608..7617
                     /label=WT071 seq RepA 300 FP
     primer_bind     8126..8143
                     /label=WT050 seq
     promoter        8144..8228
                     /note="aadA promoter"
     primer_bind     complement(8208..8242)
                     /label=WT108 revised WT081
     primer_bind     8229..23
                     /label=WT080
     modified_base   8229..8242
                     /label=USER junction
                     /note="USER junction"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt attctagagc acagctccat cgaatggcca gatgatccct
     1801 atcagtgata gagattgaca ctctatcatt gatagagtta ttttaccact ccctatcagt
     1861 gatagagaaa agtgaaatga atagttcgac aaaaatctag acctgcaggt gcagtaagga
     1921 ggaaaaaaaa atgtcttctg aaaccggtcc ggttgcggtt gacccgaccc tgcgtcgtcg
     1981 tatcgaaccg cacgaattcg aagttttctt cgacccgcgt gaactgcgta aagaaacctg
     2041 cctgctgtac gaaatcaact ggggtggtcg tcactctatc tggcgtcaca cctctcagaa
     2101 caccaacaaa cacgttgaag ttaacttcat cgaaaaattc accaccgaac gttacttctg
     2161 cccgaacacc cgttgctcta tcacctggtt cctgtcttgg tctccgtgcg gtgaatgctc
     2221 tcgtgcgatc accgaattcc tgtctcgtta cccgcacgtt accctgttca tctacatcgc
     2281 gcgtctgtac caccacgcgg acccgcgtaa ccgtcagggt ctgcgtgacc tgatctcttc
     2341 tggtgttacc atccagatca tgaccgaaca ggaatctggt tactgctggc gtaacttcgt
     2401 taactactct ccgtctaacg aagcgcactg gccgcgttac ccgcacctgt gggttcgtct
     2461 gtacgttctg gaactgtact gcatcatcct gggtctgccg ccgtgcctga acatcctgcg
     2521 tcgtaaacag ccgcagctga ccttcttcac catcgcgctg cagtcttgcc actaccagcg
     2581 tctgccgccg cacatcctgt gggcgaccgg tctgaaatcc ggtagcgaaa caccggggac
     2641 ttcagaatcg gccaccccgg agtctgataa gaaatactca ataggcttag ctatcggcac
     2701 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa
     2761 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt
     2821 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac
     2881 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt
     2941 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca
     3001 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc
     3061 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt
     3121 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga
     3181 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa
     3241 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc
     3301 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa
     3361 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa
     3421 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga
     3481 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc
     3541 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac
     3601 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac
     3661 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga
     3721 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta
     3781 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact
     3841 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca
     3901 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt
     3961 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg
     4021 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat
     4081 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga
     4141 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt
     4201 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg
     4261 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt
     4321 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga
     4381 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     4441 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     4501 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     4561 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     4621 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     4681 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     4741 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     4801 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     4861 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     4921 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     4981 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
     5041 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     5101 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     5161 aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga
     5221 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
     5281 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     5341 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     5401 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     5461 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     5521 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     5581 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     5641 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     5701 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     5761 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     5821 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     5881 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     5941 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa
     6001 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     6061 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     6121 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc
     6181 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga
     6241 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     6301 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     6361 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     6421 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     6481 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     6541 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     6601 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     6661 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac
     6721 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgacagcg gcggttcaat
     6781 gacaaatctg tcggatatca tcgaaaaaga aaccggtaaa cagctggtta tccaagaaag
     6841 tattttaatg cttcccgaag aagtcgaaga ggtgattggc aacaaacccg aaagtgatat
     6901 tctcgtgcat actgcgtatg atgagtcgac cgatgaaaac gtcatgctgc tgacctccga
     6961 cgcgcccgag tataaaccgt gggctttggt tatccaggat agcaacggtg aaaataagat
     7021 taaaatgtta taaacggagc caatgtacgc aaaaaccccg cttcagcggg gttttttcgc
     7081 caaaaaaaac cccgccctgt caggggcggg gttttttttt atgttgaaaa tctccttcta
     7141 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
     7201 ttgctatttc tagctctaaa acccggcaag ctgcccgtgc ccgtgctcag tatcttgtta
     7261 tccgctcaca atgtcaattg ttatccgctc acaattaaat ataatgaccc tcttgataac
     7321 ccaagagggc attttttaat gcccatggcg tttatttgcc gactaccttg gtgatctcgc
     7381 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     7441 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     7501 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     7561 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     7621 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     7681 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     7741 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     7801 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     7861 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     7921 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     7981 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     8041 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     8101 ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc ctttttcaat
     8161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8221 agaaaaatag gccaaatgcc gt
//
