LOCUS       pAJM.773                3951 bp ds-DNA     circular     12-DEC-2017
DEFINITION  .
ACCESSION   urn.local...1513120129029.668
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /note="Geneious type: terminator"
                     /label="L3S3P21"
     misc_feature    73..84
                     /note="Geneious type: operator"
                     /label="VanO"
     promoter        73..122
                     /label="pVanCC"
     promoter        84..89
                     /label="-35"
     promoter        107..112
                     /label="-10"
     misc_feature    111..122
                     /note="Geneious type: operator"
                     /label="VanO"
     promoter        119
                     /label="+1"
     misc_feature    123..197
                     /note="Geneious type: insulator"
                     /label="RiboJ"
     misc_feature    206..217
                     /note="Geneious type: RBS"
                     /label="B0064"
     misc_feature    224..943
                     /note="Geneious type: CDS"
                     /label="YFP"
     misc_feature    944..1004
                     /note="Geneious type: terminator"
                     /label="L3S2P21"
     misc_feature    1009..1062
                     /note="Geneious type: promoter"
                     /label="pLacIQ"
     promoter        1027..1032
                     /label="-35"
     promoter        1050..1055
                     /label="-10"
     RBS             1063..1091
                     /label="van2"
     CDS             1092..1826
                     /label="VanR-AM"
     misc_feature    1236..1238
                     /note="Geneious type: mutation"
                     /label="T49I"
     misc_feature    1422..1424
                     /note="Geneious type: mutation"
                     /label="A111V"
     misc_feature    1626..1628
                     /note="Geneious type: mutation"
                     /label="P179S"
     misc_feature    1836..2090
                     /note="Geneious type: terminator"
                     /label="induction operon terminator region"
     rep_origin      2128..2964
                     /label="more\stringent\p15A"
     rep_origin      2401
                     /label="origin"
     CDS             complement(3036..3851)
                     /label="Kanamycin\phosphotransferase"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgattggatc caattgacag ctagctcagt cctaggtacc attggatcca
      121 atagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta
      181 caaataattt tgtttaatac tagagaaaga ggggaaatac tagatggtga gcaagggcga
      241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      361 gttcatctgc accacaggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      421 ctacggcctg caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa
      481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      781 caccccaatc ggcgacggcc ccgtgctgct gcccgacaac cactacctta gctaccagtc
      841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctactgg agttcgtgac
      901 cgccgccggg atcactctcg gcatggacga gctgtacaag taactcggta ccaaattcca
      961 gaaaagaggc ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat
     1021 cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
     1081 gggggtgaat aatggacatg cctcgtatta aaccgggtca gcgtgttatg atggcactgc
     1141 gtaaaatgat tgcaagcggt gaaatcaaaa gtggtgaacg tattgcagaa attccgaccg
     1201 cagcagcact gggtgttagc cgtatgccgg ttcgtatcgc actgcgttca ctggaacaag
     1261 aaggtctggt tgttcgtctg ggtgcacgtg gttatgcagc ccgtggtgtt agcagcgatc
     1321 agattcgtga tgcaattgaa gttcgtggtg ttctggaagg ttttgcagca cgtcgtctgg
     1381 cagaacgtgg tatgaccgca gaaacccatg cacgttttgt tgtactgatt gcagaaggtg
     1441 aagcactgtt tgcagccggt cgcctgaatg gtgaagatct ggatcgttat gccgcatata
     1501 atcaggcatt tcatgatacc ctggttagcg cagcaggtaa tggtgcagtt gaaagcgcac
     1561 tggcacgtaa tggttttgaa ccgtttgcag cagccggtgc actggccctg gatctgatgg
     1621 acctgtctgc cgaatatgaa catctgctgg cagcacatcg tcagcatcag gcagttctgg
     1681 atgcagttag ctgtggtgat gccgaaggtg cagaacgtat tatgcgtgat catgcactgg
     1741 cagcaattcg taatgcaaaa gtttttgaag cagcagcaag cgcaggcgca ccgctgggtg
     1801 cagcatggtc aattcgtgca gattgataag gatcctaatt ggtaacgaat cagacaattg
     1861 acggctcgag ggagtagcat agggtttgca gaatccctgc ttcgtccatt tgacaggcac
     1921 attatgcatc gatgataagc tgtcaaacat gagcagatcc tctacgccag acgcatcgtg
     1981 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     2041 ggggaagatc gggctcgcca cttcgggctc atgagcaaat attttatctg aggtgcttcc
     2101 tcgctcactg actcgctgca cgaggcagac ctcagcgcta gcggagtgta tactggctta
     2161 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
     2221 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
     2281 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
     2341 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
     2401 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
     2461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
     2521 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
     2581 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
     2641 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2701 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
     2761 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
     2821 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
     2881 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
     2941 atctcaagaa gatcatctta ttaaggggtc tgacgctcag tggaacgaaa aatcaatcta
     3001 aagtatatat gagtaaactt ggtctgacag ttaccttaga aaaactcatc gagcatcaaa
     3061 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     3121 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     3181 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     3241 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     3301 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     3361 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     3421 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     3481 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     3541 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     3601 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     3661 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     3721 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3781 tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga
     3841 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     3901 gatgatatat ttttatcttg tgcaatgtac atcagagatt ttgagacaca a
//
