LOCUS       pAJM.712                2927 bp ds-DNA     circular     13-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513113063755.339
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     misc_feature    73..104
                     /note="Geneious type: operator"
                     /label="CymO"
     promoter        73..162
                     /label="pCymRC"
     promoter        96..101
                     /label="-35"
     promoter        119..124
                     /label="-10"
     misc_feature    131..162
                     /note="Geneious type: operator"
                     /label="CymO"
     promoter        131
                     /label="+1"
     misc_feature    163..237
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    246..257
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    264..983
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    984..1044
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     rep_origin      1104..1940
                     /label="more\stringent\p15A"
     rep_origin      1377
                     /label="origin"
     CDS             complement(2012..2827)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgaacaaaca gacaatctgg tctgtttgta ttatggaaaa tttttctgta
      121 taatagattc aacaaacaga caatctggtc tgtttgtatt atagctgtca ccggatgtgc
      181 tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt tgtttaatac
      241 tagagaaaga ggggaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg
      301 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      361 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accacaggca
      421 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg
      481 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct
      541 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      601 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      661 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      721 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      781 aggacggcag cgtgcagctc gccgaccact accagcagaa caccccaatc ggcgacggcc
      841 ccgtgctgct gcccgacaac cactacctta gctaccagtc cgccctgagc aaagacccca
      901 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      961 gcatggacga gctgtacaag taactcggta ccaaattcca gaaaagaggc ctcccgaaag
     1021 gggggccttt tttcgttttg gtccaatggc ggcgcgccat cgaatgaggt gcttcctcgc
     1081 tcactgactc gctgcacgag gcagacctca gcgctagcgg agtgtatact ggcttactat
     1141 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     1201 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     1261 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     1321 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     1381 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     1441 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     1501 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     1561 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     1621 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1681 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     1741 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     1801 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     1861 ccccgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     1921 caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaaatc aatctaaagt
     1981 atatatgagt aaacttggtc tgacagttac cttagaaaaa ctcatcgagc atcaaatgaa
     2041 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     2101 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     2161 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2221 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     2281 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     2341 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     2401 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     2461 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     2521 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     2581 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     2641 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     2701 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     2761 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     2821 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     2881 atatattttt atcttgtgca atgtacatca gagattttga gacacaa
//
