LOCUS Supplementary_Da 7593 bp ds-DNA circular 01-MAR-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Extended Data File 2 _gb SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7593) AUTHORS . TITLE Direct Submission JOURNAL Exported martes, 30 de ago de 2016 from SnapGene Viewer 3.0.3 http://www.snapgene.com COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 413..435 /note="SP6" /label=SP6 /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 439..1146 /note="mCherry" /label=mCherry /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1147..1212 /note="P2A" /label=P2A /ApEinfo_fwdcolor=#7eff74 /ApEinfo_revcolor=#7eff74 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1213..3198 /note="CreERT2" /label=CreERT2 /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#7eff74 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3226..3464 /note="SV40 poliA" /label=SV40 poliA /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3509..3543 /note="FRT" /label=FRT /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3798..3936 /note="SV40 poliA(1)" /label=SV40 poliA(1) /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3987..4779 /note="KanR" /label=KanR /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=#c0c0c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4860..4894 /note="FRT(1)" /label=FRT(1) /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctcggcgcg ccatttaggt 421 gacactatag aagagaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 481 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 541 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 601 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 661 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 721 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 781 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 841 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 901 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 961 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 1021 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 1081 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 1141 tacaagggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag 1201 aaccctggac ctgctagctc caacctgctg actgtgcacc aaaacctgcc tgccctccct 1261 gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag 1321 gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg 1381 tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc 1441 ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc 1501 aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg 1561 gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg 1621 gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc 1681 caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc 1741 gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc 1801 cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg 1861 ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac 1921 aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa 1981 ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc 2041 aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct 2101 gccagggaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg 2161 accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac tggggctatg 2221 gtgaggctgc ttgaggatgg ggacctcgag ccatctgctg gagacatgag agctgccaac 2281 ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg 2341 acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag 2401 tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca 2461 gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg 2521 accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt 2581 ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg 2641 gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct 2701 acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct 2761 attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa 2821 gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg 2881 gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc 2941 ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc 3001 aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat 3061 gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact 3121 gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt 3181 ttccctgcca cagcttgatg aagatctgag ctcgaattcg cccttaggcc tgcaggatca 3241 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 3301 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 3361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 3421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaactagg ggggatctgc 3481 tagcccggat ctagataact gatcagcttg aagttcctat actttctaga gaataggaac 3541 ttcggaatag gaacttcaag atccccctgg cgaaaggggg atgtgctgca aggcgattaa 3601 gttgggtaac gccagggttt tcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 3661 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 3721 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg 3781 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3841 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 3901 cagaagtatg caaagcatgc atctcaatta gtcagtaacc atagtccgca aagatcgatc 3961 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 4021 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 4081 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 4141 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 4201 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 4261 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 4321 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 4381 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 4441 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 4501 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 4561 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 4621 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 4681 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 4741 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatcgg caataaaaag 4801 acagaataaa acgcacgggt gttgggtcgt ttgttcggat ccgagcttca aaagcgctct 4861 gaagttccta tactttctag agaataggaa cttcggaata gtaacttctc catggtagcc 4921 tccaaaaaag cctcctcact acttctggac tagtgcggcc gccagagctg gccactgctt 4981 tgggagcctt cctgggcctc ctggcagtgg cagcaatggc aggccctaac tttccccaga 5041 tagacacccc caacatgcta cctgcccacc atcgccaaaa gagagactgg atttggaatc 5101 aaatgcacat cgatgaagag aaaaatgaat cgctgcccca ctatgtggga aaggtaagcc 5161 tcgcgctcca gggtgatggc cattatcaac actggaccat agtcatggcc atgtagccaa 5221 gatggtggtg aataagattg gttatcattg gtggtgcggg gggacggaga ggaggcaaag 5281 aacagggtgg taataatggt gaaagtgaca tgtgtgctga tggttgtgat aggaaagaga 5341 aattaattaa gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 5401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 5461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 5521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 5581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 5641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 5701 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 5761 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 5821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 5881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 5941 tcccttcggg aagcgtggcg ctttctcaaa gctcacgctg taggtatctc agttcggtgt 6001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6061 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 6121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 6181 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 6241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 6301 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 6361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 6421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 6481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 6541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 6601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 6661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 6721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 6781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 6841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 6901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 6961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 7381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 7441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 7501 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 7561 ataaaaatag gcgtatcacg aggccctttc gtc //