LOCUS       pAJM.1459               2930 bp ds-DNA     circular     13-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513181359281.457
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     misc_feature    73..118
                     /note="Geneious type: operator"
                     /label="PcaO"
     promoter        73..165
                     /label="p3B5C"
     promoter        129..134
                     /label="-35"
     promoter        153..158
                     /label="-10"
     misc_feature    166..240
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    249..260
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    267..986
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    987..1047
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     rep_origin      1107..1943
                     /label="more\stringent\p15A"
     rep_origin      1380
                     /label="origin"
     CDS             complement(2015..2830)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgttttgttc gattatcgaa caaattattg aaatatcgaa caaaacctct
      121 aaactactgt ggcactgaat caaaaaatta taaaccctga tcagaagctg tcaccggatg
      181 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa
      241 tactagagaa agaggggaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg
      301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccacag
      421 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct
      481 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag
      541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacacccca atcggcgacg
      841 gccccgtgct gctgcccgac aaccactacc ttagctacca gtccgccctg agcaaagacc
      901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      961 tcggcatgga cgagctgtac aagtaactcg gtaccaaatt ccagaaaaga ggcctcccga
     1021 aaggggggcc ttttttcgtt ttggtccaat ggcggcgcgc catcgaatga ggtgcttcct
     1081 cgctcactga ctcgctgcac gaggcagacc tcagcgctag cggagtgtat actggcttac
     1141 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
     1201 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
     1261 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     1321 tcatggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     1381 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     1441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
     1501 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
     1561 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     1621 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1681 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
     1741 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
     1801 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
     1861 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
     1921 tctcaagaag atcatcttat taaggggtct gacgctcagt ggaacgaaaa atcaatctaa
     1981 agtatatatg agtaaacttg gtctgacagt taccttagaa aaactcatcg agcatcaaat
     2041 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     2101 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     2161 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     2221 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     2281 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     2341 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     2401 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     2461 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     2521 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     2581 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     2641 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     2701 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     2761 ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa
     2821 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     2881 atgatatatt tttatcttgt gcaatgtaca tcagagattt tgagacacaa
//
