LOCUS       pCI-TPI_WT-xrRNA-4H      8785 bp    DNA     circular SYN 23-JUN-2000
DEFINITION  Cloning vector pCI-neo, mammalian expression vector, complete
            sequence.
ACCESSION   U47120
VERSION     U47120.2  GI:8677398
KEYWORDS    .
SOURCE      Cloning vector pCI-neo (unknown)
  ORGANISM  Cloning vector pCI-neo
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Brondyk,W.H.
  TITLE     New Mammalian Expression Vector with a selectable marker: pCI-neo
  JOURNAL   Promega Notes 51, 10-14 (1995)
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Brondyk,W.H. and Groskreutz,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5472)
  AUTHORS   Kamal,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2000) Technical Writing, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
   REMARK   Sequence updated by submitter
COMMENT     On Jun 23, 2000 this sequence version replaced gi:1197674.
FEATURES             Location/Qualifiers
     regulatory      1..659
                     /regulatory_class="enhancer"
                     /note="Cytomegalovirus immediate-early enhancer"
     Vector          join(4445..8785,1..1085)
                     /origin="CVU47120 (5.472bp circular)Target vector: from
                     NotI cut at 1131^1132 to NheI cut at 1085^1086,
                     5.426bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
     regulatory      669..750
                     /regulatory_class="promoter"
                     /note="Cytomegalovirus immediate-early promoter"
     intron          890..1022
                     /note="chimeric intron"
     regulatory      1067..1085
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     exon            <1086..1222
                     /label="exon 1"
     CDS             join(1108..1222,2155..2278,2390..2474,2549..2681,2979..3064,3340..3427,3556..3674)
                     /label="TPI"
     intron          1223..2154
                     /label="intron 1"
     exon            2155..2278
                     /label="exon 2"
     intron          2279..2389
                     /label="intron 2"
     exon            2390..2474
                     /label="exon 3"
     intron          2475..2548
                     /label="intron 3"
     exon            2549..2681
                     /label="exon 4"
     intron          2682..2978
                     /label="intron 4"
     exon            2979..3064
                     /label="exon 5"
     intron          3065..3339
                     /label="intron 5"
     exon            3340..3427
                     /label="exon 6"
     intron          3428..3555
                     /label="intron 6"
     exon            3556..3674
                     /label="exon 7"
     misc_feature    3776..3853
                     /label="MVE xrRNA1"
     misc_feature    3938..4003
                     /label="MVE xrRNA2"
     Site            4015..4114
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            4121..4220
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            4227..4326
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            4333..4432
                     /site_type="binding site"
                     /label="HBB binding site"
     regulatory      4453..4471
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     regulatory      4480..4701
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     Region          4599..4604
                     /label="polyA signal"
     Region          4616
                     /label="polyA cleavage"
     rep_origin      4796..5251
                     /note="phage f1 region"
     regulatory      5315..5731
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter - neomycin
                     selectable marker"
     rep_origin      5629..5694
                     /note="SV40 minimum origin of replication - neomycin
                     selectable marker"
     CDS             5776..6570
                     /gene="NeoR"
                     /function="neomycin resistance"
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /protein_id="AAA88781.1"
                     /db_xref="GI:1197675"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            5776..6570
                     /gene="NeoR"
     regulatory      6634..6682
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic polyadenylation signal - neomycin
                     selectable marker"
     CDS             7093..7953
                     /gene="ampR"
                     /function="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="AAA88782.1"
                     /db_xref="GI:1197676"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            7093..7953
                     /gene="ampR"
ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG
      661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
      721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC
      781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT
      841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA
      901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT
      961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC
     1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT
     1081 ATAGGCTAGC GCCTCGGCTC CAGCACCATG Gcgccctcca ggaagttctt cgttggggga
     1141 aactggaaga tgaacgggcg gaagcagagt ctgggggagc tcatcggcac tctgaacgcg
     1201 gccaaggtgc cggccgacac cggtaagccc tcgccgagga ggggtctggc cgggccgggg
     1261 ccggggccgg ggcaggagtg gcagcgccct ctcccgaggc cccgaggccc cgaggccggt
     1321 atccgcgcgg acctgatgca gggctgtggg acgagggccg ctggggtccg ggcaggggcc
     1381 tcgcagccgc agccccgtcg gtgcgtcgag ggggcagggc ggagcacatg atgccccttg
     1441 gactatgggg caggtaagga cgttttcttc ccttcggcaa cctgaacgac tcccgccttc
     1501 cacggaaggg accgagcccg tgccaaacag gctgagcgat ttgggagtga ggagccatcc
     1561 taccgctttc cccaacctgg aaacagcaaa gcgcaaggcc tctgagtcag ttaggtctct
     1621 gccacccacg ggcaaaggat gctctcctcc atcctccttc ctccctccac cgaaatcgga
     1681 gagccgcggg cctgatccaa agaggcatcc ccttctcgtt cattccccag aggcctcaat
     1741 acaaacccca ggagttggcc cctctccttt tgctacaaat ccttgccttg caaaggggag
     1801 gtgaggatgg gctattttag aagggaagca gggttgctcc ctggagaatg ctgagtctgt
     1861 gaggtgccta tgccgagaat aggtcgagga aattggagcc ccagctgtta aaagagcaga
     1921 gggcagggtg agggccgtgg cctctcaggg gtatctggaa ggctcttcga gttgagtgca
     1981 gacccagcct gggctggaaa atggacaaag gtcatcttgc tggggtgaaa agggggagag
     2041 cagaaccaag aagaagaggg tgagggctgg ggggctccag ggcactggtt aggaattgtg
     2101 gggaatgaag gctttcttta gtctcatccc cctgtggtac catcttgtcc tcagaggtgg
     2161 tttgtgctcc ccctactgcc tatatcgact tcgcccggca gaagctagat cccaagattg
     2221 ctgtggctgc gcagaactgc tacaaagtga ctaatggggc ttttactggg gagatcaggt
     2281 gagatcgagg tggagagggg tgtgtgggac ccttccctca ctttcctcgt tgaggggaaa
     2341 gccacagggt gggctccctg ctgaaccttg gcttcatctc ttcctttagc cctggcatga
     2401 tcaaagactg cggagccacg tgggtggtcc tggggcactc agagagaagg catgtctttg
     2461 gggagtcaga tgaggttagt agccaagaga gaagataagg gatgtctttt tccaagaagg
     2521 atgtctcacc aagtctgttt ctcaacagct gattgggcag aaagtggccc atgctctggc
     2581 agagggactc ggagtaatcg cctgcattgg ggagaagcta gatgaaaggg aagctggcat
     2641 cactgagaag gttgttttcg agcagacaaa ggtcatcgca ggtatctctg gagaaaggga
     2701 cctttgagcc tatccagggc cacagagact cagagggtag ggtcaggccc tggagcctgt
     2761 cttggtcccc atgctgatcc agaaaaggaa aaaggggagg gggagtgaca atctttgctt
     2821 ggggcctatg acttctccag ccccaaggta gatgccacct ggaaatcccc caatgtccac
     2881 tagggggcag taggccaccg ttcttcgtac tccggagaac ctggctggag agctctttct
     2941 tgttcaccct tccctccatc tgtatctctg ccctgcagat aacgtgaagg actggagcaa
     3001 ggtcgtcctg gcctatgagc ctgtgtgggc cattggtact ggcaagactg caacacccca
     3061 acaggtaacc gggcccagga gccctgccct catcccagcc tgcctcaata ggtttggaca
     3121 gacacagccc acatggggca accccttatt tcaaagacac agagaccttg aacccagaga
     3181 cagtgacttg tccaagggca tccagtccag ggcctggctt ggatcagagc cctggtactc
     3241 tgactcagtc agaaaccaca ctaagtgtcc actggtgcca gtgatttttc ctcttagaga
     3301 ggcagaaaag gtcttactta ggccagcttc ttgttctagg cccaggaagt acacgagaag
     3361 ctccgaggat ggctgaagtc caacgtctct gatgcggtgg ctcagagcac ccgtatcatt
     3421 tatggaggtg agtggctttg gttcccggct gaggtggagt gggctgagga ctagactgag
     3481 ccctcggaca tggaggtggg gatggggcag actcatccca ttcttgacca agcccttgtt
     3541 ctgctccctt cccagGCTCT GTGACTGGGG CAACCTGCAA GGAGCTGGCC AGCCAGCCTG
     3601 ATGTGGATGG CTTCCTTGTG GGTGGTGCTT CCCTCAAGCC CGAATTCGTG GACATCATCA
     3661 ATGCCAAACA ATGAGCCCCA TCCATCTTCC CTACCCTTCC TGCCAAGCCA GGGACTCGAG
     3721 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtGga CTTGATAGTC
     3781 AGGCCAGCCG GTTAGGCTGC CACCGAAGGT TGGTAGACGG TGCTGCCTGC GACCAACCCC
     3841 AGGAGGACTG GGTTACCAAA GCTGATTCTC CACGGTTGGA AAGCCTCCCA GAACCGTCTC
     3901 GGAAGAGGAG TCCCTGCCAA CAATGGAGAT GAAGCCCGTG TCAGATCGCG AAAGCGCCAC
     3961 TTCGCCGAGG AGTGCAATCT GTGAGGCCCC AGGAGGACTG GGTAAACAGT CGAGCACCCC
     4021 ACCAGTGCAG GCTGCCTATC AGAAAGTGGT GGCTGGTGTG GCTAATGCCC TGGCCCACAA
     4081 GTATCACTAA GCTCGCTTTC TTGCTGTCCA ATTTGTCGAG CACCCCACCA GTGCAGGCTG
     4141 CCTATCAGAA AGTGGTGGCT GGTGTGGCTA ATGCCCTGGC CCACAAGTAT CACTAAGCTC
     4201 GCTTTCTTGC TGTCCAATTT GTCGAGCACC CCACCAGTGC AGGCTGCCTA TCAGAAAGTG
     4261 GTGGCTGGTG TGGCTAATGC CCTGGCCCAC AAGTATCACT AAGCTCGCTT TCTTGCTGTC
     4321 CAATTTGTCG AGCACCCCAC CAGTGCAGGC TGCCTATCAG AAAGTGGTGG CTGGTGTGGC
     4381 TAATGCCCTG GCCCACAAGT ATCACTAAGC TCGCTTTCTT GCTGTCCAAT TTGTCGACCC
     4441 GGGCGGCCGC TTCCCTTTAG TGAGGGTTAA TGCTTCGAGC AGACATGATA AGATACATTG
     4501 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT
     4561 GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA
     4621 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TAAAGCAAGT
     4681 AAAACCTCTA CAAATGTGGT AAAATCCGAT AAGGATCGAT CCGGGCTGGC GTAATAGCGA
     4741 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGACGCG
     4801 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
     4861 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
     4921 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
     4981 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
     5041 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
     5101 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG
     5161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG
     5221 AATTTTAACA AAATATTAAC GCTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC
     5281 TGTGCGGTAT TTCACACCGC ATACGCGGAT CTGCGCAGCA CCATGGCCTG AAATAACCTC
     5341 TGAAAGAGGA ACTTGGTTAG GTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG
     5401 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
     5461 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT
     5521 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC
     5581 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT
     5641 TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT
     5701 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TGATTCTTCT GACACAACAG TCTCGAACTT
     5761 AAGGCTAGAG CCACCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG
     5821 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC
     5881 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT
     5941 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT
     6001 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
     6061 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC
     6121 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC
     6181 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG
     6241 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG
     6301 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
     6361 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
     6421 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
     6481 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC
     6541 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC
     6601 AAGCGACGCC CAACCTGCCA TCACGATGGC CGCAATAAAA TATCTTTATT TTCATTACAT
     6661 CTGTGTGTTG GTTTTTTGTG TGAATCGATA GCGATAAGGA TCCGCGTATG GTGCACTCTC
     6721 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT
     6781 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC
     6841 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG
     6901 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG
     6961 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA
     7021 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
     7081 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA
     7141 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT
     7201 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG
     7261 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC
     7321 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT
     7381 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA
     7441 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT
     7501 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT
     7561 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT
     7621 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA
     7681 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA
     7741 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT
     7801 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC
     7861 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT
     7921 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA
     7981 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT
     8041 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC
     8101 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG
     8161 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
     8221 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG
     8281 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG
     8341 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC
     8401 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA
     8461 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA
     8521 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC
     8581 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT
     8641 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG
     8701 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT
     8761 TTTGCTCACA TGGCTCGACA GATCT
//
