LOCUS       Exported                6586 bp ds-DNA     circular SYN 13-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SAC1-C389S∆TMD-FKBP-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Hammond Lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 16, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    592..620
                     /label=MCS
                     /note="5'"
     misc_feature    622..2183
                     /label=Sac1
     misc_feature    2185..2194
                     /label=MCS
                     /note="5'"
     misc_feature    2203..2520
                     /label=FKBP
     misc_feature    2523..2534
                     /label=3'
                     /note="3'"
     misc_feature    2542..3249
                     /label=mCherry
     misc_feature    3502..3957
                     /label=f1
                     /note="f1"
     promoter        4019..4047
                     /label=Bacterial
                     /note="Bacterial"
     promoter        4131..4360
                     /label=SV40early
                     /note="SV40early"
     misc_feature    4482..5276
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5512..5530
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5861..6504
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct catggcgacg gcggcctacg agcagctgaa gctgcatatc
      661 acacctgaaa aattttatgt ggaagcttgt gatgatggag cagatgacgt acttaccatt
      721 gaccgtgtgt ccacagaggt tacccttgca gtcaagaaag atgttcctcc ttcagctgtc
      781 acaagaccaa tatttggtat actgggcaca atccatctgg tggcaggtaa ttatcttata
      841 gtcattacca aaaagataaa agtaggtgaa tttttcagtc atgtagtctg gaaagcaaca
      901 gattttgatg tcctttctta taagaagaca atgttgcact taactgatat tcagttacaa
      961 gataataaaa ccttcctagc gatgctaaac catgtcttga atgtggatgg attttacttt
     1021 tcaacaacat atgatttgac ccatactttg cagcggctat ccaacactag tcctgaattc
     1081 caagaaatga gtctcttgga aagggcagat cagcggtttg tatggaatgg tcatcttcta
     1141 agagaacttt ctgcacagcc agaggttcat cggtttgccc ttccagtgtt acatggcttt
     1201 attaccatgc attcatgttc tattaatgga aaatactttg attggattct catctcgagg
     1261 aggagctgtt tcagagctgg tgtgcgctat tatgtaagag gaattgattc ggaaggccat
     1321 gcagctaact ttgtagaaac agaacaaatt gtgcactaca atgggagcaa agcttcgttt
     1381 gtacagactc gaggatcaat acctgttttc tggtcccaaa gaccaaacct caagtacaaa
     1441 ccactgccac agatcagcaa agtagcaaat cacatggacg gtttccaaag gcattttgat
     1501 tcccaagtaa ttatttatgg aaaacaagtt ataatcaatc tgattaacca gaagggctcg
     1561 gagaagccac ttgagcagac atttgcaaca atggtgtctt ccttgggaag tggaatgatg
     1621 agatacattg cctttgactt ccataaggaa tgtaaaaata tgagatggga tcgactaagt
     1681 attttattgg atcaggtagc agaaatgcaa gatgaattaa gttattttct agtggactct
     1741 gctggccagg tggtggcaaa ccaggaaggc gtgttccgaa gcaatagcat ggattgtcta
     1801 gatagaacca atgtgatcca gagtttgtta gctcgtcgtt cacttcaggc ccaacttcag
     1861 agactaggag ttttgcatgt gggacaaaag cttgaagaac aagatgaatt tgagaagatt
     1921 tacaaaaatg cctgggctga caacgcaaat gcttgtgcca agcaatatgc gggaactggt
     1981 gccttgaaga ctgactttac cagaactgga aagagaactc atttgggact tataatggat
     2041 ggctggaact caatgatacg atattataag aacaactttt ccgatggatt tagacaagat
     2101 tccatagact tatttcttgg aaactattca gtggatgaat tagaatctca tagtccttta
     2161 agtgttccaa gggactggaa attgtcgacg gtaccgatgg gagtgcaggt ggaaaccatc
     2221 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
     2281 gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag
     2341 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
     2401 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
     2461 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
     2521 cgggatccac cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc
     2581 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2641 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2701 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2761 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2821 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2881 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2941 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3001 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3061 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     3181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3241 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     3301 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3361 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3421 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3481 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3541 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3601 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3661 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3721 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3781 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3841 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3901 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     3961 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4021 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4081 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     4141 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4201 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4261 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4321 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4381 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4441 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     4501 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4561 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4621 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     4681 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4741 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4801 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4861 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4921 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4981 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     5041 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5101 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5161 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5221 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5281 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5341 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5401 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     5461 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     5521 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     5581 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     5641 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     5701 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     5761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6001 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6541 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
