LOCUS pET28a-MH6-RspCas13d 8153 bp ds-DNA circular 05-MAR-2018 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(5682..5713) /label="afT7termrv" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Library Acceptor 5497..5548 /label="BsaI-filler-BsaI" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Promoter 2508..2526 /label="pET28 T7 promoter" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Affinity Tag 2639..2680 /label="mH6 tag" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Operon 2526..2553 /label="LacO" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Promoter 2560..2589 /label="Lac promoter" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 RBS 2602..2638 /label="pET RBS + 3' UTR" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2681..5437 /label="LARF effector" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Promoter 5462..5496 /label="J23119 promoter" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 ORIGIN 1 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 61 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag 121 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg 181 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc 241 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc 301 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa 361 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc 421 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg 481 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg 541 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt 601 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg 661 gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg 721 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg 781 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg 841 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag 901 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact 961 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt 1021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 1081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt 1141 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga 1201 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt 1261 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat 1321 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg 1381 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg 1441 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt 1501 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg 1561 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg 1621 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag 1681 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag 1741 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt 1801 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc 1861 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt 1921 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat 1981 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct 2041 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc 2101 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca 2161 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct 2221 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg 2281 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg 2341 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 2401 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 2461 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac 2521 tataggggaa ttgtgagcgg ataacaattc ccctctagaT TTACACTTTA TGCTTCCGGC 2581 TCGTATGTTt ctagagaatt caaataattt tgtttaactt taagaaggag ATTTAAATat 2641 gAAAATCGAA GAAGGTAAAG GTCACCATCA CCATCACCAC ATGGCCAAAA AGAACAAAAT 2701 GAAACCGCGT GAACTGCGTG AAGCACAGAA AAAAGCACGT CAGCTGAAAG CAGCAGAAAT 2761 TAACAATAAT GCAGCACCGG CAATTGCAGC AATGCCTGCA GCAGAAGTTA TTGCACCGGT 2821 TGCAGAAAAA AAGAAAAGCA GCGTTAAAGC AGCCGGTATG AAAAGCATTC TGGTGAGCAA 2881 AAACAAAATG TATATCACCA GCTTTGGCAA AGGTAATAGC GCAGTTCTGG AATATGAAGT 2941 GGATAACAAC GATTACAATC AGACCCAGCT GAGCAGCAAA GGTAGCAGCA ATATTGAGCT 3001 GCGTGGTGTT AATGAAGTGA ACATTACCTT TAGCAGCAAA CACGGTTTTG AAAGCGGTGT 3061 TGAAATCAAT ACCAGCAATC CGACACATCG TAGCGGTGAA AGCAGTCCGG TTCGTGGTGA 3121 TATGCTGGGC CTGAAAAGCG AACTGGAAAA ACGCTTTTTT GGCAAAACCT TCGATGACAA 3181 CATTCATATC CAGCTGATCT ATAACATCCT GGACATCGAA AAAATTCTGG CCGTTTATGT 3241 GACCAACATT GTGTATGCAC TGAATAACAT GCTGAGCATT AAAGATAGCG AGAGCTATGA 3301 TGATTTCATG GGTTATCTGA GCGCACGCAA TACCTATGAA GTTTTTACCC ATCCGGATAA 3361 AAGCAACCTG AGCGATAAAG CAAAAGGCAA CATCAAAAAA TCCTTTAGCA CCTTCAACGA 3421 CCTGCTGAAA ACCAAACGTC TGGGTTATTT TGGTCTGGAA GAACCGAAAA CAAAAGATAC 3481 CCGTGTTAGC CAGGCATACA AAAAACGTGT TTATCACATG CTGGCAATTG TGGGTCAGAT 3541 TCGTCAGAGC GTGTTTCATG ATAAAAGTAG CAAACTGGAT GAGGACCTGT ATAGCTTTAT 3601 CGATATCATC GATTCCGAAT ATCGTGAAAC CCTGGATTAT CTGGTTGATG AACGTTTTGA 3661 CAGCATCAAC AAAGGTTTTA TCCAGGGCAA CAAAGTGAAT ATTAGCCTGC TGATCGATAT 3721 GATGAAAGGC TATGAAGCCG ATGATATTAT CCGCCTGTAT TATGATTTTA TCGTGCTGAA 3781 AAGCCAGAAG AATCTGGGCT TTAGTATCAA AAAACTGCGC GAGAAAATGC TGGATGAATA 3841 TGGCTTTCGC TTCAAAGATA AACAGTATGA TAGCGTGCGT AGCAAGATGT ATAAACTGAT 3901 GGATTTTCTG CTGTTCTGCA ACTATTATCG CAATGATGTT GTTGCCGGTG AAGCACTGGT 3961 TCGTAAACTG CGTTTTAGCA TGACCGATGA TGAAAAAGAA GGTATTTACG CAGACGAAGC 4021 AAGCAAACTG TGGGGTAAAT TTCGTAACGA TTTTGAGAAC ATTGCCGACC ATATGAACGG 4081 TGATGTTATT AAAGAACTGG GCAAAGCCGA TATGGATTTC GATGAAAAAA TACTGGACAG 4141 CGAGAAAAAA AACGCAAGCG ATCTGCTGTA TTTCAGCAAG ATGATTTATA TGCTGACCTA 4201 TTTCCTGGAT GGCAAAGAAA TTAATGATCT GCTGACCACG CTGATTAGCA AATTTGATAA 4261 CATCAAAGAA TTTCTGAAAA TCATGAAAAG CTCAGCCGTT GATGTTGAAT GTGAACTGAC 4321 CGCAGGTTAT AAACTGTTTA ATGATAGCCA GCGCATTACC AACGAACTGT TCATTGTTAA 4381 AAACATTGCG AGCATGCGTA AACCGGCAAG CAGCGCAAAA CTGACCATGT TTCGTGATGC 4441 ACTGACCATT CTGGGTATTG ATGATAACAT TACCGATGAT CGCATTAGCG AAATCCTGAA 4501 GCTGAAAGAA AAAGGTAAAG GTATTCATGG CCTGCGCAAC TTTATTACCA ATAACGTGAT 4561 TGAAAGCTCC CGCTTTGTGT ACCTGATCAA ATATGCAAAT GCCCAGAAAA TTCGCAAAGT 4621 GGCGAAAAAT GAAAAGGTGG TGATGTTTGT GTTAGGTGGT ATTCCGGATA CACAGATTGA 4681 ACGCTATTAC AAAAGCTGCG TTGAATTTCC GGATATGAAC AGCAGCCTGG AAGTTAAACG 4741 TAGTGAACTG GCACGTATGA TCAAAAACAT CAGCTTCGAC GATTTCAAGA ACGTTAAACA 4801 GCAGGCAAAA GGTCGTGAAA ATGTTGCAAA AGAACGTGCC AAAGCAGTGA TTGGTCTGTA 4861 TCTGACCGTT ATGTATCTGC TGGTTAAAAA TCTGGTTAAC GTGAATGCCC GTTATGTGAT 4921 TGCAATTCAT TGTCTGGAAC GTGATTTCGG CCTGTATAAA GAAATCATTC CGGAACTGGC 4981 AAGCAAGAAC CTGAAAAATG ATTATCGTAT TCTGAGTCAG ACCCTGTGTG AACTGTGTGA 5041 TAAAAGCCCG AACCTGTTTC TGAAGAAAAA CGAACGTCTG CGCAAATGCG TTGAGGTGGA 5101 TATTAACAAT GCAGATAGCA GCATGACCCG CAAATATCGT AATTGTATTG CACATCTGAC 5161 AGTTGTGCGC GAACTGAAAG AATATATTGG TGATATTCGT ACCGTGGACA GCTACTTTAG 5221 CATCTATCAT TATGTTATGC AGCGGTGTAT TACCAAACGC GAAAATGATA CCAAACAAGA 5281 AGAGAAAATC AAATACGAGG ACGATCTGCT TAAAAACCAC GGCTATACCA AAGATTTTGT 5341 GAAAGCCCTG AATAGCCCGT TCGGTTATAA CATTCCGCGT TTCAAAAATC TGAGCATCGA 5401 GCAGCTGTTT GATCGTAATG AATATCTGAC GGAAAAATAA TAAgaattcg gatccctcga 5461 gTTGACAGCT AGCTCAGTCC TAGGTATAAT GCTAGCAGAG ACCATGGCCA CCGGTCTCTG 5521 CTAAAGATAT GAGGAGCTCA AActcgagct gctaacaaag cccgaaagga agctgagttg 5581 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg 5641 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta 5701 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 5761 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 5821 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 5881 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 5941 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 6001 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 6061 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 6121 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 6181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag 6241 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca 6301 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg 6361 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt 6421 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa 6481 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca 6541 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc 6601 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc 6661 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct 6721 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca 6781 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt 6841 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac 6901 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta 6961 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta 7021 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa 7081 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 7141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 7201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 7261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 7321 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 7381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 7441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 7501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 7561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 7621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 7681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 7741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 7801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 7861 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 7921 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg 7981 cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag 8041 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc 8101 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gct //