LOCUS       pcDNA5_FRT_TO-TPI_PTC160-xrRNA-4H      8426 bp    DNA     circular SYN 01-NOV-2010
DEFINITION  
ACCESSION   pcDNA5/FRT/TO-TOPO
DBSOURCE    Private
SOURCE      Homo sapiens (unknown)
  ORGANISM  Homo sapiens
            Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo
COMMENT     This file is created by VectorDesigner
FEATURES             Location/Qualifiers
     primer          complement(35..52)
                     /invitrogen="1910000"
                     /note="Oriinal location: complement(118..135)"
                     /label="BGH reverse primer"
     polyA_site      41..265
                     /invitrogen="1870000"
                     /note="Oriinal location: 124..348"
                     /label="BGH pA"
     misc_recomb     549..596
                     /invitrogen="2710000"
                     /note="Oriinal location: 632..679"
                     /label="FRT"
     CDS             604..1626
                     /invitrogen="1190000"
                     /note="Oriinal location: 687..1709"
                     /label="Hygro(R) (no ATG)"
     polyA_signal    1756..1886
                     /invitrogen="1840000"
                     /note="Oriinal location: 1839..1969"
                     /label="SV40 pA"
     rep_origin      complement(2269..2942)
                     /invitrogen="2540000"
                     /note="Oriinal location: complement(2352..3025)"
                     /label="pUC origin"
     CDS             complement(3087..3947)
                     /invitrogen="1020000"
                     /note="Oriinal location: complement(3170..4030)"
                     /label="Amp(R)"
     promoter        complement(3948..4046)
                     /invitrogen="2330000"
                     /note="Oriinal location: complement(4031..4129)"
                     /label="bla promoter"
     promoter        4315..5041
                     /invitrogen="2200000"
                     /note="Oriinal location: 4398..5124"
                     /label="CMV promoter (with TetOs)"
     primer          4852..4872
                     /invitrogen="1930000"
                     /note="Oriinal location: 4935..4955"
                     /label="CMV forward primer"
     TATA_signal     4887..4893
                     /invitrogen="123000002"
                     /note="Oriinal location: 4970..4976"
                     /label="TATA box"
     protein_bind    4903..4942
                     /invitrogen="2450000"
                     /note="Oriinal location: 4986..5025"
                     /label="TetR binding sites"
     exon            <5068..5204
                     /label="exon 1"
     CDS             join(5090..5204,6137..6260,6372..6456,6531..6663,6961..7046,7322..7409,7538..7656)
                     /label="TPI"
     intron          5205..6136
                     /label="intron 1"
     exon            6137..6260
                     /label="exon 2"
     intron          6261..6371
                     /label="intron 2"
     exon            6372..6456
                     /label="exon 3"
     intron          6457..6530
                     /label="intron 3"
     exon            6531..6663
                     /label="exon 4"
     intron          6664..6960
                     /label="intron 4"
     exon            6961..7046
                     /label="exon 5"
     mutation        6981..6983
                     /label="PTC160"
     intron          7047..7321
                     /label="intron 5"
     exon            7322..7409
                     /label="exon 6"
     intron          7410..7537
                     /label="intron 6"
     exon            7538..7656
                     /label="exon 7"
     misc_feature    7758..7835
                     /label="MVE xrRNA1"
     misc_feature    7920..7985
                     /label="MVE xrRNA2"
     Site            7997..8096
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            8103..8202
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            8209..8308
                     /site_type="binding site"
                     /label="HBB binding site"
     Site            8315..8414
                     /site_type="binding site"
                     /label="HBB binding site"
ORIGIN
        1 GGCCGCAGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG
       61 CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT
      121 GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT
      181 CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT
      241 GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG
      301 GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
      361 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
      421 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
      481 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
      541 CGTACCTAGA AGTTCCTATT CCGAAGTTCC TATTCTCTAG AAAGTATAGG AACTTCCTTG
      601 GCCAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC
      661 AGCGTCTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT
      721 GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT
      781 CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT
      841 GGGGAATTCA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG
      901 CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT
      961 GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA
     1021 ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT
     1081 CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG
     1141 CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC
     1201 TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG
     1261 ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT
     1321 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
     1381 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
     1441 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
     1501 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
     1561 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
     1621 GAATAGCACG TACTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
     1681 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
     1741 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
     1801 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
     1861 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
     1921 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
     1981 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
     2041 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
     2101 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
     2161 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
     2221 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
     2281 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
     2341 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
     2401 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
     2461 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
     2521 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
     2581 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
     2641 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
     2701 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
     2761 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
     2821 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
     2881 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
     2941 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     3001 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     3061 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     3121 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     3181 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
     3241 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     3301 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     3361 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     3421 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     3481 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     3541 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     3601 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     3661 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     3721 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     3781 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     3841 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3901 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3961 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     4021 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
     4081 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
     4141 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
     4201 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
     4261 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
     4321 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
     4381 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
     4441 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
     4501 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
     4561 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
     4621 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
     4681 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
     4741 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
     4801 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
     4861 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCTCCCTATC AGTGATAGAG
     4921 ATCTCCCTAT CAGTGATAGA GATCGTCGAC GAGCTCGTTT AGTGAACCGT CAGATCGCCT
     4981 GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC
     5041 GGACTCTAGC GTTTAAACTT AAGCTTGCTA GCGCCTCGGC TCCAGCACCA TGGcgccctc
     5101 caggaagttc ttcgttgggg gaaactggaa gatgaacggg cggaagcaga gtctggggga
     5161 gctcatcggc actctgaacg cggccaaggt gccggccgac accggtaagc cctcgccgag
     5221 gaggggtctg gccgggccgg ggccggggcc ggggcaggag tggcagcgcc ctctcccgag
     5281 gccccgaggc cccgaggccg gtatccgcgc ggacctgatg cagggctgtg ggacgagggc
     5341 cgctggggtc cgggcagggg cctcgcagcc gcagccccgt cggtgcgtcg agggggcagg
     5401 gcggagcaca tgatgcccct tggactatgg ggcaggtaag gacgttttct tcccttcggc
     5461 aacctgaacg actcccgcct tccacggaag ggaccgagcc cgtgccaaac aggctgagcg
     5521 atttgggagt gaggagccat cctaccgctt tccccaacct ggaaacagca aagcgcaagg
     5581 cctctgagtc agttaggtct ctgccaccca cgggcaaagg atgctctcct ccatcctcct
     5641 tcctccctcc accgaaatcg gagagccgcg ggcctgatcc aaagaggcat ccccttctcg
     5701 ttcattcccc agaggcctca atacaaaccc caggagttgg cccctctcct tttgctacaa
     5761 atccttgcct tgcaaagggg aggtgaggat gggctatttt agaagggaag cagggttgct
     5821 ccctggagaa tgctgagtct gtgaggtgcc tatgccgaga ataggtcgag gaaattggag
     5881 ccccagctgt taaaagagca gagggcaggg tgagggccgt ggcctctcag gggtatctgg
     5941 aaggctcttc gagttgagtg cagacccagc ctgggctgga aaatggacaa aggtcatctt
     6001 gctggggtga aaagggggag agcagaacca agaagaagag ggtgagggct ggggggctcc
     6061 agggcactgg ttaggaattg tggggaatga aggctttctt tagtctcatc cccctgtggt
     6121 accatcttgt cctcagaggt ggtttgtgct ccccctactg cctatatcga cttcgcccgg
     6181 cagaagctag atcccaagat tgctgtggct gcgcagaact gctacaaagt gactaatggg
     6241 gcttttactg gggagatcag gtgagatcga ggtggagagg ggtgtgtggg acccttccct
     6301 cactttcctc gttgagggga aagccacagg gtgggctccc tgctgaacct tggcttcatc
     6361 tcttccttta gccctggcat gatcaaagac tgcggagcca cgtgggtggt cctggggcac
     6421 tcagagagaa ggcatgtctt tggggagtca gatgaggtta gtagccaaga gagaagataa
     6481 gggatgtctt tttccaagaa ggatgtctca ccaagtctgt ttctcaacag ctgattgggc
     6541 agaaagtggc ccatgctctg gcagagggac tcggagtaat cgcctgcatt ggggagaagc
     6601 tagatgaaag ggaagctggc atcactgaga aggttgtttt cgagcagaca aaggtcatcg
     6661 caggtatctc tggagaaagg gacctttgag cctatccagg gccacagaga ctcagagggt
     6721 agggtcaggc cctggagcct gtcttggtcc ccatgctgat ccagaaaagg aaaaagggga
     6781 gggggagtga caatctttgc ttggggccta tgacttctcc agccccaagg tagatgccac
     6841 ctggaaatcc cccaatgtcc actagggggc agtaggccac cgttcttcgt actccggaga
     6901 acctggctgg agagctcttt cttgttcacc cttccctcca tctgtatctc tgccctgcag
     6961 ataacgtgaa ggactggagc taagtcgtcc tggcctatga gcctgtgtgg gccattggta
     7021 ctggcaagac tgcaacaccc caacaggtaa ccgggcccag gagccctgcc ctcatcccag
     7081 cctgcctcaa taggtttgga cagacacagc ccacatgggg caacccctta tttcaaagac
     7141 acagagacct tgaacccaga gacagtgact tgtccaaggg catccagtcc agggcctggc
     7201 ttggatcaga gccctggtac tctgactcag tcagaaacca cactaagtgt ccactggtgc
     7261 cagtgatttt tcctcttaga gaggcagaaa aggtcttact taggccagct tcttgttcta
     7321 ggcccaggaa gtacacgaga agctccgagg atggctgaag tccaacgtct ctgatgcggt
     7381 ggctcagagc acccgtatca tttatggagg tgagtggctt tggttcccgg ctgaggtgga
     7441 gtgggctgag gactagactg agccctcgga catggaggtg gggatggggc agactcatcc
     7501 cattcttgac caagcccttg ttctgctccc ttcccagGCT CTGTGACTGG GGCAACCTGC
     7561 AAGGAGCTGG CCAGCCAGCC TGATGTGGAT GGCTTCCTTG TGGGTGGTGC TTCCCTCAAG
     7621 CCCGAATTCG TGGACATCAT CAATGCCAAA CAATGAGCCC CATCCATCTT CCCTACCCTT
     7681 CCTGCCAAGC CAGGGACTCG AGgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7741 catcctggtG gaCTTGATAG TCAGGCCAGC CGGTTAGGCT GCCACCGAAG GTTGGTAGAC
     7801 GGTGCTGCCT GCGACCAACC CCAGGAGGAC TGGGTTACCA AAGCTGATTC TCCACGGTTG
     7861 GAAAGCCTCC CAGAACCGTC TCGGAAGAGG AGTCCCTGCC AACAATGGAG ATGAAGCCCG
     7921 TGTCAGATCG CGAAAGCGCC ACTTCGCCGA GGAGTGCAAT CTGTGAGGCC CCAGGAGGAC
     7981 TGGGTAAACA GTCGAGCACC CCACCAGTGC AGGCTGCCTA TCAGAAAGTG GTGGCTGGTG
     8041 TGGCTAATGC CCTGGCCCAC AAGTATCACT AAGCTCGCTT TCTTGCTGTC CAATTTGTCG
     8101 AGCACCCCAC CAGTGCAGGC TGCCTATCAG AAAGTGGTGG CTGGTGTGGC TAATGCCCTG
     8161 GCCCACAAGT ATCACTAAGC TCGCTTTCTT GCTGTCCAAT TTGTCGAGCA CCCCACCAGT
     8221 GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT GCCCTGGCCC ACAAGTATCA
     8281 CTAAGCTCGC TTTCTTGCTG TCCAATTTGT CGAGCACCCC ACCAGTGCAG GCTGCCTATC
     8341 AGAAAGTGGT GGCTGGTGTG GCTAATGCCC TGGCCCACAA GTATCACTAA GCTCGCTTTC
     8401 TTGCTGTCCA ATTTGTCGAC CCGGGC
//
