LOCUS       Exported                5328 bp ds-DNA     circular SYN 13-FEB-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNES-iRFP-C1-P4MX1
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 16, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    601..645
                     /label=NES
                     /note="Value"
     misc_feature    658..1605
                     /label=iRFP
     misc_feature    1629..1645
                     /label=5'
                     /note="5'"
     misc_feature    1663..1983
                     /label=P4M
     misc_feature    1987
                     /label=3'
                     /note="3'"
     misc_feature    2244..2699
                     /label=f1
                     /note="f1"
     promoter        2761..2789
                     /label=Bacterial
                     /note="Bacterial"
     regulatory      complement(2849..3094)
                     /note="SV40_enhancer_region"
     misc_feature    3224..4018
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    4254..4272
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    4603..5246
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggctctgc agaaaaagtt ggaagagctt gagctggatg aggcaccggt cgccaccatg
      661 gcggaaggat ccgtcgccag gcagcctgac ctcttgacct gcgacgatga gccgatccat
      721 atccccggtg ccatccaacc gcatggactg ctgctcgccc tcgccgccga catgacgatc
      781 gttgccggca gcgacaacct tcccgaactc accggactgg cgatcggcgc cctgatcggc
      841 cgctctgcgg ccgatgtctt cgactcggag acgcacaacc gtctgacgat cgccttggcc
      901 gagcccgggg cggccgtcgg agcaccgatc actgtcggct tcacgatgcg aaaggacgca
      961 ggcttcatcg gctcctggca tcgccatgat cagctcatct tcctcgagct cgagcctccc
     1021 cagcgggacg tcgccgagcc gcaggcgttc ttccgccgca ccaacagcgc catccgccgc
     1081 ctgcaggccg ccgaaacctt ggaaagcgcc tgcgccgccg cggcgcaaga ggtgcggaag
     1141 attaccggct tcgatcgggt gatgatctat cgcttcgcct ccgacttcag cggcgaagtg
     1201 atcgcagagg atcggtgcgc cgaggtcgag tcaaaactag gcctgcacta tcctgcctca
     1261 accgtgccgg cgcaggcccg tcggctctat accatcaacc cggtacggat cattcccgat
     1321 atcaattatc ggccggtgcc ggtcacccca gacctcaatc cggtcaccgg gcggccgatt
     1381 gatcttagct tcgccatcct gcgcagcgtc tcgcccgtcc atctggaatt catgcgcaac
     1441 ataggcatgc acggcacgat gtcgatctcg attttgcgcg gcgagcgact gtggggattg
     1501 atcgtttgcc atcaccgaac gccgtactac gtcgatctcg atggccgcca agcctgcgag
     1561 ctagtcgccc aggttctggc ctggcagatc ggcgtgatgg aagagtccgg actcagatct
     1621 cgagctcaag cttcgaattc tgcagtcgac ggtggtagtg cttccggact cagatccacg
     1681 gcaagcacgg aaaactttaa aaatgttaaa gaaaaatatc agcaaatgcg aggtgatgct
     1741 ttaaaaacag aaatcctggc tgatttcaag gataaactgg ctgaagctac cgatgaacag
     1801 agccttaagc aaattgttgc tgaattgaaa agtaaagatg aatatagaat tttggctaag
     1861 gggcaagggt taacaaccca gcttttaggg ttaaagacaa gttcagtgtc ttcatttgag
     1921 aaaatggttg aggaaacaag agaaagtatt aagtctcaag aaagacaaac cattaagata
     1981 aaataaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     2041 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2101 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2221 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     2281 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2341 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2401 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2461 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2521 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2581 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     2641 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     2701 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2821 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2941 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3001 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3181 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3241 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3301 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3361 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3421 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3481 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3541 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3601 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3661 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3721 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     3781 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3841 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3901 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3961 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4021 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4081 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4141 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     4201 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     4261 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4321 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4381 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4441 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     4501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4741 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5281 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
