LOCUS       pAJM.718                2900 bp ds-DNA     circular     13-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513116376094.538
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     promoter        73..135
                     /label="pBetI"
     promoter        101..106
                     /label="-35"
     misc_feature    107..128
                     /note="Geneious type: operator"
                     /label="BetO"
     promoter        124..129
                     /label="-10"
     misc_feature    136..210
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    219..230
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    237..956
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    957..1017
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     rep_origin      1077..1913
                     /label="more\stringent\p15A"
     rep_origin      1350
                     /label="origin"
     CDS             complement(1985..2800)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgagcgcggg tgagagggat tcgttaccaa tagacaattg attggacgtt
      121 caatataatg ctagcagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac
      181 gaaacagcct ctacaaataa ttttgtttaa tactagagaa agaggggaaa tactagatgg
      241 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      301 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      361 agctgaccct gaagttcatc tgcaccacag gcaagctgcc cgtgccctgg cccaccctcg
      421 tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc
      481 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      541 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      601 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      661 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      721 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      781 actaccagca gaacacccca atcggcgacg gccccgtgct gctgcccgac aaccactacc
      841 ttagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      901 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaactcg
      961 gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt ttggtccaat
     1021 ggcggcgcgc catcgaatga ggtgcttcct cgctcactga ctcgctgcac gaggcagacc
     1081 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     1141 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     1201 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     1261 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     1321 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     1381 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     1441 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     1501 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     1561 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
     1621 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
     1681 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
     1741 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
     1801 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
     1861 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taaggggtct
     1921 gacgctcagt ggaacgaaaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1981 taccttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     2041 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     2101 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     2161 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     2221 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     2281 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     2341 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     2401 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     2461 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     2521 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     2581 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     2641 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     2701 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     2761 gcggcctcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     2821 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaca
     2881 tcagagattt tgagacacaa
//
