LOCUS xCas9(3.7)-BE4 8877 bp DNA circular UNA 26-FEB-2018
DEFINITION xBE4 3.6.
ACCESSION urn.local...1in-8pm0hos
VERSION urn.local...1in-8pm0hos
KEYWORDS .
SOURCE
ORGANISM
.
FEATURES Location/Qualifiers
Promoter 275..356
/Imported_from="Pla
sMapper"
/Transferred_From="CMV prom"
/Transferred_Similarity="97.56%"
/label="CMV prom"
Promoter 377..395
/Imported_from="Pla
sMapper"
/Transferred_From="T7 prom"
/Transferred_Similarity="100.00%"
/label="T7 prom"
CDS 409..1095
/created_by="User"
/label="rAPOBEC1"
CDS 1096..1119
/created_by="jhu"
/label="2x SGGS linker"
CDS 1120..1167
/created_by="User"
/label="x ten "
CDS 1168..1191
/created_by="jhu"
/label="2x SGGS linker"
CDS 1192..5292
/ApEinfo_fwdcolor="#916d92"
/ApEinfo_revcolor="green"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0"
/modified_by="jhu"
/label="xCas9 3.7 (D10A nickase)"
misc_feature 1216..1218
/created_by="shannonmiller"
/modified_by="jhu"
/label="D10A"
misc_feature 1972..1974
/created_by="jhu"
/modified_by="jhu"
/label="A262T"
misc_feature 2158..2160
/created_by="jhu"
/modified_by="jhu"
/label="R324L"
misc_feature 2413..2415
/created_by="jhu"
/modified_by="jhu"
/label="S409I"
misc_feature 2626..2628
/created_by="jhu"
/modified_by="jhu"
/label="E480K"
misc_feature 2815..2817
/created_by="jhu"
/modified_by="jhu"
/label="E543D"
misc_feature 3268..3270
/created_by="jhu"
/modified_by="jhu"
/label="M694I"
misc_feature 4843..4845
/created_by="jhu"
/modified_by="jhu"
/label="E1219V"
CDS 5293..5322
/created_by="jhu"
/label="2x SGGS linker"
CDS 5323..5571
/created_by="User"
/modified_by="jhu"
/label="UGI"
CDS 5572..5601
/created_by="jhu"
/label="2x SGGS linker"
CDS <5602..5850
/created_by="User"
/label="UGI"
CDS 5851..5862
/created_by="jhu"
/label="SGGS linker"
CDS 5863..5886
/created_by="User"
/modified_by="jhu"
/label="SV40 NLS"
Terminator 5935..6162
/Imported_from="Pla
sMapper"
/Transferred_From="bGH_PA term"
/Transferred_Similarity="100.00%"
/label="bGH_PA term"
misc_feature complement(6251..6278)
/Imported_from="Pla
sMapper"
/Transferred_From="lacO reg"
/Transferred_Similarity="89.29%"
/label="lacO reg"
/note="Geneious type: Regulatory Sequence"
Promoter complement(6282..6311)
/Imported_from="Pla
sMapper"
/Transferred_From="lac prom"
/Transferred_Similarity="100.00%"
/label="lac prom"
misc_feature complement(6620..7239)
/Imported_from="Pla
sMapper"
/Transferred_From="pBR322 origin"
/Transferred_Similarity="99.52%"
/label="pBR322 origin"
/note="Geneious type: Origin of Replication"
Marker complement(7394..8254)
/Imported_from="Pla
sMapper"
/Transferred_From="amp marker"
/Transferred_Similarity="99.88%"
/label="amp marker"
Promoter complement(8296..8324)
/Imported_from="Pla
sMapper"
/Transferred_From="amp prom"
/Transferred_Similarity="100.00%"
/label="amp prom"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag
421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
1081 gccaccgggt tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg
1141 acctcagagt ccgccacacc cgaaagttct ggtggttctt ctggtggttc tgacaagaag
1201 tactccattg ggctcgctat cggcacaaac agcgtcggct gggccgtcat tacggacgag
1261 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
1321 aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc
1381 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
1441 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
1501 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
1561 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac
1621 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt
1681 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
1741 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc
1801 ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac
1861 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
1921 tccctcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga taccaagctt
1981 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac
2041 cagtacgcag accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat
2101 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagctc
2161 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct
2221 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac
2281 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac
2341 ggcaccgagg agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact
2401 ttcgacaatg gaatcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg
2461 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc
2521 acatttcgga taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg
2581 atgactcgca aatcagaaga gaccatcact ccctggaact tcgagaaagt cgtggataag
2641 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac
2701 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc
2761 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagatcag
2821 aagaaagcta ttgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc
2881 aaagaagact atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag
2941 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag
3001 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg
3061 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac
3121 gacaaagtca tgaagcagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga
3181 aaactgatca atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag
3241 tccgatggat ttgccaaccg gaacttcatt cagttgatcc atgatgactc tctcaccttt
3301 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc
3361 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
3421 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
3481 cgagagaacc aaaccaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
3541 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
3601 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
3661 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag
3721 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaacaga
3781 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
3841 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
3901 cgaggtggcc tgtctgagtt ggataaagcc ggtttcatca aaaggcagct tgttgagaca
3961 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
4021 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca
4081 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
4141 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt
4201 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
4261 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
4321 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
4381 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg
4441 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc
4501 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
4561 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
4621 gttgtggcta aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
4681 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg
4741 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt
4801 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgtgctgca gaaaggtaac
4861 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
4921 ctcaaagggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
4981 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac
5041 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
5101 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
5161 aagtacttcg acactaccat agacagaaag cggtacacct ctacaaagga ggtcctggac
5221 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
5281 ctcggtggag actctggtgg ttctggagga tctggtggtt ctactaatct gtcagatatt
5341 attgaaaagg agaccggtaa gcaactggtt atccaggaat ccatcctcat gctcccagag
5401 gaggtggaag aagtcattgg gaacaagccg gaaagcgata tactcgtgca caccgcctac
5461 gacgagagca ccgacgagaa tgtcatgctt ctgactagcg acgcccctga atacaagcct
5521 tgggctctgg tcatacagga tagcaacggt gagaacaaga ttaagatgct ctctggtggt
5581 tctggaggat ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag
5641 caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg
5701 aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat
5761 gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat
5821 agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa
5881 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
5941 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
6001 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
6061 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
6121 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
6181 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
6301 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
8401 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
8461 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
8521 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
8581 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
8641 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
8701 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
8761 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
8821 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc
//