LOCUS       pAJM.717                2913 bp ds-DNA     circular     13-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513115595081.488
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     promoter        73..148
                     /label="pTet*"
     misc_feature    95..113
                     /note="Geneious type: operator"
                     /label="TetO2"
     promoter        114..119
                     /label="-35"
     misc_feature    120..138
                     /note="Geneious type: operator"
                     /label="TetO2"
     promoter        137..142
                     /label="-10"
     misc_feature    149..223
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    232..243
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    250..969
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    970..1030
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     rep_origin      1090..1926
                     /label="more\stringent\p15A"
     rep_origin      1363
                     /label="origin"
     CDS             complement(1998..2813)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgttttcagc aggacgcact gacctcccta tcagtgatag agattgacat
      121 ccctatcagt gatagagata ctgagcacag ctgtcaccgg atgtgctttc cggtctgatg
      181 agtccgtgag gacgaaacag cctctacaaa taattttgtt taatactaga gaaagagggg
      241 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      301 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      361 gccacctacg gcaagctgac cctgaagttc atctgcacca caggcaagct gcccgtgccc
      421 tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac
      481 cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      541 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      601 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      661 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      721 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      781 cagctcgccg accactacca gcagaacacc ccaatcggcg acggccccgt gctgctgccc
      841 gacaaccact accttagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      901 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      961 tacaagtaac tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc
     1021 gttttggtcc aatggcggcg cgccatcgaa tgaggtgctt cctcgctcac tgactcgctg
     1081 cacgaggcag acctcagcgc tagcggagtg tatactggct tactatgttg gcactgatga
     1141 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     1201 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     1261 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     1321 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     1381 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     1441 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     1501 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     1561 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     1621 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     1681 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     1741 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     1801 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     1861 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     1921 tattaagggg tctgacgctc agtggaacga aaaatcaatc taaagtatat atgagtaaac
     1981 ttggtctgac agttacctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     2041 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     2101 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     2161 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     2221 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     2281 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     2341 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     2401 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     2461 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     2521 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     2581 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     2641 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     2701 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     2761 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc
     2821 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     2881 tgtgcaatgt acatcagaga ttttgagaca caa
//
