LOCUS       pCI-globin_del5UTR_WT-xrRNA-4H      7614 bp    DNA     circular SYN 23-JUN-2000
DEFINITION  Cloning vector pCI-neo, mammalian expression vector, complete
            sequence.
ACCESSION   U47120
VERSION     U47120.2  GI:8677398
KEYWORDS    .
SOURCE      Cloning vector pCI-neo (unknown)
  ORGANISM  Cloning vector pCI-neo
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Brondyk,W.H.
  TITLE     New Mammalian Expression Vector with a selectable marker: pCI-neo
  JOURNAL   Promega Notes 51, 10-14 (1995)
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Brondyk,W.H. and Groskreutz,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5472)
  AUTHORS   Kamal,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2000) Technical Writing, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
   REMARK   Sequence updated by submitter
COMMENT     On Jun 23, 2000 this sequence version replaced gi:1197674.
FEATURES             Location/Qualifiers
     regulatory      1..659
                     /regulatory_class="enhancer"
                     /note="Cytomegalovirus immediate-early enhancer"
     Vector          join(3274..7614,1..1085)
                     /origin="CVU47120 (5.472bp circular)Target vector: from
                     NotI cut at 1131^1132 to NheI cut at 1085^1086,
                     5.426bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     regulatory      669..750
                     /regulatory_class="promoter"
                     /note="Cytomegalovirus immediate-early promoter"
     intron          890..1022
                     /note="chimeric intron"
     regulatory      1067..1085
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     exon            1094..1185
                     /label="exon1"
     CDS             join(1094..1185,1316..1538,2389..2517)
                     /label="globin"
     intron          1186..1315
                     /label="intron1"
     exon            1316..1538
                     /label="exon2"
     intron          1539..2388
                     /label="intron2"
     exon            2389..2517
                     /label="exon3"
     misc_feature    2605..2682
                     /label="MVE xrRNA1"
     misc_feature    2767..2832
                     /label="MVE xrRNA2"
     Site            2844..2943
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            2950..3049
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            3056..3155
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            3162..3261
                     /site_type="binding site"
                     /label="HBB binding site"
     regulatory      3282..3300
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     regulatory      3309..3530
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     Region          3428..3433
                     /label="polyA signal"
     Region          3445
                     /label="polyA cleavage"
     rep_origin      3625..4080
                     /note="phage f1 region"
     regulatory      4144..4560
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter - neomycin
                     selectable marker"
     rep_origin      4458..4523
                     /note="SV40 minimum origin of replication - neomycin
                     selectable marker"
     CDS             4605..5399
                     /gene="NeoR"
                     /function="neomycin resistance"
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /protein_id="AAA88781.1"
                     /db_xref="GI:1197675"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            4605..5399
                     /gene="NeoR"
     regulatory      5463..5511
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic polyadenylation signal - neomycin
                     selectable marker"
     CDS             5922..6782
                     /gene="ampR"
                     /function="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="AAA88782.1"
                     /db_xref="GI:1197676"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            5922..6782
                     /gene="ampR"
ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG
      661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
      721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC
      781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT
      841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA
      901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT
      961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC
     1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT
     1081 ATAGGCTAGC ACCATGGTGC ACCTGACTCC TGAGGAGAAG TCTGCCGTTA CTGCCCTGTG
     1141 GGGCAAGGTG AACGTGGATG AAGTTGGTGG TGAGGCCCTG GGCAGGTTGG TATCAAGGTT
     1201 ACAAGACAGG TTTAAGGAGA CCAATAGAAA CTGGGCATGT GGAGACAGAG AAGACTCTTG
     1261 GGTTTCTGAT AGGCACTGAC TCTCTCTGCC TATTGGTCTA TTTTCCCACC CTTAGGCTGC
     1321 TGGTGGTCTA CCCTTGGACC CAGAGGTTCT TTGAGTCCTT TGGGGATCTG TCCACTCCTG
     1381 ATGCTGTTAT GGGCAACCCT AAGGTGAAGG CTCATGGCAA GAAAGTGCTC GGTGCCTTTA
     1441 GTGATGGCCT GGCTCACCTG GACAACCTCA AGGGCACCTT TGCCACACTG AGTGAGCTGC
     1501 ACTGTGACAA GCTGCACGTG GATCCTGAGA ACTTCAGGGT GAGTCTATGG GACCCTTGAT
     1561 GTTTTCTTTC CCCTTCTTTT CTATGGTTAA GTTCATGTCA TAGGAAGGGG AGAAGTAACA
     1621 GGGTACAGTT TAGAATGGGA AACAGACGAA TGATTGCATC AGTGTGGAAG TCTCAGGATC
     1681 GTTTTAGTTT CTTTTATTTG CTGTTCATAA CAATTGTTTT CTTTTGTTTA ATTCTTGCTT
     1741 TCTTTTTTTT TCTTCTCCGC AATTTTTACT ATTATACTTA ATGCCTTAAC ATTGTGTATA
     1801 ACAAAAGGAA ATATCTCTGA GATACATTAA GTAACTTAAA AAAAAACTTT ACACAGTCTG
     1861 CCTAGTACAT TACTATTTGG AATATATGTG TGCTTATTTG CATATTCATA ATCTCCCTAC
     1921 TTTATTTTCT TTTATTTTTA ATTGATACAT AATCATTATA CATATTTATG GGTTAAAGTG
     1981 TAATGTTTTA ATATGTGTAC ACATATTGAC CAAATCAGGG TAATTTTGCA TTTGTAATTT
     2041 TAAAAAATGC TTTCTTCTTT TAATATACTT TTTTGTTTAT CTTATTTCTA ATACTTTCCC
     2101 TAATCTCTTT CTTTCAGGGC AATAATGATA CAATGTATCA TGCCTCTTTG CACCATTCTA
     2161 AAGAATAACA GTGATAATTT CTGGGTTAAG GCAATAGCAA TATTTCTGCA TATAAATATT
     2221 TCTGCATATA AATTGTAACT GATGTAAGAG GTTTCATATT GCTAATAGCA GCTACAATCC
     2281 AGCTACCATT CTGCTTTTAT TTTATGGTTG GGATAAGGCT GGATTATTCT GAGTCCAAGC
     2341 TAGGCCCTTT TGCTAATCAT GTTCATACCT CTTATCTTCC TCCCACAGCT CCTGGGCAAC
     2401 GTGCTGGTCT GTGTGCTGGC CCATCACTTT GGCAAAGAAT TCACCCCACC AGTGCAGGCT
     2461 GCCTATCAGA AAGTGGTGGC TGGTGTGGCT AATGCCCTGG CCCACAAGTA TCACTAAGCT
     2521 CGCTTTCTTG CTGTCCAATT TCTCTCGAGg tgagcaaggg cgaggagctg ttcaccgggg
     2581 tggtgcccat cctggtGgaC TTGATAGTCA GGCCAGCCGG TTAGGCTGCC ACCGAAGGTT
     2641 GGTAGACGGT GCTGCCTGCG ACCAACCCCA GGAGGACTGG GTTACCAAAG CTGATTCTCC
     2701 ACGGTTGGAA AGCCTCCCAG AACCGTCTCG GAAGAGGAGT CCCTGCCAAC AATGGAGATG
     2761 AAGCCCGTGT CAGATCGCGA AAGCGCCACT TCGCCGAGGA GTGCAATCTG TGAGGCCCCA
     2821 GGAGGACTGG GTAAACAGTC GAGCACCCCA CCAGTGCAGG CTGCCTATCA GAAAGTGGTG
     2881 GCTGGTGTGG CTAATGCCCT GGCCCACAAG TATCACTAAG CTCGCTTTCT TGCTGTCCAA
     2941 TTTGTCGAGC ACCCCACCAG TGCAGGCTGC CTATCAGAAA GTGGTGGCTG GTGTGGCTAA
     3001 TGCCCTGGCC CACAAGTATC ACTAAGCTCG CTTTCTTGCT GTCCAATTTG TCGAGCACCC
     3061 CACCAGTGCA GGCTGCCTAT CAGAAAGTGG TGGCTGGTGT GGCTAATGCC CTGGCCCACA
     3121 AGTATCACTA AGCTCGCTTT CTTGCTGTCC AATTTGTCGA GCACCCCACC AGTGCAGGCT
     3181 GCCTATCAGA AAGTGGTGGC TGGTGTGGCT AATGCCCTGG CCCACAAGTA TCACTAAGCT
     3241 CGCTTTCTTG CTGTCCAATT TGTCGACCCG GGCGGCCGCT TCCCTTTAGT GAGGGTTAAT
     3301 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA
     3361 GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT
     3421 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
     3481 GGAGATGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCCGATA
     3541 AGGATCGATC CGGGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG
     3601 TTGCGCAGCC TGAATGGCGA ATGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG
     3661 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG
     3721 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG
     3781 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT
     3841 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT
     3901 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA
     3961 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA
     4021 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT
     4081 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TACGCGGATC
     4141 TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGG TACCTTCTGA
     4201 GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC
     4261 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG
     4321 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
     4381 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
     4441 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
     4501 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT
     4561 GATTCTTCTG ACACAACAGT CTCGAACTTA AGGCTAGAGC CACCATGATT GAACAAGATG
     4621 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC
     4681 AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG
     4741 TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC
     4801 GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG
     4861 AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC
     4921 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC
     4981 TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA
     5041 CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG
     5101 CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG
     5161 TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT
     5221 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC
     5281 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA
     5341 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG
     5401 CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGATGGCC
     5461 GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG
     5521 CGATAAGGAT CCGCGTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA
     5581 GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
     5641 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
     5701 CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA
     5761 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     5821 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     5881 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
     5941 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
     6001 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
     6061 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
     6121 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG
     6181 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
     6241 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
     6301 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
     6361 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
     6421 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA
     6481 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
     6541 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
     6601 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
     6661 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
     6721 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
     6781 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
     6841 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
     6901 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     6961 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     7021 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     7081 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     7141 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     7201 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     7261 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     7321 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     7381 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     7441 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     7501 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     7561 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GGCTCGACAG ATCT
//
