LOCUS       pET28a-MH6-EsCas13d     8252 bp ds-DNA     circular     05-MAR-2018
DEFINITION  .
ACCESSION   DS499551                                                           
VERSION     DS499551                                                           
FEATURES             Location/Qualifiers
     pABR_INS        8031..8123
                     /label="XhoI_+_J23119_+_2x_BsaI_+_SacI_+_XhoI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      8124..8252
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6039..6056
                     /label="HEPN domain"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     pABR_INS        5157..8018
                     /label="Effector_A"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        4984..5002
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     pABR_INS        8019..8030
                     /label="2x_STOP_+_EcoRI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5002..5029
                     /label="lacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     pABR_INS        5115..5156
                     /label="mH6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    8180..8227
                     /label="Hairpin"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2499..2521
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2515..2706)
                     /label="ROP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            complement(560..1375)
                     /label="KanR2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3515..4606)
                     /label="lacI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            complement(4689..4952)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(3181..3456)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1481..2100
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     pABR_INS        5030..5114
                     /label="XbaI_+_Lac_+_XbaI/EcoRI_+_RBS_+_3'_UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7701..7718
                     /label="HEPN domain"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagatttac
     5041 actttatgct tccggctcgt atgtttctag agaattcaaa taattttgtt taactttaag
     5101 aaggagattt aaatatgaaa atcgaagaag gtaaaggtca ccatcaccat caccacatgg
     5161 gcaaaaaaat ccatgcacgt gatctgcgtg aacagcgtaa aaccgatcgt accgaaaaat
     5221 ttgcagacca gaacaaaaaa cgtgaagcag aacgtgcagt tccgaaaaaa gatgcagcag
     5281 ttagcgttaa aagcgttagc agcgtgagca gcaaaaaaga taatgttacc aaaagcatgg
     5341 caaaagcagc cggtgtgaaa agcgtttttg cagttggtaa taccgtttac atgaccagct
     5401 ttggtcgtgg taatgatgca gttctggaac agaaaattgt tgataccagc catgaaccgc
     5461 tgaatattga tgatccggca tatcagctga atgtggttac catgaatggt tatagcgtta
     5521 ccggtcatcg tggtgaaacc gttagcgcag ttaccgataa tccgctgcgt cgttttaatg
     5581 gtcgtaaaaa agacgaaccg gaacagagcg ttccgaccga tatgctgtgt ctgaaaccga
     5641 cactggaaaa aaagtttttt ggcaaagagt tcgacgacaa cattcatatc cagctgattt
     5701 ataacatcct ggacatcgaa aaaatcctgg cagtttatag caccaatgca atttatgccc
     5761 tgaataacat gagcgcagat gaaaacattg agaacagcga cttttttatg aaacgcacca
     5821 ccgatgaaac cttcgatgat ttcgaaaaaa agaaagaaag caccaacagc cgtgaaaaag
     5881 cagattttga tgccttcgaa aaattcatcg gtaattatcg cctggcctat tttgcagatg
     5941 ccttttatgt caacaaaaag aacccgaaag gcaaagccaa aaatgtgctg cgtgaagata
     6001 aagaactgta tagcgttctg accctgattg gtaaactgcg tcattggtgt gttcatagcg
     6061 aagaaggtcg cgcagaattt tggctgtata aactggatga actgaaggac gatttcaaaa
     6121 acgttctgga tgtggtttat aatcgtccgg tggaagaaat taacaaccgc tttatcgaaa
     6181 acaacaaagt gaacattcag attctgggca gcgtgtataa aaacaccgat attgcagaac
     6241 tggtgcgcag ctattatgaa tttctgatca ccaaaaaata caaaaacatg ggctttagca
     6301 tcaaaaaact gcgtgaaagc atgctggaag gtaaaggtta tgccgataaa gaatatgata
     6361 gcgtgcgcaa caaactgtat cagatgaccg attttattct gtacaccggc tatatcaacg
     6421 aagatagcga tcgtgcagat gatctggtta ataccctgcg tagcagcctg aaagaagatg
     6481 ataaaaccac cgtgtattgc aaagaggcag attacctttg gaaaaagtat cgcgaaagca
     6541 ttcgtgaagt tgccgatgca ctggatggtg acaatattaa gaaactgagc aaaagcaaca
     6601 tcgagatcca agaggataaa ctgcgcaaat gctttattag ctatgcagat agcgttagcg
     6661 aatttaccaa actgatttat ctgctgaccc gttttctgag cggtaaagaa atcaacgatc
     6721 tggtgaccac actgatcaac aaatttgata acattcgcag ctttctggaa atcatggatg
     6781 aattaggtct ggatcgtacc tttaccgcag aatatagctt ttttgaaggc agcaccaaat
     6841 atctggccga actggtggaa ctgaatagct ttgttaaatc ctgcagcttc gatattaatg
     6901 ccaaacgtac catgtatcgt gatgccctgg atattctggg tattgaatca gataaaaccg
     6961 aagaggatat cgagaaaatg atcgacaata tcctgcagat tgatgccaac ggtgataaaa
     7021 agctgaaaaa aaacaatggc ctgcgcaact ttattgccag caatgttatt gatagcaacc
     7081 gcttcaaata cctggtgcgt tatggtaacc cgaagaaaat tcgtgaaacc gcaaaatgta
     7141 aaccggcagt tcgttttgtg ctgaatgaaa ttccggatgc acagatcgaa cgttattatg
     7201 aagcatgttg tccgaaaaat accgcactgt gtagcgcaaa taaacgtcgt gaaaaactgg
     7261 cagatatgat cgccgaaatc aaattcgaaa acttttccga tgcaggcaac tatcagaaag
     7321 caaatgtgac cagccgtacc agcgaagcag aaattaaacg taaaaaccag gccattattc
     7381 gcctgtatct gaccgttatg tatatcatgc tgaaaaacct ggtgaatgtg aatgcccgtt
     7441 atgtgattgc atttcattgt gttgaacgcg acacaaaact gtatgccgaa agcggtctgg
     7501 aagtgggtaa tattgaaaag aataaaacca acctgaccat ggccgttatg ggtgttaaac
     7561 tggaaaatgg cattatcaaa accgagttcg ataagagctt tgcagaaaat gcagcaaatc
     7621 gttatctgcg taatgcgcgt tggtataagc tgattctgga taatctgaag aaaagcgaac
     7681 gtgccgttgt gaatgaattt cgcaataccg tgtgtcatct gaatgccatt cgcaacatta
     7741 acatcaacat taaagagatc aaagaggtcg agaactattt cgccctgtat cattatctga
     7801 tccagaaaca tctggaaaac cgcttcgcag acaaaaaagt tgaacgtgat accggtgatt
     7861 tcatcagcaa actggaagaa cataaaacct actgcaaaga cttcgtgaaa gcctattgta
     7921 ccccgtttgg ttataatctg gttcgctata aaaacctgac gatcgatggt ctgttcgaca
     7981 aaaactatcc gggtaaagat gatagcgacg agcagaaata ataagaattc ctcgagttga
     8041 cagctagctc agtcctaggt ataatgctag cagagaccat ggccaccggt ctctgctaaa
     8101 gatatgagga gctcaaactc gagctgctaa caaagcccga aaggaagctg agttggctgc
     8161 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     8221 ttttttgctg aaaggaggaa ctatatccgg at
//