LOCUS       pPtGE34                12392 bp ds-DNA     linear       14-FEB-2018
DEFINITION  pKS diaCas9_sgRNA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(135..441)
                     /locus_tag="f1_origin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(474..622)
                     /locus_tag="lacZ_a"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    588..610
                     /locus_tag="M13_pUC_fwd_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        603..619
                     /locus_tag="M13_forward20_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        626..644
                     /locus_tag="T7_promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4933..4998
                     /locus_tag="3xFLAG"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5005..5028
                     /locus_tag="SV40 NLS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(10157..10173)
                     /locus_tag="pBluescriptKS_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(10200..10219)
                     /locus_tag="T3_promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(10236..10254)
                     /locus_tag="M13_reverse_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(10253..10275)
                     /locus_tag="M13_pUC_rev_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(10289..10318)
                     /locus_tag="lac_promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(10627..11246)
                     /locus_tag="pBR322_origin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(11401..12261)
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(12303..12331)
                     /locus_tag="AmpR_promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5050..9201
                     /note="diaCas9"
                     /locus_tag="diaCas9"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4474..4917
                     /note="FcpB Promoter"
                     /locus_tag="FcpB Promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        9709..9733
                     /note="gRNA stuffer"
                     /locus_tag="gRNA stuffer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9810..10147
                     /note="U6 Terminator"
                     /locus_tag="U6 Terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9434..9713
                     /note="U6 Promoter"
                     /locus_tag="U6 Promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9212..9427
                     /note="FcpA Terminator"
                     /locus_tag="FcpA Terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        9734..9749
                     /note="crRNA"
                     /locus_tag="crRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        9750..9809
                     /note=" tracrRNA"
                     /locus_tag=" tracrRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    658..1428
                     /note="oriT"
                     /locus_tag="oriT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      1134..1153
                     /pair=""
                     /primer="CTTGGTTTCATCAGCCATCC"
                     /current=0
                     /locus_tag="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      complement(1628..1649)
                     /pair=""
                     /primer="TTAGGTACCAGCTTTTGTTCCC"
                     /current=0
                     /locus_tag="PCR_primer(1)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3125..4473
                     /note="CAH"
                     /locus_tag="CAH"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      complement(3339..3358)
                     /pair=""
                     /primer="CACAGGATTTTCGTGTGTGG"
                     /current=0
                     /locus_tag="PCR_primer(2)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      597..676
                     /pair=""
                     /primer="GACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG
                     CGAATTGGAGCTGATCGTCTTGCCTTGCTCG"
                     /current=0
                     /locus_tag="PCR_primer(3)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      complement(4451..4530)
                     /pair=""
                     /primer="TCCAAAAGTTCTAGCTTTGGATGTTGAACTTTTATACACCATGAATAGG
                     CGAGATTGCATAAGAACACCTTTGGTGGAGG"
                     /current=0
                     /locus_tag="PCR_primer(4)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     insert          1650..2249
                     /type="40SRPS8 Promoter"
                     /locus_tag="insert"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2250..2624
                     /note="shble"
                     /locus_tag="shble"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      2250..2269
                     /pair=""
                     /primer="2548"
                     /current=0
                     /locus_tag="PCR_primer(5)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      complement(2226..2285)
                     /pair=""
                     /primer="GGTGAGCACCGGAACGGCACTGGTCAACTTGGCCATGGTATTCTATTCT
                     CTGATTCCTTC"
                     /current=0
                     /locus_tag="PCR_primer(6)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      complement(2334..2351)
                     /pair=""
                     /primer="CACGAAGTCCCGGGAGAA"
                     /current=0
                     /locus_tag="PCR_primer(7)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      2414..2433
                     /pair=""
                     /primer="CCAGGTGGTGCCGGACAACA"
                     /current=0
                     /locus_tag="PCR_primer(8)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      2254..2273
                     /pair=""
                     /primer="CCAAGTTGACCAGTGCCGTT"
                     /current=0
                     /locus_tag="PCR_primer(9)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2625..3124
                     /note="40SRPS Terminator"
                     /locus_tag="40SRPS Terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      2555..2574
                     /pair=""
                     /primer="GGGGCGGGAGTTCGCCCTGC"
                     /current=0
                     /locus_tag="PCR_primer(10)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      1609..1668
                     /pair=""
                     /primer="GAAATTAACCCTCACTAAAGGGAACAAAAGCTGGTACCTAACCCTGCGA
                     TAGACCTTTTC"
                     /current=0
                     /locus_tag="PCR_primer(11)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      complement(5090..5109)
                     /pair=""
                     /primer="GATGACGGCCCATCCAACAG"
                     /current=0
                     /locus_tag="PCR_primer(12)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     PCR_primer      9639..9658
                     /pair=""
                     /primer="GACACGCCAAGTATTCATTG"
                     /current=1
                     /locus_tag="PCR_primer(13)"
                     /ApEinfo_label="PCR_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT
      481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
      541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
      601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTGAT
      661 CGTCTTGCCT TGCTCGTCGG TGATGTACTT ACAGCTCGAA GTGCCTCTTC TTGATGGAGC
      721 GCATGGGGAC GTGCTTGGCA ATCACGCGCA CCCCCCGGCC GTTTTAGCGG CTAAAAAAGT
      781 CATGGCTCTG CCCTCGGGCG GACCACGCCC ATCATGACCT TGCCAAGCTC GTCCTGCTTC
      841 TCTTCGATCT TCGCCAGCAG GGCGAGGATC GTGGCATCAC CGAACCGCGC CGTGCGCGGG
      901 TCGTCGGTGA GCCAGAGTTT CAGCAGGCCG CCCAGGCGGC CCAGGTCGCC ATTGATGCGG
      961 GCCAGCTCGC GGACGTGCTC ATAGTCCACG ACGCCCGTGA TTTTGTAGCC CTGGCCGACG
     1021 GCCAGCAGGT AGGCCGACAG GCTCATGCCG GCCGCCGCCG CCTTTTCCTC AATCGCTCTT
     1081 CGTTCGTCTG GAAGGCAGTA CACCTTGATA GGTGGGCTGC CCTTCCTGGT TGGCTTGGTT
     1141 TCATCAGCCA TCCGCTTGCC CTCATCTGTT ACGCCGGCGG TAGCCGGCCA GCCTCGCAGA
     1201 GCAGGATTCC CGTTGAGCAC CGCCAGGTGC GAATAAGGGA CAGTGAAGAA GGAACACCCG
     1261 CTCGCGGGTG GGCCTACTTC ACCTATCCTG CCCGGCTGAC GCCGTTGGAT ACACCAAGGA
     1321 AAGTCTACAC GAACCCTTTG GCAAAATCCT GTATATCGTG CGAAAAAGGA TGGATATACC
     1381 GAAAAAATCG CTATAATGAC CCCGAAGCAG GGTTATGCAG CGGAAGATGG CCATTCGCCA
     1441 TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG
     1501 CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG
     1561 TCACGACGTT GTAAAACGAC GGCCAGTGAC CATGATTACG CCAAGCTCGA AATTAACCCT
     1621 CACTAAAGGG AACAAAAGCT GGTACCTAAC CCTGCGATAG ACCTTTTCCA AACTCACGCA
     1681 GTCCAAGAAA ACAAAGGGGT GAGAAGTATA CGCACCTTTC GGTTTCGGCA TAATTCTTAA
     1741 ACTCTTGTGG TCACTTTCTT GTGAAGAAGC TAGGGGCACT CGTTTTCCCT CAGAGCCTGC
     1801 AAACACAAAA TTCCTGCAGT CAATTGTCCC AACACTCGGC AAACCGTATG CGCAAGCAAC
     1861 GATGCGCAGA AGGCCGTGGA TGGATGGCGA CTCGCGATAT GGCTTCTTGG GTCGCCAGTG
     1921 TGGTACGTCC GGCGTATGTC AATACGCGAA TTCGGACGAC TGGCATCTCT AGGAGGAGGA
     1981 TTCCTTCTTT TATGACATGT TTATTTTATA TACATTGATG CTTTCCGACA GTCGGAAGTA
     2041 ATAAATGAAT TTATTTCAAG ACTACCTATA CTCCTTTGAC TTGTTCGACT AATCTTACCG
     2101 CTTACTAAAA TCTCGAAATC ACGCTTGACC TCTCGCACGA AAATTTTTGC TGCTGGACGC
     2161 TACACACTCG GCCCAATTCT TCTCGGTCCT CGTCGTCGCA ATTGTCGTTG CGTTGATCTT
     2221 GCACCGAAGG AATCAGAGAA TAGAATACCa tggccaagtt gaccagtgcc gTTCCGGTGC
     2281 TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC
     2341 GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA
     2401 GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC
     2461 TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG
     2521 GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC
     2581 CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGAAGATAA GAATATCTCA
     2641 TTGTGAACAT CTAATGATTT ACCAATTTTA TTCTTTGTTT ACAGTTAGAC GCCAGTAATT
     2701 GTGCTGTTTC TCTCAAGTCT GTGTCAATAC AAACTACGAA ACTTGGCAAT TTTTCTCTTG
     2761 AATATGAGCA CGAGATTGAA ACGCACAAAG GAAATTAGTT TCCATCCTTT GACAAAGTTT
     2821 GTTGCTGTTT AGAGAACAGA TGTCAAAATT AACGTGCCAT GGAATTGAAC CATGGTGCGC
     2881 TATCCCCAAA TCACGTCGTT TGACCTCGTC ACATTTAAGG TATATCAAGC ATATTCACTT
     2941 ATATCTTGAC ATCCTCCCTG CTTGATATTC CTTTGCCCTT GAGCCATCTT CCCCACACGC
     3001 TGGAGATGAC CCCTGCCCCT CCTTTCTTTA TCCACCCGAG TAAGGTGTCG ACAAGTCACT
     3061 TTGCCCCTGA GCCATCCTCC CCACTCAGGA GATGACCCCT ACCCCTTTCT TCTTTATCCA
     3121 CCCCcacgtg ctataaaaat aattataatt taaatttttt aatataaata tataaattaa
     3181 aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa
     3241 agactctagg gggatcgcca acaaatacta ccttttacct tgctcttcct gctctcaggt
     3301 attaatgccg aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat
     3361 tttacatttt acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa
     3421 taaatatata tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt
     3481 ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac
     3541 ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg
     3601 cgcgtgtaag ttacaggcaa gcgatcctag tacactctat atttttttat gcctcggtaa
     3661 tgattttcat tttttttttc cacctagcgg atgactcttt ttttttctta gcgattggca
     3721 ttatcacata atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta
     3781 aaaaatgagc aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag
     3841 cgtattacaa atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg
     3901 atagagcact cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa
     3961 tcgcaagtga ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg
     4021 gccaagcatt ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac
     4081 catcacacca ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctaggg
     4141 gccgtgcgtg gagtaaaaag gtttggatca ggatttgcgc ctttggatga ggcactttcc
     4201 agagcggtgg tagatctttc gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg
     4261 gagaaagtag gagatctctc ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag
     4321 gctagcagaa ttaccctcca cgttgattgt ctgcgaggca agaatgatca tcaccgtagt
     4381 gagagtgcgt tcaaggctct tgcggttgcc ataagagaag ccacctcgcc caatggtacc
     4441 aacgatgttc cctccaccaa aggtgttctt atgCAATCTC GCCTATTCAT GGTGTATAAA
     4501 AGTTCAACAT CCAAAGCTAG AACTTTTGGA AAGAGAAAGA ATATCCGAAT AGGGCACGGC
     4561 GTGCCGTATT GTTGGAGTGG ACTAGCAGAA AGTGAGGAAG GCACAGGATG AGTTTTCTCG
     4621 AGACACATAG CTTCAGCGTC GTGTAGGCTA GGCAGAGGTG AGTTTTCTCG AGACATACCT
     4681 TCAGCGTCGT CTTCACTGTC ACAGTCAACT GACAGTAATC GTTGATCCGG AGAGATTCAA
     4741 AATTCAATCT GTTTGGACCT GGATAAGACA CAAGAGCGAC ATCCTGACAT GAACGCCGTA
     4801 AACAGCAAAT CCTGGTTGAA CACGTATCCT TTTGGGGGCC TCCGCTACGA CGCTCGCTCC
     4861 AGCTGGGGCT TCCTTACTAT ACACAGCGCG CATATTTCAC GGTTGCCAGA AGTCAAGATG
     4921 GCCTCTAGAA TGGACTATAA GGACCACGAC GGAGATTACA AGGATCATGA TATTGATTAC
     4981 AAAGACGATG ATGATAAAAT GGCCCCAAAG AAGAAGCGGA AAGTCGGTAT ACACGGAGTC
     5041 CCAGCAGCCG ACAAGAAGTA CTCGATTGGA TTGGACATCG GCACCAACTC TGTTGGATGG
     5101 GCCGTCATCA CGGACGAGTA CAAGGTGCCC AGCAAGAAAT TCAAAGTACT GGGAAACACT
     5161 GACCGCCATT CGATCAAGAA GAATTTGATT GGAGCTTTGC TGTTTGACAG CGGCGAAACA
     5221 GCGGAAGCCA CGCGGCTTAA ACGAACCGCG AGACGAAGAT ATACGCGACG GAAGAACCGT
     5281 ATCTGCTATC TACAAGAGAT TTTCTCGAAT GAGATGGCAA AAGTCGATGA CTCTTTCTTT
     5341 CACCGATTGG AAGAATCCTT CCTCGTTGAA GAGGATAAGA AACATGAGCG GCACCCGATA
     5401 TTTGGAAACA TTGTGGACGA GGTGGCTTAC CATGAGAAAT ACCCGACTAT TTACCACCTG
     5461 CGAAAGAAAT TGGTGGACTC GACGGACAAA GCGGATCTTC GCCTAATCTA TTTGGCGCTA
     5521 GCACATATGA TTAAGTTTCG CGGCCACTTC TTGATCGAAG GCGACTTGAA TCCGGACAAC
     5581 AGTGATGTTG ACAAACTGTT TATTCAACTT GTACAAACTT ACAACCAGCT CTTTGAAGAA
     5641 AATCCCATTA ACGCTTCGGG AGTGGACGCG AAAGCCATCC TTTCTGCCCG ACTCAGCAAG
     5701 AGCCGACGCC TAGAAAATTT GATTGCGCAA CTTCCCGGCG AGAAAAAGAA TGGACTTTTT
     5761 GGAAATCTTA TTGCTTTGTC GCTGGGCCTT ACGCCGAACT TTAAGAGCAA CTTCGACCTT
     5821 GCGGAAGATG CCAAATTGCA ACTGAGTAAA GACACATATG ACGACGACCT CGACAATCTT
     5881 TTGGCACAAA TAGGAGATCA GTACGCCGAC CTCTTTTTGG CGGCTAAGAA CTTGTCCGAC
     5941 GCCATTCTGT TGTCGGACAT CCTTCGAGTT AATACGGAGA TTACTAAGGC GCCCTTGAGC
     6001 GCCTCTATGA TCAAAAGATA CGACGAGCAT CACCAAGACC TGACCCTCCT TAAAGCTCTC
     6061 GTCCGGCAAC AGCTGCCTGA GAAGTACAAA GAGATTTTCT TTGATCAAAG CAAAAACGGA
     6121 TACGCGGGCT ACATTGACGG GGGAGCCTCG CAAGAAGAGT TCTACAAGTT TATTAAACCG
     6181 ATCCTGGAAA AGATGGACGG CACGGAGGAA TTACTCGTGA AACTTAACCG AGAGGACCTT
     6241 TTGCGTAAGC AACGGACTTT CGACAACGGA TCTATTCCGC ACCAAATCCA TCTCGGAGAA
     6301 TTGCACGCAA TTCTGCGCCG GCAAGAAGAT TTTTATCCAT TTCTCAAGGA CAACCGGGAA
     6361 AAGATTGAGA AAATCTTAAC CTTCCGCATA CCCTACTACG TCGGCCCTTT GGCCAGGGGA
     6421 AATTCGAGAT TTGCCTGGAT GACGCGAAAG AGTGAGGAAA CTATTACTCC CTGGAATTTC
     6481 GAAGAAGTGG TAGACAAAGG AGCTTCCGCG CAATCCTTTA TCGAGCGCAT GACCAACTTC
     6541 GATAAGAACC TCCCGAATGA GAAAGTCCTA CCCAAGCATT CGTTGCTCTA CGAATACTTT
     6601 ACGGTGTATA ATGAGCTGAC TAAAGTCAAA TACGTTACAG AGGGAATGCG AAAACCGGCT
     6661 TTCCTCTCGG GAGAGCAGAA AAAGGCCATA GTCGATCTTC TCTTTAAAAC GAACCGTAAA
     6721 GTTACCGTGA AACAACTAAA AGAGGACTAC TTCAAGAAAA TCGAATGCTT TGACTCCGTT
     6781 GAAATTTCCG GCGTTGAAGA TCGGTTCAAT GCCTCCTTGG GAACATACCA CGATCTCCTG
     6841 AAAATTATCA AAGACAAGGA CTTTCTTGAC AATGAGGAAA ACGAGGACAT TCTTGAAGAT
     6901 ATTGTCTTGA CGCTAACACT TTTTGAGGAT CGAGAGATGA TTGAGGAACG CCTTAAAACC
     6961 TATGCGCATC TGTTCGACGA CAAAGTTATG AAGCAATTGA AGCGCCGGAG ATATACGGGA
     7021 TGGGGAAGGT TGAGTCGGAA ACTGATAAAT GGCATACGCG ATAAGCAGTC CGGCAAAACA
     7081 ATTCTCGATT TCTTGAAGTC CGACGGATTT GCGAATCGAA ACTTCATGCA ACTGATCCAT
     7141 GACGATTCGT TGACCTTTAA AGAGGACATT CAAAAAGCCC AAGTATCCGG ACAAGGAGAT
     7201 TCTTTGCACG AGCATATTGC GAATCTTGCT GGAAGTCCGG CTATTAAAAA GGGCATTCTC
     7261 CAGACAGTAA AGGTCGTGGA TGAACTCGTT AAAGTTATGG GCCGACACAA ACCCGAGAAC
     7321 ATAGTCATCG AAATGGCGAG AGAGAACCAG ACCACCCAAA AGGGACAGAA AAATTCACGC
     7381 GAGCGAATGA AGCGGATTGA AGAGGGGATC AAAGAGCTTG GCAGCCAAAT TCTGAAAGAA
     7441 CACCCGGTTG AAAACACCCA ATTGCAGAAC GAGAAGCTCT ACCTGTACTA TCTTCAAAAT
     7501 GGGCGTGATA TGTACGTCGA TCAAGAATTG GACATCAACC GGCTATCCGA TTACGATGTG
     7561 GACCATATTG TTCCTCAGTC GTTTCTCAAA GACGACTCCA TAGACAATAA GGTTCTAACG
     7621 AGAAGCGACA AGAACCGCGG CAAATCAGAC AACGTCCCCT CCGAGGAAGT AGTTAAGAAA
     7681 ATGAAGAATT ACTGGCGCCA ATTGCTGAAC GCTAAGCTCA TTACCCAAAG AAAGTTTGAC
     7741 AATTTGACGA AAGCCGAGCG AGGCGGGCTC TCGGAACTGG ATAAGGCCGG CTTCATTAAA
     7801 AGACAATTGG TGGAAACGCG ACAAATCACA AAGCACGTCG CACAGATTCT CGACTCCCGC
     7861 ATGAACACTA AGTATGACGA GAATGACAAA CTAATCCGGG AAGTCAAAGT GATTACCTTG
     7921 AAGTCCAAGC TGGTTTCCGA TTTTCGTAAA GATTTTCAGT TTTACAAAGT TCGCGAGATC
     7981 AACAACTACC ATCACGCGCA CGACGCTTAC TTGAACGCTG TCGTGGGAAC GGCCTTGATT
     8041 AAAAAATACC CTAAGCTGGA AAGCGAGTTC GTTTACGGCG ATTACAAGGT GTATGACGTA
     8101 CGCAAAATGA TCGCGAAGTC AGAGCAGGAA ATTGGCAAAG CTACTGCGAA GTACTTCTTT
     8161 TATAGCAACA TAATGAATTT TTTCAAAACG GAGATTACCC TCGCTAACGG CGAGATTCGC
     8221 AAGCGCCCTC TTATCGAGAC AAACGGCGAA ACGGGGGAGA TTGTCTGGGA TAAAGGCCGC
     8281 GATTTTGCGA CTGTGCGGAA AGTCTTGTCG ATGCCGCAAG TGAATATAGT CAAAAAGACG
     8341 GAGGTGCAAA CAGGCGGCTT TTCGAAAGAA TCTATCCTTC CCAAAAGGAA CTCTGATAAG
     8401 TTGATTGCGC GAAAAAAGGA TTGGGACCCT AAGAAGTATG GCGGCTTTGA CAGCCCGACC
     8461 GTAGCATATT CTGTCCTTGT GGTGGCCAAA GTCGAAAAGG GCAAATCCAA GAAACTGAAG
     8521 AGTGTGAAAG AATTGCTTGG GATTACGATC ATGGAAAGAT CATCTTTTGA GAAGAATCCC
     8581 ATCGACTTTC TCGAAGCTAA AGGCTACAAA GAAGTGAAAA AGGACCTGAT TATCAAGTTG
     8641 CCTAAGTACT CCTTGTTTGA GCTGGAAAAT GGCCGTAAGC GAATGCTCGC TTCTGCCGGC
     8701 GAACTCCAGA AGGGAAACGA ATTGGCGCTG CCCTCCAAAT ATGTTAACTT TCTTTACCTT
     8761 GCCTCGCACT ATGAGAAGCT CAAGGGCTCC CCCGAGGATA ATGAGCAAAA ACAACTGTTT
     8821 GTAGAACAGC ATAAGCACTA CTTGGACGAG ATCATTGAGC AAATTAGCGA GTTCTCCAAG
     8881 CGAGTCATCC TTGCCGACGC TAATCTGGAC AAAGTCCTGT CCGCCTACAA TAAGCACCGC
     8941 GATAAGCCAA TCCGAGAGCA AGCGGAGAAT ATTATCCACT TGTTTACGCT CACCAATCTT
     9001 GGAGCCCCTG CCGCCTTTAA GTACTTTGAC ACCACCATCG ACCGCAAGAG GTACACCAGC
     9061 ACCAAAGAAG TCTTGGACGC CACCCTCATT CACCAATCAA TCACCGGCTT GTACGAGACA
     9121 CGCATCGACC TGTCTCAACT CGGAGGCGAC AAAAGGCCGG CGGCCACGAA AAAGGCCGGC
     9181 CAGGCAAAAA AGAAAAAGTA ATCTGGATCC CTACCTCGAC TTTGGCTGGG ACACTTTCAG
     9241 TGAGGACAAG AAGCTCCAGA AGCGTGCTAT CGAACTCAAC CAGGGACGTG CGGCACAAAT
     9301 GGGCATCCTT GCTCTCATGG TGCACGAACA GTTGGGAGTC TCTATCCTTC CTTAAAAATT
     9361 TAATTTTCAT TAGTTGCAGT CACTCCGCTT TGGTTTCACA GTCAGGAATA ACACTAGCTC
     9421 GTCTTCACTG CAGGTTGGCT CGGAAGTTGG TGTTGACGGT GAGCTGGAAA TTGGTTGTCG
     9481 GTCACTGCTA GCGAGAAGAA AACGGAGGAC AGAAGGAAGT GAAACTCGGT TCGTTCTCGA
     9541 CAGCCTCACT GTCAATATGC TCATTTTCAA TCCTTAGCGC TTTTAATGTC GAATTGACGG
     9601 TAAATTGAAT AGGATCTATA ATATCTACAA GGTACTTTGA CACGCCAAGT ATTCATTGTT
     9661 AGTCAACAAT ATTTTAGAGC TTTATAAGGT CAAAAAACAC CTTCAAAGTC GAGgagaccG
     9721 AGAGAGggtc tcAgtttTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC
     9781 AACTTGAAAA AGTGGCACCG AGTCGGTGCT TTTTTTTAGA ACCGCTCACC CATGCTATCG
     9841 TATGTCATTT ACATTGACAA ACTGCTATGA ATGCGTTTGC GATAACTTCA GCTCGCTGAA
     9901 GCCCTAGACA TTTCGTTTGA CTAAATTGTT TTCCGGTTGC TTTACTACCG ACCGGTCTGC
     9961 GGGTAGCATG TCTTGGCTTT TGCGAGTTCG AGAGAATTTA TGGACAAGTG CGTTTACAGT
    10021 TTGGAACACG AGTGTGAGTG AGCGGAATTG GCTATCTATA ACATTCTTAT TTCAGGAGGC
    10081 TGGTGATTAG CAGAATTTTC AAAGATCTCG AATCTAAAAT CGCCATGGGC TCGACGAACT
    10141 TTTCTGCAAG CTTATCGATA CCGTCGACCT CGAGGGGGGG CCCGGTACCA GCTTTTGTTC
    10201 CCTTTAGTGA GGGTTAATTT CGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
    10261 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
    10321 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
    10381 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
    10441 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
    10501 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
    10561 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
    10621 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
    10681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
    10741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
    10801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
    10861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
    10921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
    10981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
    11041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
    11101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
    11161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
    11221 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
    11281 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
    11341 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
    11401 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
    11461 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
    11521 CAGTGCTGCA ATGATACCGC GAGAGCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
    11581 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
    11641 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
    11701 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
    11761 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
    11821 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
    11881 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
    11941 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
    12001 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
    12061 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
    12121 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
    12181 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
    12241 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
    12301 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
    12361 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TG  
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