LOCUS xCas9(3.7)-BE4 8877 bp DNA circular UNA 26-FEB-2018 DEFINITION xBE4 3.6. ACCESSION urn.local...1in-8pm0hos VERSION urn.local...1in-8pm0hos KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers Promoter 275..356 /Imported_from="Pla sMapper" /Transferred_From="CMV prom" /Transferred_Similarity="97.56%" /label="CMV prom" Promoter 377..395 /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" CDS 409..1095 /created_by="User" /label="rAPOBEC1" CDS 1096..1119 /created_by="jhu" /label="2x SGGS linker" CDS 1120..1167 /created_by="User" /label="x ten " CDS 1168..1191 /created_by="jhu" /label="2x SGGS linker" CDS 1192..5292 /ApEinfo_fwdcolor="#916d92" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /modified_by="jhu" /label="xCas9 3.7 (D10A nickase)" misc_feature 1216..1218 /created_by="shannonmiller" /modified_by="jhu" /label="D10A" misc_feature 1972..1974 /created_by="jhu" /modified_by="jhu" /label="A262T" misc_feature 2158..2160 /created_by="jhu" /modified_by="jhu" /label="R324L" misc_feature 2413..2415 /created_by="jhu" /modified_by="jhu" /label="S409I" misc_feature 2626..2628 /created_by="jhu" /modified_by="jhu" /label="E480K" misc_feature 2815..2817 /created_by="jhu" /modified_by="jhu" /label="E543D" misc_feature 3268..3270 /created_by="jhu" /modified_by="jhu" /label="M694I" misc_feature 4843..4845 /created_by="jhu" /modified_by="jhu" /label="E1219V" CDS 5293..5322 /created_by="jhu" /label="2x SGGS linker" CDS 5323..5571 /created_by="User" /modified_by="jhu" /label="UGI" CDS 5572..5601 /created_by="jhu" /label="2x SGGS linker" CDS <5602..5850 /created_by="User" /label="UGI" CDS 5851..5862 /created_by="jhu" /label="SGGS linker" CDS 5863..5886 /created_by="User" /modified_by="jhu" /label="SV40 NLS" Terminator 5935..6162 /Imported_from="Pla sMapper" /Transferred_From="bGH_PA term" /Transferred_Similarity="100.00%" /label="bGH_PA term" misc_feature complement(6251..6278) /Imported_from="Pla sMapper" /Transferred_From="lacO reg" /Transferred_Similarity="89.29%" /label="lacO reg" /note="Geneious type: Regulatory Sequence" Promoter complement(6282..6311) /Imported_from="Pla sMapper" /Transferred_From="lac prom" /Transferred_Similarity="100.00%" /label="lac prom" misc_feature complement(6620..7239) /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" Marker complement(7394..8254) /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /label="amp marker" Promoter complement(8296..8324) /Imported_from="Pla sMapper" /Transferred_From="amp prom" /Transferred_Similarity="100.00%" /label="amp prom" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag 421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag 481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg 541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc 601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt 661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg 721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac 781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg 841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa 901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc 961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca 1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg 1081 gccaccgggt tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg 1141 acctcagagt ccgccacacc cgaaagttct ggtggttctt ctggtggttc tgacaagaag 1201 tactccattg ggctcgctat cggcacaaac agcgtcggct gggccgtcat tacggacgag 1261 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag 1321 aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc 1381 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag 1441 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc 1501 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac 1561 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac 1621 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt 1681 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc 1741 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc 1801 ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac 1861 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg 1921 tccctcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga taccaagctt 1981 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac 2041 cagtacgcag accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat 2101 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagctc 2161 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct 2221 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac 2281 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac 2341 ggcaccgagg agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact 2401 ttcgacaatg gaatcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg 2461 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc 2521 acatttcgga taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg 2581 atgactcgca aatcagaaga gaccatcact ccctggaact tcgagaaagt cgtggataag 2641 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac 2701 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc 2761 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagatcag 2821 aagaaagcta ttgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc 2881 aaagaagact atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag 2941 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag 3001 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg 3061 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac 3121 gacaaagtca tgaagcagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga 3181 aaactgatca atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag 3241 tccgatggat ttgccaaccg gaacttcatt cagttgatcc atgatgactc tctcaccttt 3301 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc 3361 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg 3421 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc 3481 cgagagaacc aaaccaccca gaagggacag aagaacagta gggaaaggat gaagaggatt 3541 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc 3601 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg 3661 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag 3721 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaacaga 3781 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg 3841 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa 3901 cgaggtggcc tgtctgagtt ggataaagcc ggtttcatca aaaggcagct tgttgagaca 3961 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat 4021 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca 4081 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg 4141 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt 4201 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag 4261 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat 4321 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa 4381 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg 4441 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc 4501 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa 4561 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg 4621 gttgtggcta aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg 4681 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg 4741 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt 4801 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgtgctgca gaaaggtaac 4861 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag 4921 ctcaaagggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac 4981 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac 5041 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag 5101 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc 5161 aagtacttcg acactaccat agacagaaag cggtacacct ctacaaagga ggtcctggac 5221 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag 5281 ctcggtggag actctggtgg ttctggagga tctggtggtt ctactaatct gtcagatatt 5341 attgaaaagg agaccggtaa gcaactggtt atccaggaat ccatcctcat gctcccagag 5401 gaggtggaag aagtcattgg gaacaagccg gaaagcgata tactcgtgca caccgcctac 5461 gacgagagca ccgacgagaa tgtcatgctt ctgactagcg acgcccctga atacaagcct 5521 tgggctctgg tcatacagga tagcaacggt gagaacaaga ttaagatgct ctctggtggt 5581 tctggaggat ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag 5641 caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg 5701 aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat 5761 gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat 5821 agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa 5881 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 5941 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 6001 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 6061 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 6121 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 6181 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6301 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8401 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 8461 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8521 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 8581 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 8641 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 8701 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 8761 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 8821 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc //