LOCUS Exported 10169 bp ds-DNA circular SYN 15-FEB-2018 DEFINITION . ACCESSION . VERSION . KEYWORDS pCFJ150 GFP_dpiRNA_CDK-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10169) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Feb 15, 2018 from SnapGene 2.3.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10169 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 2329..3756 /note="LGII R" CDS join(4233..4403,4455..4608,4660..4823,4875..5105) /codon_start=1 /note="GFP_dPiRNA" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYKAS" CDS join(5106..5282,5333..5539,5588..5743,5795..6121, 6169..6297) /codon_start=1 /note="Feature 14" /translation="DPIREGEVAHEGDSVYTLNDFTKLEKIGEGTYGVVYKGKNRRTNA MVAMKKIRLESEDEGVPSTAVREISLLKELQHPNVVGLEAVIMQENRLFLIFEFLSFDL KRYMDQLGKDEYLPLETLKSYTFQILQAMCFCHQRRVIHRDLKPQNLLVDNNGAIKLAD FGLARAIGIPIRVYTHEVVTLWYRAPEILMGAQRYSMGVDMWSIGCIFAEMATKKPLFQ GDSEIDELFRIFRVLGTPTELEWNGVESLPDYKATFPKWRENFLRDKFYDKKTGKHLLD DTAFSLLEGLLIYDPSLRLNAKKALVHPYFDNMDTSKLPAGNYRGELELF" misc_feature complement(6994..7100) /note="unc-119 cb4" misc_feature complement(7152..7529) /note="unc-119 cb3" misc_feature complement(7573..7684) /note="unc-119 cb2" /note="unc-119cb 2" misc_feature complement(7783..7839) /note="unc-119 cb1" /note="unc-119cb 1" misc_feature 8828..10163 /note="LGII L" ORIGIN 1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc 121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agcacgtgtg tttttgaaca 2341 cggcgatatg tatctgtaga tcatccccaa ttttcaacaa tgaatctaaa tactgaactt 2401 tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc gattttccga ttatacccaa 2461 aatatctcag ctcaatgagc acctataaaa atgtaaaaat aaagaaagta gtcgaaccat 2521 attttgtaaa ggctaaaaaa ctgaataaat ttttatccat ccagcgcggc gttacaatcc 2581 gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca gtctgtttgc gcgacatcga 2641 tctggcaaaa atcctaaaaa cgactatgcg tggctcactt tatattaata tgaaaaatac 2701 aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag aagtggaggc cttgttgtcg 2761 atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa cgctctcaat cttggatatg 2821 atataaattc tggataagat atgatggata tgatctatgt ttgatagagt gcatttgaaa 2881 ggataacggc cctgcagaca tttttgaaaa aataacaaac atttaatttt ctcactcgtt 2941 taggctattc ccctattttt gatattcctt cgcacatatg aaaactactt tttttcgaaa 3001 ctgttaactc cagaatttat aaatctatag cccttacttg attatttatt atcatggtta 3061 ctcacctcat gtctctttct cttttcaaga agtcttcgtt catatctgtt gtctctttcc 3121 tccttttgaa tctctgcttt gctcttcgcc attgtttcct gaaaataatg taacttgaat 3181 tgtgtaatat acttttttaa tttgaatttg gcttgtaacg cggaatcact acgtgcggga 3241 tcatttctta ctagaaaacc cagaaaatgc catatttcac ttatctcggg gtcatttcta 3301 attagaaaag ctacaaaaac catttctaat tacggtgact ttagaactgg tcgaaatagc 3361 taattagaaa tgggtttttg ctaattagag gtgactctat gttatctgag taagcctcgt 3421 ttgtatgtaa actcacagta ctaataaatg caagacaccc gggtttgtct agatatgaaa 3481 taattgaaat atcaattctg acagacaata atggtaatct tgataaggag ttccacgccc 3541 aggagaacac gttagttttc ttgtttttga ttgcgtgcgt tattttggag aaaaactcga 3601 ttttttacaa aataattttt tgaaaggaac actgttcaat aagttttgtc ttttttctca 3661 gttgtgatac ggttttttat tcttttttgt agttatacag aagaccgtta cgaaacagac 3721 tgatatcgaa acaaacactg atcttacttg cacttataat acgactcact agtgggcaga 3781 tctgggtacc ggtggtggct ccggtattga gggacgcggg tccatgggat atcggggatc 3841 cgaattctgt acaggccttg gcgcgcctgc aggcgagctc cgtcgacaag cttgcggccg 3901 cggaagcctc ataaagtaga acaaatcaag tttatgatta ttttgagtag ttatttttat 3961 attttccggc ctgtatttat tctcttttct gcacagtttt gcgttaaatt ttatcatatg 4021 aatgcaatgt ttcacaacaa tccttctcag cgcgtactct ccttttcgga ataaattgtc 4081 atcaaaggtg catgcctttt cactaagcaa tggtctcgca ccgactgtcc ccccttttcg 4141 gcggcagtgg aaatgattca aaattacacg cttacgcctt ttctatcgtt gtattacaat 4201 tgttctgaca aaattcattt ccaattttag ttatgagtaa aggagaagaa cttttcactg 4261 gagttgtccc aatccttgtt gaattagatg gtgatgttaa tgggcacaaa ttttccgtca 4321 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atctgcacta 4381 ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt 4441 atttaaattt tcagccaaca cttgtcacta ctctcactta tggtgttcaa tgcttctcga 4501 gatacccaga tcatatgaaa cagcatgact tcttcaagag tgccatgccc gaaggttatg 4561 tacaggaaag aacaatattt ttcaaagacg acgggaacta caagacacgt aagtttaaac 4621 agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc aagtttgaag 4681 gagacaccct tgtcaataga atcgagttaa aaggtattga ttttaaagaa gacggcaaca 4741 ttcttggcca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca 4801 aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact aactaatctg 4861 atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc gtacaactag 4921 cagaccatta tcaacaaaat actccaatag gagatggccc tgtcctttta ccagacaacc 4981 attacctgtc cacacaatct gccctttcta aagaccccaa cgaaaagaga gaccacatgg 5041 tccttcttga gttcgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaag 5101 catctgatcc tattcgcgaa ggagaagtgg cccacgaggg agattcggtt tacacactca 5161 acgatttcac gaagctcgaa aaaatcggcg aaggaacata cggagtcgtc tacaaaggca 5221 aaaacagaag aaccaatgcc atggttgcta tgaagaaaat cagattggaa agtgaagatg 5281 aggtatttga attttgtagc agtttcgttt tttcaaacaa taattcttat agggtgttcc 5341 atcaacggct gtgcgagaga tcagcttgct caaagagctc cagcatccga atgttgttgg 5401 attggaagcg gtcattatgc aggagaaccg gcttttcctg atcttcgaat tcctgtcttt 5461 cgacttgaaa cgatacatgg atcagttggg aaaagatgaa taccttccgc tcgaaactct 5521 taaaagttac acattccaag tgaggaaata tttaaaatcg atgtctttac aagaataatt 5581 gttccagatt cttcaagcta tgtgtttttg tcatcagcgt cgtgttattc atcgcgatct 5641 taagccacag aacttgctgg tcgacaacaa tggagcaatc aagcttgccg atttcggact 5701 tgccagagct attggtatcc cgattcgcgt ttacacgcat gaagtgagtt tgcattcatt 5761 ctgtacacca atacaatgat tgtccggttt tcaggttgtc actctctggt atagagctcc 5821 agaaattctg atgggtgctc agagatactc gatgggcgta gacatgtggt cgattggatg 5881 catttttgct gaaatggcca cgaagaagcc actgtttcaa ggagattcag aaattgatga 5941 gctgttccgc atcttcagag ttctcggcac accgactgag ttggaatgga atggtgttga 6001 atcacttccg gactacaagg cgacgttccc aaaatggcgt gagaatttcc tccgtgacaa 6061 gttctacgac aagaagactg gaaagcacct tttggacgat actgcattct ctcttcttga 6121 agtaagattt ctaaaatagt actttaacaa gtaaaataat attttcaggg tttgctgatc 6181 tacgatccat cacttcgtct caacgcgaaa aaagctctcg ttcatcctta ttttgacaac 6241 atggatacgt caaagctgcc agctggaaat taccgaggag aactcgaact cttttgatgt 6301 aattcattca tcatcatttt tattatttat tctcccgtgc tttaaccacc tatcgtcttc 6361 cgtgtattgt ttaactatca ccatttcaac tttttacctg tacgatgatt atttggccct 6421 tcgatattca tgagtaatct cttttttcac accgcgataa ataaattcgc tctactttca 6481 tgctcttttt tattcaagca ctttgttcaa cgtgtaaatg agaataggga attaagaatt 6541 cattattatt atttttccat tctgaagaat gatcgctgca gtgattccaa tattcgtaat 6601 ttctctgtct aacactcgat ggggggcccg gtaccaacct aggtgagctc tggtaccctc 6661 tagtcaaggc cttaagtgag tcgtattacg tagcccgggc ctagttctag acattctcta 6721 atgaaaaaat ctttcagttg aaattgaaaa tgagttaaag ttggagtttt tattgaaaac 6781 agatttccgt gtgattagtg tttttagcga gtgtgacagg acagcgaaaa aatatagaaa 6841 caagggggga actgaaaagc ttaggaatgc attgaacatg agaaggggaa ggggaaggaa 6901 caaactagac aggaattatt ggaatttaat cacatttgga gttttttttc tattcgacag 6961 aataattatc cagaacattt ttgtattaaa tatttatgca tcatatgagt agtcggcttt 7021 gttgtgcatg acgagtttgt tatcgacgaa atagaagctg tcagaacgag tctcgtttgg 7081 attgttgatc atgtcgtcca ctgaaaaaga gattagtctt ttgaattgta ctttttagaa 7141 taatgactca ctgagttgtt gagagagttg agggaactca tagatatgtt cacagttgtt 7201 tcgtgaattc ggaatacaga atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt 7261 gaagaagtga cgttcgatca ttcggaaatg atggattggg atgtctccta ctttgaattc 7321 cacagttgct ccgaccgttt tgagtttcaa aaagtttgga gcaaatctat aacgcacgta 7381 tcttgccgac tcctgtggcg actcatcatt ctcctgatcg ttctccggtt tggcgatctc 7441 gaaaagcact tgctcagtgt ccagatcacg gatttggaac ttggtgaact cgatgttata 7501 gatgttcgca gatggggagc ataagaatcc taaatttatg ttttaaactg aaatccaaag 7561 ggagcaagat accttgagtg attcccggaa gtgctaaaac gtcgttcgga gtgatttgag 7621 ctttcttcgc aagctccgat tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg 7681 gcatctggaa atatggaaaa gttcaacaaa aagaaaagag aaaagaatga aatcggatat 7741 caagagttag ttgagcggtt tctctagttt tctgagtctc acctgcgacg ggaaggtcgc 7801 cgagccgggt ggaatcgatt gttgttgctc ggctttcata tcggtttggt tggaagcggc 7861 tgaaaacgga aagaagtgga agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta 7921 gagggtgcca aataaccagc tatattttgt ttttttttga aaacattttt aaaaagaaaa 7981 atacgataat gatatcagat ggatttccgg aaaactggta tgaaaaattt caaccttttt 8041 gagtacatgt aatcaaaata cactttgtaa attatcattt ttattgaaac tccaccattt 8101 ttctatttat aacgctaata atttgaaaaa gaaacctgtt gcgaaccgcg gggtgaatcc 8161 caaaaacgaa tgcgttttgg tggagtgatt gattcgaatc gaagaagaaa aagaagaaga 8221 cgtggaatag agagctcact cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa 8281 tgaatgaggg tcttcttctt cttcttcttc gaatgattga cagaaatggg aaaaagagga 8341 agattgagaa gggaaaaagg aaggagaaaa gaagcagaag aagacgtcag agaggagagg 8401 aacgagcgga aaagcagcgg gcgcaagtca tagaagtagc agagctgggg agaagaagac 8461 actatccaag aaaggaatga cgagagagta tgcaaagggg tatagggtgc agacagaata 8521 ggaacagaat aacagatgat gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt 8581 aacttaattt gggtcaattt gagcatgatg aattgaaatc atcccttgtt gggagttaat 8641 aaccggtttg ttatcagaaa ccctgtaata gaagggcgcc ctaactttga gccaattcat 8701 cccggtttct gtcaaatata tcaaaaagtg gtcaactgac aaattgtttt tgatattata 8761 ataaacattt tatccgttaa caattttcga atacttttta caaggacttg gataaattgg 8821 ctcaaagacg aagtgagttt gctaccatca tggaatcagg aggacatttc gacaatgtca 8881 tataatgttt gaaaaatgat tttaacaagg gtctcattcg agataagcaa tgctctgagc 8941 aagtggctcg tcgtcttcaa gaatctcttg ttgatattca tttggatcct gttttgcacg 9001 aagcatgtgc catggatatt cagagttttt gcaaaaaagt tccgccaggt caaacaatct 9061 ttttaatttt tatatgatac ttcgggtgta tttttcttct ttttcctgtt cattttcgct 9121 tttttctcac ttttttgtaa caaaatctct cacttttctg taagtcttaa caaaagttca 9181 attggctcga tcaagtaaaa tttcggccgt atgaacgttt tcaaaggttt tcattttttt 9241 ttaagttcat tcacaatatc atgaatctct cgtcctttgg tttccggtaa gtagcgaaat 9301 aatataagca tacttataaa tgaaggaatt gaatatagaa taaggaaagc ataactacca 9361 ataacggaat atagaggtaa tactgtaaat gtactgataa ccaccattac agtattcatt 9421 gaatatgcca cagattgagc aatactacga tgtttttgag gtaccagttc agaggatata 9481 aaccacgaaa ttggaccaac tccagttctg aaatttcatt attcgattaa atcaaaatat 9541 atttgaaaaa cctcacccat aagtataacc gtagatgatc aatgcaacta ggcatccgta 9601 tttcatcggg tcaatcagaa tacgtatttt tgcgaagaat acgaatgcaa ttagagctaa 9661 attattgaga aatgtgaaga gaaggagaag cttgcgtcga ccaactctgt aaacttattc 9721 atacaatttt gtcttcaaat tttgaacgtt taaaactatc ttattcaatg agttagcggg 9781 tttttatagt gagttagttc acgattatct aataaaacta tgtccaaaca tcctaactgc 9841 gaccgtattc tcactaactc gtcttaaaaa cgttgagatt attttaaacc cttcttcagt 9901 acaactacag tactgatgca caaatacaat aataataata cattcagaca tactctgaac 9961 aatgtaactt gtttttgtta tactgtggta acatttattg aaatgaaata taaccttact 10021 tttcaatcac aattccacca gtaattgttc cagaaacata agctaaaccc attgcagttg 10081 tactccattc agcaatttct gactcaacat ttgcattttc tagaaagaaa gtagaggata 10141 aaagaagagc ccataatccg acagggccc //