LOCUS       Exported                2280 bp ds-DNA     circular UNA 26-JUN-2017
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2018 from SnapGene 4.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        67..330
                     /label=hU6 prom
     primer_bind     complement(279..321)
                     /label=WT605
     primer_bind     complement(309..362)
                     /label=WT605
     primer_bind     complement(316..350)
                     /label=WT605
     misc_feature    332..351
                     /label=TCATCCTCCTGACAATCGAT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TCATCCTCCTGACAATCGAT"
     misc_feature    332..351
                     /label=CCR5-f
                     /label=nonstandard type: motif
                     /note="/created_by=wtang"
                     /note="/modified_by=wtang"
     misc_feature    complement(332..344)
                     /label=CCR5_O
                     /label=nonstandard type: motif
                     /note="/created_by=wtang"
     misc_feature    complement(332..341)
                     /label=CCR5_P
                     /label=nonstandard type: motif
                     /note="/created_by=wtang"
     misc_feature    344..351
                     /label=CCR5-g
                     /label=nonstandard type: motif
                     /note="/created_by=wtang"
                     /note="/modified_by=wtang"
     misc_feature    complement(346..351)
                     /label=CCR5-e
                     /label=nonstandard type: motif
                     /note="/created_by=wtang"
     misc_feature    complement(352..434)
                     /label=gRNA backbone
                     /note="/dnas_title=gRNA backbone"
                     /note="/vntifkey=21"
     primer_bind     357..385
                     /label=WT523
     misc_feature    complement(516..1135)
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(1290..2150)
                     /label=amp marker
                     /label=nonstandard type: Marker
     promoter        complement(2192..2220)
                     /label=amp prom
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcacacttgt caccacccca agttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      421 tcggtgcttt ttttaagctt gggccgctcg aggtacctct ctacatatga catgtgagca
      481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
