LOCUS Exported 5388 bp ds-DNA circular SYN 01-JUN-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5388) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 16, 2018 from SnapGene Viewer 4.1.5 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5388 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 168..289 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(296..751) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" promoter 778..882 /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: RIGHT" promoter 884..1241 /label=SV40 promoter /note="SV40 enhancer and early promoter" /note="color: #ffffff; direction: RIGHT" rep_origin 1092..1227 /label=SV40 ori /note="SV40 origin of replication" /note="color: #ffff00" CDS 1276..2070 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 2302..2349 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /note="color: #a6acb3" rep_origin 2678..3266 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" enhancer 3441..3744 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 3745..3948 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" CDS 3982..5388 /codon_start=1 /label=SypHer3s /note="color: #00ff00" /translation="MSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMYLHEAQTHQLL AQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPMADLAGEKLL MLEDGHSLRDQAMSAGFNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTP IGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLQEFVTAAGITLGMDELYNVDGGSGGT GSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPT LVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDT LVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLETLRNMVAAGSG ITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEAIRARMDG HFDKVLKQAV" ORIGIN 1 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac 61 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 121 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt 301 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 361 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 421 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 481 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 541 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 601 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 661 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 721 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 781 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 841 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 901 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 961 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 1021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 1081 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 1141 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 1201 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 1261 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 1321 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 1381 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 1441 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 1501 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 1561 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 1621 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 1681 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 1741 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 1801 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 1861 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 1921 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 1981 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 2041 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 2101 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 2161 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 2221 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 2281 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 2341 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 2401 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 2461 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 2521 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 2581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 2641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 2701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 2761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 2821 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 3001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 3061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 3121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 3181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 3241 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 3301 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 3361 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat 3421 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 3481 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 3541 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 3601 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 3661 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 3721 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 3781 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 3841 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 3901 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac 3961 cgtcagatcc gctagcgcca catgtccgga ccgctgcaca ttggtttgat tcccacagtt 4021 ggaccgtacc tgctaccgca tattatccct atgctgcacc agacctttcc aaagctggaa 4081 atgtatctgc atgaagcaca gacccaccag ttactggcgc aactggacag cggcaaactc 4141 gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt 4201 gatgagccaa tgttgctggc tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta 4261 ccgatggccg atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctctttgcgc 4321 gatcaggcaa tgtccgccgg cttcaacagc gacaacgtct atatcatggc cgacaagcag 4381 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tcgaggacgg cagcgtgcag 4441 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 4501 aaccactacc tgagcttcca gtccgtcctg agcaaagacc ccaacgagaa gcgcgatcac 4561 atggtcctgc aggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 4621 aacgtggatg gcggtagcgg tggcaccggc agcaagggcg aggagctgtt caccggggtg 4681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 4741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc 4801 aagctgcccg tgccctggcc caccctcgtg accaccctcg gctacggcct gaagtgcttc 4861 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 4921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 4981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cggcttcaag 5041 gaggacggca acatcctggg gcacaagctg gagtacaacg gcaccggttt ctgttttgaa 5101 gccggggcgg atgaagatac acacttccgc gcgaccagcc tggaaactct gcgcaacatg 5161 gtggcggcag gtagcgggat cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa 5221 cgcgatgggg ttgtttatct gccgtgcatt aagccggaac cacgccgcac tattggcctg 5281 gtttatcgtc ctggctcacc gctgcgcagc cgctatgagc agctggcaga ggccatccgc 5341 gcaagaatgg atggccattt cgataaagtt ttaaaacagg cggtttaa //