LOCUS       Exported                6999 bp ds-DNA     circular SYN 13-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    mCherry-FKBP-SAC1-LX8-inflex
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 16, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     CDS             613..1320
                     /codon_start=1
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    1330
                     /label=5'
                     /note="5'"
     misc_feature    1342..1368
                     /label=AAAGAGGAA
     CDS             1384..1701
                     /codon_start=1
                     /label=FKBP
                     /note="FKBP"
                     /translation="VQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPF
                     KFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLK
                     LE"
     CDS             1708..1761
                     /codon_start=1
                     /label=GGSAx4GG
                     /translation="GGSAGGSAGGSAGGSAGG"
     misc_feature    1772..1788
                     /label=MCS
                     /note="3'"
     CDS             1789..3348
                     /codon_start=1
                     /label=SAC
                     /translation="MATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVK
                     KDVPPSAVTRPIFGILGTIHLVAGNYLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTM
                     LHLTDIQLQDNKTFLAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERAD
                     QRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVR
                     YYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKV
                     ANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDF
                     HKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVI
                     QSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTD
                     FTRTGKRTHLGLIMDGWNSMIRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPR
                     DWK"
     misc_feature    3349..3468
                     /label=Helical-Linker
     CDS             3469..3672
                     /codon_start=1
                     /label=SAC
                     /translation="FLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFF
                     IILYNGKDFVDAPRLVQKEKID"
     misc_feature    3673..3694
                     /label=MCS
                     /note="3'"
     misc_feature    3915..4370
                     /label=f1
                     /note="f1"
     promoter        4432..4460
                     /label=Bacterial
                     /note="Bacterial"
     promoter        4544..4773
                     /label=SV40early
                     /note="SV40early"
     misc_feature    4895..5689
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    5925..5943
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    6274..6917
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgaag cgcggccgcg ggagcaggag gagcagctcg agcggcgctg
     1381 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
     1441 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
     1501 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     1561 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     1621 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     1681 gtggagcttc taaaactgga aagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     1741 gctggtggta gtgctggtgg tccgcgagct caagcttcga attctgcaat ggcgacggcg
     1801 gcctacgagc agctgaagct gcatatcaca cctgaaaaat tttatgtgga agcttgtgat
     1861 gatggagcag atgacgtact taccattgac cgtgtgtcca cagaggttac ccttgcagtc
     1921 aagaaagatg ttcctccttc agctgtcaca agaccaatat ttggtatact gggcacaatc
     1981 catctggtgg caggtaatta tcttatagtc attaccaaaa agataaaagt aggtgaattt
     2041 ttcagtcatg tagtctggaa agcaacagat tttgatgtcc tttcttataa gaagacaatg
     2101 ttgcacttaa ctgatattca gttacaagat aataaaacct tcctagcgat gctaaaccat
     2161 gtcttgaatg tggatggatt ttacttttca acaacatatg atttgaccca tactttgcag
     2221 cggctatcca acactagtcc tgaattccaa gaaatgagtc tcttggaaag ggcagatcag
     2281 cggtttgtat ggaatggtca tcttctaaga gaactttctg cacagccaga ggttcatcgg
     2341 tttgcccttc cagtgttaca tggctttatt accatgcatt catgttctat taatggaaaa
     2401 tactttgatt ggattctcat ctcgaggagg agctgtttca gagctggtgt gcgctattat
     2461 gtaagaggaa ttgattcgga aggccatgca gctaactttg tagaaacaga acaaattgtg
     2521 cactacaatg ggagcaaagc ttcgtttgta cagactcgag gatcaatacc tgttttctgg
     2581 tcccaaagac caaacctcaa gtacaaacca ctgccacaga tcagcaaagt agcaaatcac
     2641 atggacggtt tccaaaggca ttttgattcc caagtaatta tttatggaaa acaagttata
     2701 atcaatctga ttaaccagaa gggctcggag aagccacttg agcagacatt tgcaacaatg
     2761 gtgtcttcct tgggaagtgg aatgatgaga tacattgcct ttgacttcca taaggaatgt
     2821 aaaaatatga gatgggatcg actaagtatt ttattggatc aggtagcaga aatgcaagat
     2881 gaattaagtt attttctagt ggactctgct ggccaggtgg tggcaaacca ggaaggcgtg
     2941 ttccgaagca attgcatgga ttgtctagat agaaccaatg tgatccagag tttgttagct
     3001 cgtcgttcac ttcaggccca acttcagaga ctaggagttt tgcatgtggg acaaaagctt
     3061 gaagaacaag atgaatttga gaagatttac aaaaatgcct gggctgacaa cgcaaatgct
     3121 tgtgccaagc aatatgcggg aactggtgcc ttgaagactg actttaccag aactggaaag
     3181 agaactcatt tgggacttat aatggatggc tggaactcaa tgatacgata ttataagaac
     3241 aacttttccg atggatttag acaagattcc atagacttat ttcttggaaa ctattcagtg
     3301 gatgaattag aatctcatag tcctttaagt gttccaaggg actggaaaga agccgctgct
     3361 agagaagctg ccgcaagaga ggccgcagcc agagaggctg ctgccaggga agcagcagct
     3421 cgggaagccg cagcacgcga ggctgcagca agagaagcag ccgcacgctt cctggctttg
     3481 cctattatca tggttgttgc cttttcaatg tgcattatct gtttgcttat ggctggtgac
     3541 acttggacag aaacactggc ctatgtgctc ttctggggag ttgcaagcat tggaacattt
     3601 tttatcattc tttacaatgg caaagatttt gtcgatgctc ccagactggt ccagaaagaa
     3661 aagatagact gaagataact gatcataatc agccatacca catttgtaga ggttttactt
     3721 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3781 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3901 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3961 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4021 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4081 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4141 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4201 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4261 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4321 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     4381 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4441 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4501 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4621 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4681 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4741 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4801 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4861 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4921 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4981 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5041 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     5101 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5161 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5221 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5281 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5341 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5401 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5461 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5521 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5581 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5641 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5701 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     5761 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5821 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     5881 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     5941 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     6001 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     6061 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     6121 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     6181 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6421 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6961 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
