LOCUS       pAJM.714                2887 bp ds-DNA     circular     13-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513113085033.367
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     misc_feature    73..84
                     /note="Geneious type: operator"
                     /label="VanO"
     promoter        73..122
                     /label="pVanCC"
     promoter        84..89
                     /label="-35"
     promoter        107..112
                     /label="-10"
     misc_feature    111..122
                     /note="Geneious type: operator"
                     /label="VanO"
     promoter        119
                     /label="+1"
     misc_feature    123..197
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    206..217
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    224..943
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    944..1004
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     rep_origin      1064..1900
                     /label="more\stringent\p15A"
     rep_origin      1337
                     /label="origin"
     CDS             complement(1972..2787)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgattggatc caattgacag ctagctcagt cctaggtacc attggatcca
      121 atagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta
      181 caaataattt tgtttaatac tagagaaaga ggggaaatac tagatggtga gcaagggcga
      241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      361 gttcatctgc accacaggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      421 ctacggcctg caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa
      481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      781 caccccaatc ggcgacggcc ccgtgctgct gcccgacaac cactacctta gctaccagtc
      841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      901 cgccgccggg atcactctcg gcatggacga gctgtacaag taactcggta ccaaattcca
      961 gaaaagaggc ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat
     1021 cgaatgaggt gcttcctcgc tcactgactc gctgcacgag gcagacctca gcgctagcgg
     1081 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
     1141 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
     1201 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     1261 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     1321 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1381 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1441 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     1501 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     1561 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     1621 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     1681 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     1741 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     1801 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     1861 cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac gctcagtgga
     1921 acgaaaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac cttagaaaaa
     1981 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     2041 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     2101 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     2161 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     2221 tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     2281 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     2341 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     2401 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     2461 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     2521 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     2581 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     2641 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
     2701 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca
     2761 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
     2821 cagttttatt gttcatgatg atatattttt atcttgtgca atgtacatca gagattttga
     2881 gacacaa
//
