LOCUS       pAJM.713                2892 bp ds-DNA     circular     13-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513113074850.353
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     misc_feature    73..92
                     /note="Geneious type: operator"
                     /label="LuxO"
     promoter        73..127
                     /label="pLuxB"
     promoter        93..97
                     /label="-35"
     promoter        114..119
                     /label="-10"
     misc_feature    128..202
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    211..222
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    229..948
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    949..1009
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     rep_origin      1069..1905
                     /label="more\stringent\p15A"
     rep_origin      1342
                     /label="origin"
     CDS             complement(1977..2792)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttacagtc
      121 gaataaaagc tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc
      181 ctctacaaat aattttgttt aatactagag aaagagggga aatactagat ggtgagcaag
      241 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      301 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      361 ctgaagttca tctgcaccac aggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      421 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
      481 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      541 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      601 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      661 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      721 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      781 cagaacaccc caatcggcga cggccccgtg ctgctgcccg acaaccacta ccttagctac
      841 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      901 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact cggtaccaaa
      961 ttccagaaaa gaggcctccc gaaagggggg ccttttttcg ttttggtcca atggcggcgc
     1021 gccatcgaat gaggtgcttc ctcgctcact gactcgctgc acgaggcaga cctcagcgct
     1081 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     1141 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     1201 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     1261 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     1321 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     1381 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1441 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     1501 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     1561 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     1621 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     1681 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     1741 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     1801 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     1861 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca
     1921 gtggaacgaa aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccttag
     1981 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     2041 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     2101 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2161 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2221 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     2281 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2341 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     2401 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     2461 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     2521 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     2581 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2641 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     2701 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     2761 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     2821 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta catcagagat
     2881 tttgagacac aa
//
