LOCUS       Exported                6358 bp ds-DNA     circular SYN 13-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SAC1∆linker∆TMD-FKBP-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 16, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    592..620
                     /label=MCS
                     /note="5'"
     primer_bind     616..642
                     /label=Sac -TMD fwd 1
     CDS             622..1974
                     /codon_start=1
                     /label=Sac1
                     /translation="MATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVK
                     KDVPPSAVTRPIFGILGTIHLVAGNYLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTM
                     LHLTDIQLQDNKTFLAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERAD
                     QRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVR
                     YYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKV
                     ANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDF
                     HKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVI
                     QSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAG"
     primer_bind     complement(1941..1974)
                     /label=Sac link del rev 1
     misc_feature    1975..2292
                     /label=FKBP
     primer_bind     1975..1997
                     /label=Linker Insert 2
     primer_bind     complement(1990..2013)
                     /label=FKBP rev
     misc_feature    2295..2306
                     /label=3'
                     /note="3'"
     misc_feature    2314..3021
                     /label=mCherry
     misc_feature    3274..3729
                     /label=f1
                     /note="f1"
     promoter        3791..3819
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3903..4132
                     /label=SV40early
                     /note="SV40early"
     misc_feature    4254..5048
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5284..5302
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    5633..6276
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct catggcgacg gcggcctacg agcagctgaa gctgcatatc
      661 acacctgaaa aattttatgt ggaagcttgt gatgatggag cagatgacgt acttaccatt
      721 gaccgtgtgt ccacagaggt tacccttgca gtcaagaaag atgttcctcc ttcagctgtc
      781 acaagaccaa tatttggtat actgggcaca atccatctgg tggcaggtaa ttatcttata
      841 gtcattacca aaaagataaa agtaggtgaa tttttcagtc atgtagtctg gaaagcaaca
      901 gattttgatg tcctttctta taagaagaca atgttgcact taactgatat tcagttacaa
      961 gataataaaa ccttcctagc gatgctaaac catgtcttga atgtggatgg attttacttt
     1021 tcaacaacat atgatttgac ccatactttg cagcggctat ccaacactag tcctgaattc
     1081 caagaaatga gtctcttgga aagggcagat cagcggtttg tatggaatgg tcatcttcta
     1141 agagaacttt ctgcacagcc agaggttcat cggtttgccc ttccagtgtt acatggcttt
     1201 attaccatgc attcatgttc tattaatgga aaatactttg attggattct catctcgagg
     1261 aggagctgtt tcagagctgg tgtgcgctat tatgtaagag gaattgattc ggaaggccat
     1321 gcagctaact ttgtagaaac agaacaaatt gtgcactaca atgggagcaa agcttcgttt
     1381 gtacagactc gaggatcaat acctgttttc tggtcccaaa gaccaaacct caagtacaaa
     1441 ccactgccac agatcagcaa agtagcaaat cacatggacg gtttccaaag gcattttgat
     1501 tcccaagtaa ttatttatgg aaaacaagtt ataatcaatc tgattaacca gaagggctcg
     1561 gagaagccac ttgagcagac atttgcaaca atggtgtctt ccttgggaag tggaatgatg
     1621 agatacattg cctttgactt ccataaggaa tgtaaaaata tgagatggga tcgactaagt
     1681 attttattgg atcaggtagc agaaatgcaa gatgaattaa gttattttct agtggactct
     1741 gctggccagg tggtggcaaa ccaggaaggc gtgttccgaa gcaattgcat ggattgtcta
     1801 gatagaacca atgtgatcca gagtttgtta gctcgtcgtt cacttcaggc ccaacttcag
     1861 agactaggag ttttgcatgt gggacaaaag cttgaagaac aagatgaatt tgagaagatt
     1921 tacaaaaatg cctgggctga caacgcaaat gcttgtgcca agcaatatgc gggagtgcag
     1981 gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
     2041 gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac
     2101 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
     2161 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
     2221 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
     2281 ctaaaactgg aacgggatcc accggtcgcc accatggtga gcaagggcga ggaggataac
     2341 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2401 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2461 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2521 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2581 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2641 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2701 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2761 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2821 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2881 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2941 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     3001 ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac
     3061 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3121 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3181 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3241 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     3301 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3361 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3421 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3481 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3541 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3601 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3661 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3721 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3841 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     4261 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4321 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4381 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4441 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4501 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4561 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4621 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4681 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4741 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4801 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4861 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4921 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4981 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5041 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5101 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5161 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5221 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5281 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     5341 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5401 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5461 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
