LOCUS       Exported                3004 bp ds-DNA     circular SYN 28-FEB-2018
DEFINITION  This plasmid is useful for in vitro transcription of genes using T7 
            promoter. Can be used for Xenopus oocyte assay. It is derived from 
            pT7TS. More sites for cloning are incorporated.
ACCESSION   .
VERSION     .
KEYWORDS    pT7TS-rich
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3004)
  TITLE     Belanger lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 28, 2018 from SnapGene 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(9..31)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(225..244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     344..366
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(404..422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        490..594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             595..1455
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(813..832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1626..2214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2115..2134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2368..2385
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2502..2523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2538..2568
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2576..2592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2581..2603
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2600..2616
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2600..2616
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2635..2654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2635..2653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2666..2708
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           2751..2876
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(2978..2996)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2979..2996)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3004..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3004..16)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
       61 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      121 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
      181 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
      241 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
      301 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
      361 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
      421 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2281 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2341 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2401 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2461 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2521 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2581 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctaatac
     2641 gactcactat agggagacaa gcttgcttgt tctttttgca gaagctcaga ataaacgctc
     2701 aactttggca gatctgtctt cactagctag tgaagacctg gttaccacta aaccagcctc
     2761 aagaacaccc gaatggagtc tctaagctac ataataccaa cttacacttt acaaaatgtt
     2821 gtcccccaaa atgtagccat tcgtatctgc tcctaataaa aagaaagttt cttcacattc
     2881 taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaccccc cccccccccc cctgcaggtc
     2941 gactctagag gatccccggg cgagctcgaa ttcgtattct atagtgtcac ctaaatccaa
     3001 ttca
//
