LOCUS       FUW_dCas9_Tet1CD       16359 bp ds-DNA     circular     26-OCT-2016
DEFINITION  cloning vector.
KEYWORDS    CAGGS promoter; amp resistant; HIV LTR; WRE; zeocin; human
            ubiquitin promoter enhancer; human ubiquitin intron; lentivirus.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        212..816
                     /vntifkey="29"
                     /label=CMV\promoter/enhancer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10331..11032
                     /label=tagBFP
                     /ApEinfo_fwdcolor=#5555ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             835..1509
                     /vntifkey="19"
                     /label=5'\HIVR-U5
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8009..8029
                     /label=NLS (SV 40)
                     /ApEinfo_fwdcolor=#80ffff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    1533..2390
                     /vntifkey="20"
                     /label=HIV\rev\response\element
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8054..8056
                     /label=linker of D
                     /ApEinfo_fwdcolor=#004080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    2416..2593
                     /vntifkey="20"
                     /label=HIV\flap/nuclear\import\signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(3890..5940,5942..7993)
                     /label=dCas
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6879..6894
                     /label=Seqencing primer_SL-61
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2622..3144
                     /vntifkey="29"
                     /label=human\ubiquitin\promoter/enhancer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          3145..3833
                     /vntifkey="15"
                     /label=human\ubiquitin\intron
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8849..8851
                     /label=D1674
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    11051..11639
                     /vntifkey="21"
                     /label=WRE
                     /ApEinfo_fwdcolor=#400080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5142..5156
                     /label=Sequencing primer_SL-58
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8084..10237
                     /label=Tet1CD (with 3 silent mutations)
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9987..10001
                     /label=Sequencing Primer_SL-49
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             11655..12350
                     /vntifkey="19"
                     /label=HIV\delU3RdelU5
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_marker     13530..13903
                     /vntifkey="22"
                     /label=ZEO
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7908..7923
                     /label=Sequencing Primer_SL-47
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_marker     15369..16227
                     /vntifkey="22"
                     /label=AMP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7994..8008
                     /label=GGGGS linker
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5775..5789
                     /label=Sequencing primer_SL-59
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8033..8053
                     /label=NLS
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8387..8401
                     /label=Sequencing Primer_SL-48
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8057..8077
                     /label=NLS(1)
                     /ApEinfo_label=NLS
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9987..10001
                     /label=Sequencing primer_SL-49
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3686..3700
                     /label=SL-53 sequencing primer
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    11126..11140
                     /label=SL-54 sequencing primer rev
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9526..9543
                     /label=Sequencing primer_SL-351, For
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7407..7422
                     /label=Sequencing primer_SL-62
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3869..3874
                     /label=HpaI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6254..6268
                     /label=Sequencing primer_SL-60
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8843..8845
                     /label=H1672
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3917..3919
                     /label=D10A mutation
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10244..10273
                     /label=HA tag
                     /ApEinfo_fwdcolor=#80ffff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6407..6409
                     /label=H840A mutation
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3881..3888
                     /label=AscI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10238..10243
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8078..8083
                     /label=BamHI site
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3856..3861
                     /label=XbaI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10274..10330
                     /label=P2A
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4045..4062
                     /label=SL-351, For
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8387..8401
                     /label=Sequencing primer_SL-48
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9011..9026
                     /label=Sequencing primer_SL-63
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4518..4532
                     /label=Sequencing primer_SL-57
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2474..2492
                     /label=SL-614
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3923..3940
                     /label=SL-615 rev
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgacTCTAG AGGATCCaGT TAACctcgag GGCGCGCCCA TGGACAAGAA
     3901 GTATTCTATC GGACTGGCCA TCGGGACTAA TAGCGTCGGG TGGGCCGTGA TCACTGACGA
     3961 GTACAAGGTG CCCTCTAAGA AGTTCAAGGT GCTCGGGAAC ACCGACCGGC ATTCCATCAA
     4021 GAAAAATCTG ATCGGAGCTC TCCTCTTTGA TTCAGGGGAG ACCGCTGAAG CAACCCGCCT
     4081 CAAGCGGACT GCTAGACGGC GGTACACCAG GAGGAAGAAC CGGATTTGTT ACCTTCAAGA
     4141 GATATTCTCC AACGAAATGG CAAAGGTCGA CGACAGCTTC TTCCATAGGC TGGAAGAATC
     4201 ATTCCTCGTG GAAGAGGATA AGAAGCATGA ACGGCATCCC ATCTTCGGTA ATATCGTCGA
     4261 CGAGGTGGCC TATCACGAGA AATACCCAAC CATCTACCAT CTTCGCAAAA AGCTGGTGGA
     4321 CTCAACCGAC AAGGCAGACC TCCGGCTTAT CTACCTGGCC CTGGCCCACA TGATCAAGTT
     4381 CAGAGGCCAC TTCCTGATCG AGGGCGACCT CAATCCTGAC AATAGCGATG TGGATAAACT
     4441 GTTCATCCAG CTGGTGCAGA CTTACAACCA GCTCTTTGAA GAGAACCCCA TCAATGCAAG
     4501 CGGAGTCGAT GCCAAGGCCA TTCTGTCAGC CCGGCTGTCA AAGAGCCGCA GACTTGAGAA
     4561 TCTTATCGCT CAGCTGCCGG GTGAAAAGAA AAATGGACTG TTCGGGAACC TGATTGCTCT
     4621 TTCACTTGGG CTGACTCCCA ATTTCAAGTC TAATTTCGAC CTGGCAGAGG ATGCCAAGCT
     4681 GCAACTGTCC AAGGACACCT ATGATGACGA TCTCGACAAC CTCCTGGCCC AGATCGGTGA
     4741 CCAATACGCC GACCTTTTCC TTGCTGCTAA GAATCTTTCT GACGCCATCC TGCTGTCTGA
     4801 CATTCTCCGC GTGAACACTG AAATCACCAA GGCCCCTCTT TCAGCTTCAA TGATTAAGCG
     4861 GTATGATGAG CACCACCAGG ACCTGACCCT GCTTAAGGCA CTCGTCCGGC AGCAGCTTCC
     4921 GGAGAAGTAC AAGGAAATCT TCTTTGACCA GTCAAAGAAT GGATACGCCG GCTACATCGA
     4981 CGGAGGTGCC TCCCAAGAGG AATTTTATAA GTTTATCAAA CCTATCCTTG AGAAGATGGA
     5041 CGGCACCGAA GAGCTCCTCG TGAAACTGAA TCGGGAGGAT CTGCTGCGGA AGCAGCGCAC
     5101 TTTCGACAAT GGGAGCATTC CCCACCAGAT CCATCTTGGG GAGCTTCACG CCATCCTTCG
     5161 GCGCCAAGAG GACTTCTACC CCTTTCTTAA GGACAACAGG GAGAAGATTG AGAAAATTCT
     5221 CACTTTCCGC ATCCCCTACT ACGTGGGACC CCTCGCCAGA GGAAATAGCC GGTTTGCTTG
     5281 GATGACCAGA AAGTCAGAAG AAACTATCAC TCCCTGGAAC TTCGAAGAGG TGGTGGACAA
     5341 GGGAGCCAGC GCTCAGTCAT TCATCGAACG GATGACTAAC TTCGATAAGA ACCTCCCCAA
     5401 TGAGAAGGTC CTGCCGAAAC ATTCCCTGCT CTACGAGTAC TTTACCGTGT ACAACGAGCT
     5461 GACCAAGGTG AAATATGTCA CCGAAGGGAT GAGGAAGCCC GCATTCCTGT CAGGCGAACA
     5521 AAAGAAGGCA ATTGTGGACC TTCTGTTCAA GACCAATAGA AAGGTGACCG TGAAGCAGCT
     5581 GAAGGAGGAC TATTTCAAGA AAATTGAATG CTTCGACTCT GTGGAGATTA GCGGGGTCGA
     5641 AGATCGGTTC AACGCAAGCC TGGGTACCTA CCATGATCTG CTTAAGATCA TCAAGGACAA
     5701 GGATTTTCTG GACAATGAGG AGAACGAGGA CATCCTTGAG GACATTGTCC TGACTCTCAC
     5761 TCTGTTCGAG GACCGGGAAA TGATCGAGGA GAGGCTTAAG ACCTACGCCC ATCTGTTCGA
     5821 CGATAAAGTG ATGAAGCAAC TTAAACGGAG AAGATATACC GGATGGGGAC GCCTTAGCCG
     5881 CAAACTCATC AACGGAATCC GGGACAAACA GAGCGGAAAG ACCATTCTTG ATTTCCTTAA
     5941 AAGCGACGGA TTCGCTAATC GCAACTTCAT GCAACTTATC CATGATGATT CCCTGACCTT
     6001 TAAGGAGGAC ATCCAGAAGG CCCAAGTGTC TGGACAAGGT GACTCACTGC ACGAGCATAT
     6061 CGCAAATCTG GCTGGTTCAC CCGCTATTAA GAAGGGTATT CTCCAGACCG TGAAAGTCGT
     6121 GGACGAGCTG GTCAAGGTGA TGGGTCGCCA TAAACCAGAG AACATTGTCA TCGAGATGGC
     6181 CAGGGAAAAC CAGACTACCC AGAAGGGACA GAAGAACAGC AGGGAGCGGA TGAAAAGAAT
     6241 TGAGGAAGGG ATTAAGGAGC TCGGGTCACA GATCCTTAAA GAGCACCCGG TGGAAAACAC
     6301 CCAGCTTCAG AATGAGAAGC TCTATCTGTA CTACCTTCAA AATGGACGCG ATATGTATGT
     6361 GGACCAAGAG CTTGATATCA ACAGGCTCTC AGACTACGAC GTGGACGCCA TCGTCCCTCA
     6421 GAGCTTCCTC AAAGACGACT CAATTGACAA TAAGGTGCTG ACTCGCTCAG ACAAGAACCG
     6481 GGGAAAGTCA GATAACGTGC CCTCAGAGGA AGTCGTGAAA AAGATGAAGA ACTATTGGCG
     6541 CCAGCTTCTG AACGCAAAGC TGATCACTCA GCGGAAGTTC GACAATCTCA CTAAGGCTGA
     6601 GAGGGGCGGA CTGAGCGAAC TGGACAAAGC AGGATTCATT AAACGGCAAC TTGTGGAGAC
     6661 TCGGCAGATT ACTAAACATG TCGCCCAAAT CCTTGACTCA CGCATGAATA CCAAGTACGA
     6721 CGAAAACGAC AAACTTATCC GCGAGGTGAA GGTGATTACC CTGAAGTCCA AGCTGGTCAG
     6781 CGATTTCAGA AAGGACTTTC AATTCTACAA AGTGCGGGAG ATCAATAACT ATCATCATGC
     6841 TCATGACGCA TATCTGAATG CCGTGGTGGG AACCGCCCTG ATCAAGAAGT ACCCAAAGCT
     6901 GGAAAGCGAG TTCGTGTACG GAGACTACAA GGTCTACGAC GTGCGCAAGA TGATTGCCAA
     6961 ATCTGAGCAG GAGATCGGAA AGGCCACCGC AAAGTACTTC TTCTACAGCA ACATCATGAA
     7021 TTTCTTCAAG ACCGAAATCA CCCTTGCAAA CGGTGAGATC CGGAAGAGGC CGCTCATCGA
     7081 GACTAATGGG GAGACTGGCG AAATCGTGTG GGACAAGGGC AGAGATTTCG CTACCGTGCG
     7141 CAAAGTGCTT TCTATGCCTC AAGTGAACAT CGTGAAGAAA ACCGAGGTGC AAACCGGAGG
     7201 CTTTTCTAAG GAATCAATCC TCCCCAAGCG CAACTCCGAC AAGCTCATTG CAAGGAAGAA
     7261 GGATTGGGAC CCTAAGAAGT ACGGCGGATT CGATTCACCA ACTGTGGCTT ATTCTGTCCT
     7321 GGTCGTGGCT AAGGTGGAAA AAGGAAAGTC TAAGAAGCTC AAGAGCGTGA AGGAACTGCT
     7381 GGGTATCACC ATTATGGAGC GCAGCTCCTT CGAGAAGAAC CCAATTGACT TTCTCGAAGC
     7441 CAAAGGTTAC AAGGAAGTCA AGAAGGACCT TATCATCAAG CTCCCAAAGT ATAGCCTGTT
     7501 CGAACTGGAG AATGGGCGGA AGCGGATGCT CGCCTCCGCT GGCGAACTTC AGAAGGGTAA
     7561 TGAGCTGGCT CTCCCCTCCA AGTACGTGAA TTTCCTCTAC CTTGCAAGCC ATTACGAGAA
     7621 GCTGAAGGGG AGCCCCGAGG ACAACGAGCA AAAGCAACTG TTTGTGGAGC AGCATAAGCA
     7681 TTATCTGGAC GAGATCATTG AGCAGATTTC CGAGTTTTCT AAACGCGTCA TTCTCGCTGA
     7741 TGCCAACCTC GATAAAGTCC TTAGCGCATA CAATAAGCAC AGAGACAAAC CAATTCGGGA
     7801 GCAGGCTGAG AATATCATCC ACCTGTTCAC CCTCACCAAT CTTGGTGCCC CTGCCGCATT
     7861 CAAGTACTTC GACACCACCA TCGACCGGAA ACGCTATACC TCCACCAAAG AAGTGCTGGA
     7921 CGCCACCCTC ATCCACCAGA GCATCACCGG ACTTTACGAA ACTCGGATTG ACCTCTCACA
     7981 GCTCGGAGGG GATGGTGGCG GAGGCTCGCC AAAAAAGAAG AGAAAGGTAG ACCCAAAGAA
     8041 AAAACGAAAA GTAGATCCGA AAAAGAAGAG GAAGGTGGGA TCCCTGCCCA CCTGCAGCTG
     8101 TCTTGATCGA GTTATACAAA AAGACAAAGG CCCATATTAT ACACACCTTG GGGCAGGACC
     8161 AAGTGTTGCT GCTGTCAGGG AAATCATGGA GAATAGGTAT GGTCAAAAAG GAAACGCAAT
     8221 AAGGATAGAA ATAGTAGTGT ACACCGGTAA AGAAGGGAAA AGCTCTCATG GGTGTCCAAT
     8281 TGCTAAGTGG GTTTTAAGAA GAAGCAGTGA TGAAGAAAAA GTTCTTTGTT TGGTCCGGCA
     8341 GCGTACAGGC CACCACTGTC CAACTGCTGT GATGGTGGTG CTCATCATGG TGTGGGATGG
     8401 CATCCCTCTT CCAATGGCCG ACCGGCTATA CACAGAGCTC ACAGAGAATC TAAAGTCATA
     8461 CAATGGGCAC CCTACCGACA GAAGATGCAC CCTCAATGAA AATCGTACCT GTACATGTCA
     8521 AGGAATTGAT CCAGAGACTT GTGGAGCTTC ATTCTCTTTT GGCTGTTCAT GGAGTATGTA
     8581 CTTTAATGGC TGTAAGTTTG GTAGAAGCCC AAGCCCCAGA AGATTTAGAA TTGATCCAAG
     8641 CTCTCCCTTA CATGAAAAAA ACCTTGAAGA TAACTTACAG AGTTTGGCTA CACGATTAGC
     8701 TCCAATTTAT AAGCAGTATG CTCCAGTAGC TTACCAAAAT CAGGTGGAAT ATGAAAATGT
     8761 TGCCCGAGAA TGTCGGCTTG GCAGCAAGGA AGGTCGACCC TTCTCTGGGG TCACTGCTTG
     8821 CCTGGACTTC TGTGCTCATC CCCACAGGGA CATTCACAAC ATGAATAATG GAAGCACTGT
     8881 GGTTTGTACC TTAACTCGAG AAGATAACCG CTCTTTGGGT GTTATTCCTC AAGATGAGCA
     8941 GCTCCATGTG CTACCTCTTT ATAAGCTTTC AGACACAGAT GAGTTTGGCT CCAAGGAAGG
     9001 AATGGAAGCC AAGATCAAAT CTGGGGCCAT CGAGGTCCTG GCACCCCGCC GCAAAAAAAG
     9061 AACGTGTTTC ACTCAGCCTG TTCCCCGTTC TGGAAAGAAG AGGGCTGCGA TGATGACAGA
     9121 GGTTCTTGCA CATAAGATAA GGGCAGTGGA AAAGAAACCT ATTCCCCGAA TCAAGCGGAA
     9181 GAATAACTCA ACAACAACAA ACAACAGTAA GCCTTCGTCA CTGCCAACCT TAGGGAGTAA
     9241 CACTGAGACC GTGCAACCTG AAGTAAAAAG TGAAACCGAA CCCCATTTTA TCTTAAAAAG
     9301 TTCAGACAAC ACTAAAACTT ATTCGCTGAT GCCATCCGCT CCTCACCCAG TGAAAGAGGC
     9361 ATCTCCAGGC TTCTCCTGGT CCCCGAAGAC TGCTTCAGCC ACACCAGCTC CACTGAAGAA
     9421 TGACGCAACA GCCTCATGCG GGTTTTCAGA AAGAAGCAGC ACTCCCCACT GTACGATGCC
     9481 TTCGGGAAGA CTCAGTGGTG CCAATGCTGC AGCTGCTGAT GGCCCTGGCA TTTCACAGCT
     9541 TGGCGAAGTG GCTCCTCTCC CCACCCTGTC TGCTCCTGTG ATGGAGCCCC TCATTAATTC
     9601 TGAGCCTTCC ACTGGTGTGA CTGAGCCGCT AACGCCTCAT CAGCCAAACC ACCAGCCCTC
     9661 CTTCCTCACC TCTCCTCAAG ACCTTGCCTC TTCTCCAATG GAAGAAGATG AGCAGCATTC
     9721 TGAAGCAGAT GAGCCTCCAT CAGACGAACC CCTATCTGAT GACCCCCTGT CACCTGCTGA
     9781 GGAGAAATTG CCCCACATTG ATGAGTATTG GTCAGACAGT GAGCACATCT TTTTGGATGC
     9841 AAATATTGGT GGGGTGGCCA TCGCACCTGC TCACGGCTCG GTTTTGATTG AGTGTGCCCG
     9901 GCGAGAGCTG CACGCTACCA CTCCTGTTGA GCACCCCAAC CGTAATCATC CAACCCGCCT
     9961 CTCCCTTGTC TTTTACCAGC ACAAAAACCT AAATAAGCCC CAACATGGTT TTGAACTAAA
    10021 CAAGATTAAG TTTGAGGCTA AAGAAGCTAA GAATAAGAAA ATGAAGGCCT CAGAGCAAAA
    10081 AGACCAGGCA GCTAATGAAG GTCCAGAACA GTCCTCTGAA GTAAATGAAT TGAACCAAAT
    10141 TCCTTCTCAT AAAGCATTAA CATTAACCCA TGACAATGTT GTCACCGTGT CCCCTTATGC
    10201 TCTCACACAC GTTGCGGGGC CCTATAACCA TTGGGTCGAA TTCGCATACC CCTACGACGT
    10261 CCCCGACTAC GCCGCCACGA ACTTCTCTCT GTTAAAGCAA GCAGGAGATG TTGAAGAAAA
    10321 CCCCGGGCCT ATGAGCGAGC TGATTAAGGA GAACATGCAC ATGAAGCTGT ACATGGAGGG
    10381 CACCGTGGAC AACCATCACT TCAAGTGCAC ATCCGAGGGC GAAGGCAAGC CCTACGAGGG
    10441 CACCCAGACC ATGAGAATCA AGGTGGTCGA GGGCGGCCCT CTCCCCTTCG CCTTCGACAT
    10501 CCTGGCTACT AGCTTCCTCT ACGGCAGCAA GACCTTCATC AACCACACCC AGGGCATCCC
    10561 CGACTTCTTC AAGCAGTCCT TCCCTGAGGG CTTCACATGG GAGAGAGTCA CCACATACGA
    10621 AGACGGGGGC GTGCTGACCG CTACCCAGGA CACCAGCCTC CAGGACGGCT GCCTCATCTA
    10681 CAACGTCAAG ATCAGAGGGG TGAACTTCAC ATCCAACGGC CCTGTGATGC AGAAGAAAAC
    10741 ACTCGGCTGG GAGGCCTTCA CCGAGACGCT GTACCCCGCT GACGGCGGCC TGGAAGGCAG
    10801 AAACGACATG GCCCTGAAGC TCGTGGGCGG GAGCCATCTG ATCGCAAACA TCAAGACCAC
    10861 ATATAGATCC AAGAAACCCG CTAAGAACCT CAAGATGCCT GGCGTCTACT ATGTGGACTA
    10921 CAGACTGGAA AGAATCAAGG AGGCCAACAA CGAGACCTAC GTCGAGCAGC ACGAGGTGGC
    10981 AGTGGCCAGA TACTGCGACC TCCCTAGCAA ACTGGGGCAC AAGCTTAATT GAgatatcaa
    11041 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    11101 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    11161 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    11221 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    11281 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    11341 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    11401 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
    11461 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    11521 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
    11581 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
    11641 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
    11701 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
    11761 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    11821 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
    11881 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
    11941 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    12001 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
    12061 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
    12121 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
    12181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    12241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    12301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
    12361 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    12421 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    12481 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    12541 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    12601 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
    12661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    12721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    12781 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    12841 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    12901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    12961 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    13021 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
    13081 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    13141 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    13201 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    13261 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    13321 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    13381 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    13441 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
    13501 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
    13561 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
    13621 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
    13681 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
    13741 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
    13801 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
    13861 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
    13921 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    13981 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    14041 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    14101 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    14161 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    14221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    14281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    14341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    14401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    14461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    14521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    14581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    14641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    14701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    14761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    14821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    14881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    14941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    15001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    15061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    15121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    15181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    15241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    15301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    15361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    15421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    15481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    15541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    15601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    15661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    15721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    15781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    15841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    15901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    15961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    16021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    16081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    16141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    16201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    16261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    16321 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac  
//
